Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

Abstract

The present study evaluates the effect of olive oil-derived antioxidants, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), on cryopreserved caprine sperm using Bayesian inference of ANOVA. For this proposal, sperm was collected, pooled and diluted in freezing media supplemented with different concentrations of HT, DHPG and the mixture (MIX) of both antioxidants. Sperm motility, viability, acrosome integrity, mitochondrial status, and lipid peroxidation (LPO) were assessed in fresh and frozen-thawed sperm samples. The results provided evidence that HT at low concentrations improves sperm motility and viability, and reduces the LPO. Contrastingly, DHPG and MIX exert a positive effect by reducing LPO values as concentration increases. Additionally, mitochondrial potential was reduced when samples were supplemented with HT at low concentrations and mixture of both antioxidants. Conclusively, the addition of olive oil-derived antioxidants (HT at 10 µg/mL and DHPG at 30 µg/mL) implements a protective effect in cryopreserved buck sperm. Bayesian analysis alternatives offer new possibilities to determine the repercussion of antioxidants on sperm, both quantitatively and qualitatively.

Impact of the Antioxidant Hydroxytyrosol on the Quality of Post-Thawed Stallion Semen

“…There was no significant difference ( p > 0.05) in the characteristics of velocity and relative velocity (VCL, VSL, VAP, LIN, and STR) among groups. This phenomenon, which has a resemblance to a previously observed pattern, has been documented in goats [ 14 ] and rams [ 22 ]. Nevertheless, the use of 5 μ g/ml of HT has the potential to enhance the value of VSL.…”

“…However, elevated levels of these antioxidants may have detrimental effects on sperm. There are a lot of data indicating that increased concentrations may not always result in improved quality, but rather may have detrimental effects [ 14 ]. Therefore, it might be suggested that the HT inhibited H 2 O 2 and decreased its effect on DNA at lower concentrations.…”

“…To our knowledge, this work represents the first investigation into the potential of HT as an antioxidant in the cryopreservation process of stallion sperm. Previous research has examined the impact of antioxidants, such as HT, on the quality of sperm in many animal species, including the semen of bulls [ 12 ], rams [ 13 ], and goats [ 14 ]. Hence, the objectives of this research were to assess the impact of HT antioxidant supplementation to the basic extender (HF-20) on cooled and frozen-thawed sperm parameters of Arabian horses.…”

Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool

“…Some of these substances have antioxidant effects, such as hydroxytyrosol (3,4-dyhydroxyphenylethanol, HT), a phenolic compound that reduces oxidation of low-density lipoproteins, protects against H 2 O 2 cytotoxicity and minimizes lactate dehydrogenase activity ( Fernandez-Bolanos et al, 2008 ; Rubio-Senent et al, 2012 ; Bermúdez-Oria et al, 2019 ; Bermúdez-Oria et al, 2020 ). This substance has been tested in roosters ( Díaz Ruiz et al, 2023a ), and its addition in the diluent has resulted in an improvement of the post-thawing semen quality, as well as in other animal species ( Hamden et al, 2010 ; Arando et al, 2019 ; Zhang et al, 2020b ; Arando Arbulu et al, 2021 ). In addition to HT, there is another minor phenolic compound, 3,4-dihydroxyphenylglycol, which has also been tested for its antioxidant effect in roosters ( Díaz Ruíz et al, 2023b ), sheep ( Arando et al, 2019 ), goats ( Arando Arbulu et al, 2021 ) and dogs ( Shakouri et al, 2021 ).…”

“…This substance has been tested in roosters ( Díaz Ruiz et al, 2023a ), and its addition in the diluent has resulted in an improvement of the post-thawing semen quality, as well as in other animal species ( Hamden et al, 2010 ; Arando et al, 2019 ; Zhang et al, 2020b ; Arando Arbulu et al, 2021 ). In addition to HT, there is another minor phenolic compound, 3,4-dihydroxyphenylglycol, which has also been tested for its antioxidant effect in roosters ( Díaz Ruíz et al, 2023b ), sheep ( Arando et al, 2019 ), goats ( Arando Arbulu et al, 2021 ) and dogs ( Shakouri et al, 2021 ).…”