Bcl-2 and p53 expression in node-negative breast carcinoma: A study with long-term follow-up

Barbareschi, Mattia; Caffo, Orazio; Veronese, Silvio; Leek, Russell; Fina, Paolo; Fox, Stephen B.; Bonzanini, Mariella; Girlando, Salvatore; Morelli, Luca; Eccher, Claudio; Pezzella, Francesco; Doglioni, Claudio; Palma, Paolo Dalla; Harris, Adrian L.

doi:10.1016/s0046-8177(96)90307-x

Cited by 98 publications

(90 citation statements)

References 37 publications

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“…Loss of p53 does not inhibit the expression of ER in normal mammary cells, since ER is expressed in p53-null mammary epithelia (data not shown and Medina et al, 2003). Loss of p53 does not have a significant correlation with the ER status of human breast tumors, and 20% of tumors arising in mammary epithelia from p53-knockout mice express ER (Barbareschi et al, 1996;Medina et al, 2002). To determine if germline p53 loss might modulate the expression of ER in Wnt-1 TG mice, we examined both preneoplastic and tumor sections from mice that were Wnt-1 TG/p53À/À.…”

Section: Resultsmentioning

confidence: 88%

Estrogen receptor positivity in mammary tumors of Wnt-1 transgenic mice is influenced by collaborating oncogenic mutations

et al. 2005

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The majority (75%) of human breast cancers express estrogen receptor (ER). Although ER-positive tumors usually respond to antiestrogen therapies, 30% of them do not. It is not known what controls the ER status of breast cancers or their responsiveness to antihormone interventions. In this report, we document that transgenic (TG) expression of Wnt-1 in mice induces ER-positive tumors. Loss of Pten or gain of Ras mutations during the evolution of tumors in Wnt-1 TG mice has no effect on the expression of ER, but overexpression of Neu or loss of p53 leads to ER-negative tumors. Thus, our results provide compelling evidence that expression of ER in breast cancer may be influenced by specific genetic changes that promote cancer progression. These findings constitute a first step to explore the molecular mechanisms leading to ER-positive or ER-negative mammary tumors. In addition, we find that ER-positive tumors arising in Wnt-1 TG mice are refractory to both ovariectomy and the ER antagonist tamoxifen, but lose ER expression with tamoxifen, suggesting that antiestrogen selects for ERnegative tumor cells and that the ER-positive cell fraction is dispensable for growth of these tumors. This is a first report of a mouse model of antiestrogen-resistant ERpositive breast cancers, and could provide a powerful tool to study the molecular mechanisms that control antiestrogen resistance.

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Section: Resultsmentioning

confidence: 88%

Estrogen receptor positivity in mammary tumors of Wnt-1 transgenic mice is influenced by collaborating oncogenic mutations

et al. 2005

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“…Meanwhile, brain SOD and CAT activities were determined colorimetrically using the kit purchased from Biodiagnostic Co., Egypt, according to the methods of Nishikimi et al [14] and Aebi [11], respectively. Brain Bcl-2 level was detected by ELISA technique using the kit purchased from Bender Med Systems Co., Vienna, Europe, according to the method of Barbareschi et al [15]. While, brain BDNF level was detected by ELISA technique using the kit purchased from R and D system Co., UK, Europe, according to the method of Barakat-Walter [16].…”

Section: Biochemical Analysismentioning

confidence: 99%

Does Melatonin Ameliorate Neurological Changes Associated With Alzheimer’s Disease in Ovariectomized Rat Model?

et al. 2012

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This study aimed to elucidate the mechanisms of melatonin to manage neurological damage in Alzheimer's disease (AD) induced in ovariectomized rats. Forty adult female rats were enrolled in our study and were classified as; gonad intact control, ovariectomized control group, ovariectomized rats received melatonin, ovariectomized rats injected with AlCl 3 to induce AD and ADinduced rats treated with melatonin. Hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), B cell lymphoma 2 (Bcl-2), brain derived neurotrophic factor (BDNF), acetylcholinesterase (AchE) and acetylcholine (Ach) were estimated in the brain tissues of the different groups. Treatment of AD-induced rats with melatonin produced marked improvement in the most studied biomarkers which was confirmed by histological investigation of the brain. In Conclusion, melatonin significantly ameliorates the neurodegeneration characteristic of AD in experimental animal model due to its antioxidant, antiapoptotic, neurotrophic and anti-amyloidogenic activities.

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“…Bcl2 expression can be detected in many hormonally regulated cells, such as epithelia cells of normal breast and ER/PR-positive breast carcinomas. [10,30,31] Furthermore, Bcl2 has been shown to be an important downstream mediator of estrogen action survival of cancer cells. [10] However, in our study, Bcl2 was not found to be significant associated with the expression of steroid hormone receptors such as ER, compared to other studies.…”

Section: Discussionmentioning

confidence: 99%

486 From the 1 Department of Pathology, Faculty of Medicine, University Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia, 2 Department of Surgery, Faculty of Medicine, University Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia, 3 Department of Anatomy, Faculty of Medicine, University Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia Received: Jan. 18, 2011; Accept: Mar. 8, 2012 Correspondence to: Dr. Siti Aishah Md Ali, Department of Pathology, Faculty of Medicine, University

Munawer¹,

Zin²,

Ali³

et al. 2012

Biomedical

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Bcl-2 and p53 expression in node-negative breast carcinoma: A study with long-term follow-up

Cited by 98 publications

References 37 publications

Estrogen receptor positivity in mammary tumors of Wnt-1 transgenic mice is influenced by collaborating oncogenic mutations

Estrogen receptor positivity in mammary tumors of Wnt-1 transgenic mice is influenced by collaborating oncogenic mutations

Does Melatonin Ameliorate Neurological Changes Associated With Alzheimer’s Disease in Ovariectomized Rat Model?

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