Bax-induction alone is sufficient to activate apoptosis cascade in wild-type Bax-bearing K562 cells, and the initiation of apoptosis requires simultaneous caspase activation

Kobayashi, Toshimitsu; Sawa, Hidehiko; Morikawa, Jun; Ueno, S.; Katayama, Naoyuki; Zhang, Wei; Shiku, Hiroshi

doi:10.3892/ijo.20.4.723

Cited by 6 publications

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“…The present study did not find any change in Bcl-2, an antiapoptotic protein, expression but levels of Bax, which is a proapoptotic protein, were lower in the PD-ECGF/TP-treated group than in pCI or Saline groups. It has been shown that the Bax protein, when present above a threshold level, triggers the apoptosis cascade (16). Our data also suggest that direct myocardial injection of the PD-ECGF/TP gene inhibited myocardial apoptosis, but the mechanism is not clear, whether it is via inhibiting Bax expression should be discussed further.…”

Section: Discussionmentioning

confidence: 84%

Gene therapy for chronic myocardial ischemia using platelet-derived endothelial cell growth factor in dogs

Tanaka

Ihaya

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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Section: Discussionmentioning

confidence: 84%

Gene therapy for chronic myocardial ischemia using platelet-derived endothelial cell growth factor in dogs

Tanaka

Ihaya

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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“…This system is most suited, since previous studies demonstrated that tet-regulated overexpression of apoptotic genes mediates cell death in transfected cancer cells. Thus, tet-induced overexpression of BAX enhanced apoptosis induced by chemotherapeutic agents (Kobayashi et al, 2002). Similarly, Faris et al (1998) utilised the Tet-Off system for overexpression of a dominant-active MEKK1 molecule, which mediated apoptosis by upregulation of CD95-L.…”

Section: Discussionmentioning

confidence: 99%

Induction of apoptosis in experimental human B cell lymphomas by conditional TRAIL-expressing T cells

et al. 2003

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In the present study, we demonstrate the utility of a non-tumour-forming T-cell line for the inducible gene transfer of tumour necrosis factor (TNF)-related apoptosis-inducing ligand (Apo2L/TRAIL), which has been shown to selectively induce apoptosis in malignant but not in normal cells. To generate T cells inducible for TRAIL expression, we stably transfected Jurkat cells with TRAIL in the context of the Tet-On system. The switched on cells strongly expressed TRAIL mRNA, whose protein product was expressed on the cell surface. Paracrine induction of apoptosis in human target tumour cells was solely found for membrane-bound TRAIL. The Jurkat-TRAIL cells itself survived due to clonal selection of TRAIL-resistant cells. Jurkat-TRAIL cells had an additive effect with cytotoxic drugs in vitro, since cell death was enhanced. To elucidate the antitumoral activity of these Jurkat-TRAIL cells in vivo, we injected them intratumorally in xenografts of human Burkitt lymphomas. Switching on expression of TRAIL by adding tetracycline to the drinking water of the mice strongly reduced tumour growth by apoptosis in a caspase-dependent manner. Thus, non-tumourforming T-cell lines offer a novel method for gene transfer and inducible expression of TRAIL in tumour therapy.

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“…Previously, we had shown that matrine treatment did not alter the expression of Bcl-xL, but up-regulated Bax protein level and caused the release of cytochrome C in K562 cells [25]. It has been reported that Bax-induction alone is sufficient to activate the apoptosis cascade in some cell types [26]. Our results imply that matrine may induce Bcl-2 and suppress Bax expression and thereby inhibit the growth of H22 hepatocarcinoma cells in vitro and in vivo but more work is required to further clarify the mechanisms of matrine-induced apoptosis.…”

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confidence: 90%

Anticancer Effects of the Chinese Medicine Matrine on Murine Hepatocellular Carcinoma Cells

et al. 2008

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Matrine is a component of the traditional Chinese medical herb Sophora flavescens Ait, which is widely used to treat diseases such as viral hepatitis, cardiac arrhythmia and skin inflammations. As indicated by previous reports, the molecular mechanism of matrine's anti-cancer effect has been poorly clarified. In this study, we used both in vitro and in vivo models to investigate matrine's antitumor effect and its possible molecular mechanisms. Murine hepatocellular carcinoma H22 cells were cultured in the presence of matrine at various concentrations (0.2 - 2.0 mg/mL). A dose-dependent antiproliferation effect was observed. The 50 % inhibitory concentration (IC (50)) was 0.6 mg/mL. Antiproliferation effects of matrine were associated with an increase in cells arrested in the G (1) phase of the cell cycle. Morphological changes, flow cytometric analysis and expression of the proapoptotic protein Bax indicated that this anticancer effect was mediated via apoptosis. In vivo antitumor efficacy was evaluated following S. C. inoculation of H22 cells in BALB/c mice. Matrine administrated I. P. resulted in strong in vivo anticancer activity. Our results showed that seven doses of matrine at 50 mg/kg/dose inhibited 60.7 % of tumor growth. Transmission electron microscope (TEM) analysis and histoimmunochemical staining for Bcl-2 and Bax proteins also indicated induction of apoptosis in tumor tissues by matrine. Taken together, our results demonstrate that matrine possesses strong antitumor activities in vitro and in vivo. Inhibition of cell proliferation and induction of apoptosis are the likely mechanisms responsible for matrine's antitumor activities.

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Bax-induction alone is sufficient to activate apoptosis cascade in wild-type Bax-bearing K562 cells, and the initiation of apoptosis requires simultaneous caspase activation

Cited by 6 publications

References 0 publications

Gene therapy for chronic myocardial ischemia using platelet-derived endothelial cell growth factor in dogs

Gene therapy for chronic myocardial ischemia using platelet-derived endothelial cell growth factor in dogs

Induction of apoptosis in experimental human B cell lymphomas by conditional TRAIL-expressing T cells

Anticancer Effects of the Chinese Medicine Matrine on Murine Hepatocellular Carcinoma Cells

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