Bcl-2/Bax protein and mRNA expression in yak (Bos grunniens) placentomes

Fan, Jinjiang; Yu, Sijiu; Cui, Yan; Xu, Gezhi; Wang, Yunlong; He, Honghong

doi:10.1016/j.theriogenology.2017.07.045

Cited by 16 publications

(14 citation statements)

References 36 publications

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“…When the proteolytic process conserves aspartic residues, procaspase-3 is transformed from the inactive form to the active form (caspase-3). The Bcl-2 family proteins can participate in mitochondrial apoptosis, mainly regarding aspects of apoptosis inhibition and promotion, playing key roles in cell apoptosis [39–41].…”

Section: Resultsmentioning

confidence: 99%

Folate-Functionalized Mesoporous Hollow SnO₂ Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy

et al. 2018

BioMed Research International

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In this study, we prepared PTX-loaded mesoporous hollow SnO2 nanofibers conjugated with folic acid (SFNFP) for liver cancer therapy. According to SEM and TEM characterization, SFNF showed a mesoporous hollow structure. The average outer diameter was 200 nm, and the wall thickness was 50 nm. The DSC and XRD study showed that PTX in the channels of nanofibers was present in an amorphous state. The in vitro release experiments demonstrated that SFNF could efficiently improve the dissolution rate of PTX. Both in vitro cell experiments and in vivo antitumor experiments showed that SFNFP could efficiently inhibit the growth of liver cancer cells. Therefore, SFNF is a promising targeting antitumor drug delivery carrier.

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Section: Resultsmentioning

confidence: 99%

Folate-Functionalized Mesoporous Hollow SnO₂ Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy

et al. 2018

BioMed Research International

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“…Subsequently, total RNA was reverse-transcribed into cDNA with 100 μM oligo(dT) Table 1. A real-time fluorescent quantitative PCR system (LightCycler 480, Roche) was used to perform real-time PCR analysis, as previously described (Fan et al, 2017). Briefly, 200 ng of total cDNA was amplified in a 20 μl reaction mixture containing 10 μl SYBR Premix Dimer Eraser (TaKaRa, RR091Q) and 100 nM of forward and reverse primers.…”

Section: Total Rna Extraction and Mrna Expression Analysesmentioning

confidence: 99%

“…Meanwhile, the female reproductive organs of adult yaks experience cyclic variation during different stages of the reproductive cycle. Ovary follicle development, corpus luteum generation, luteolysis, uterine distention and placentation occur during specific periods (Fan et al., 2017; JiangFeng, Jiu, Wen, & Ben, 2011; Wong & Cheng, 2005). Some reproductive physiological processes are known to be accomplished under hypoxic microenvironments, for example the development of growing follicles for the low‐oxygen delivery efficiency of follicular fluid (Basini et al., 2004; Fischer, Kunzel, Kleinstein, & Gips, 1992), the transformation of ruptured follicles into the corpus luteum just after ovulation (for bleeding and immature vasculature) (Nishimura & Okuda, 2010), and the development of a placenta and foetus of an earlier pregnancy (for the deficiency of maternal blood entering the intervillous space) (Burton, Jauniaux, & Watson, 1999; Jaffe, Jauniaux, & Hustin, 1997).…”

Section: Introductionmentioning

confidence: 99%

The expression of hypoxia‐inducible factor‐1 alpha in primary reproductive organs of the female yak (Bos grunniens) at different reproductive stages

Fan

Han

et al. 2020

Reprod Domestic Animals

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The yak (Bos grunniens) is the most important livestock animal in high-altitude regions owing to its prominent adaptability to cold conditions, nutritional deficiencies and hypoxia. The reproductive organs exhibit different histological appearances and physiological processes at different reproductive stages. Hypoxia-inducible factor-1 alpha (HIF-1α) is the regulatory subunit of HIF-1 that crucially regulates the response to hypoxia in mammalian organisms. The goal of our study was to investigate the expression and distribution of HIF-1α in the primary yak reproductive organs at different reproductive stages. Samples of the ovary, oviduct and uterus of 15 adult female yaks were collected and used in the experiment. The expression and localization of HIF-1α proteins and mRNA were investigated using quantitative real-time polymerase chain reaction (qRT-PCR), Western blot (WB) and immunohistochemistry (IHC). The results indicated that the expression of HIF-1α protein in the ovary was higher during the luteal phase than during the follicular phase and gestation period (p < .05). In the oviduct, HIF-1α protein was also more highly expressed during the luteal phase than during the follicular phase and gestation period (p < .01). However, in the uterus, the HIF-1α protein had stronger expression during the gestation period than during the follicular phase (p < .01) and luteal phase (p < .05). The expression of HIF-1α mRNA was similar to that of its protein. Immunohistochemical analysis revealed intense immunostaining of HIF-1α proteins in the follicular granulosa cells, granular luteal cells, villous epithelial cells of the oviduct, endometrial glandular epithelium and luminal epithelium, foetal villous trophoblast, and epithelia of caruncular crypts. This study showed that the expression of HIF-1α in the ovary, oviduct and uterus varies according to the stage of the reproductive cycle. This implies that HIF-1α plays an important role in regulating the stage-specific physiological function of yak reproductive organs under hypoxic environments.

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“…The predicted product length of these primers was 107, 111, and 187 bp, respectively. Real-time fluorescent quantitative PCR system (Light Cycler 480, Roche, Germany) was used to perform Real Time PCR analysis, as previously described [41]. Briefly, 200 ng of total cDNA was amplified in a 20 µL reaction mixture containing 10 µL SYBR Premix Dimer Eraser (Promega, Mannheim, Germany) and 100 nM of forward and reverse primer.…”

Section: Erk1 and Erk2 Gene Expression Analysismentioning

confidence: 99%

“…Similar to other mammals, the female reproductive organs of adult yaks experience cyclic variation during different stages of their reproductive cycles. Ovary follicle development, corpus luteum generation, luteolysis, uterine distention, and placentation take place at specific periods [22,40,41]. Along with the reproductive cycle, numerous cells, such as ovarian granular cells, luteal cells, endometrial epithelium cells, and endometrial stromal cells experience proliferation or apoptosis [42].…”

Section: Introductionmentioning

confidence: 99%

The Expression of ERK1/2 in Female Yak (Bos grunniens) Reproductive Organs

Fan

Han

et al. 2020

Animals

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The main reproductive organs undergo different histological appearances and physiological processes under different reproductive statuses. The variation of these organs depends on a delicate regulation of cell proliferation, differentiation, and apoptosis. Extracellular signal-regulated kinases1/2 (ERK1/2) are members of the mitogen-activated protein kinase (MAPK) super family. They have important roles in regulating various biological processes of different cells, tissues, and organ types. Activated ERK1/2 generally promotes cell survival, but under certain conditions, ERK1/2 also have the function of inducing apoptosis. It is widely believed that ERK1/2 play a significant role in regulating the reproductive processes of mammals. The goal of our research is to investigate the expression and distribution of ERK1/2 in the yak’s main reproductive organs during different stages. In the present study, samples of the ovary, oviduct, and uterus of 15 adult female yak were collected and used in the experiment. The ERK1/2 proteins, localization, and quantitative expression of their mRNA were investigated using immunohistochemistry (IHC), western blot (WB) and relative quantitative real-time polymerase chain reaction (RT-PCR). The results indicated that ERK1/2 proteins and their mRNA were highly expressed in the ovary of the luteal phase and gestation period, in the oviduct of the luteal phase, and in the uterus of the luteal phase and gestation period. Immunohistochemical analysis revealed a strong distribution of ERK1/2 proteins in follicular granulosa cells, granular luteal cells, villous epithelial cells of the oviduct, endometrial glandular epithelium, and luminal epithelium. These results demonstrated that the expression of ERK1 and ERK2 proteins and their mRNA in the yak’s ovary, oviduct, and uterus varies with the stage of the reproductive cycle. The variation character of ERK1 and ERK 2 expression in the yak’s main reproductive organs during different stages implies that they play an important role in regulating the reproductive function under different physiological statuses.

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Bcl-2/Bax protein and mRNA expression in yak (Bos grunniens) placentomes

Cited by 16 publications

References 36 publications

Folate-Functionalized Mesoporous Hollow SnO₂ Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy

Folate-Functionalized Mesoporous Hollow SnO₂ Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy

The expression of hypoxia‐inducible factor‐1 alpha in primary reproductive organs of the female yak (Bos grunniens) at different reproductive stages

The Expression of ERK1/2 in Female Yak (Bos grunniens) Reproductive Organs

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Bcl-2/Bax protein and mRNA expression in yak (Bos grunniens) placentomes

Cited by 16 publications

References 36 publications

Folate-Functionalized Mesoporous Hollow SnO2 Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy

Folate-Functionalized Mesoporous Hollow SnO2 Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy

The expression of hypoxia‐inducible factor‐1 alpha in primary reproductive organs of the female yak (Bos grunniens) at different reproductive stages

The Expression of ERK1/2 in Female Yak (Bos grunniens) Reproductive Organs

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Product

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Folate-Functionalized Mesoporous Hollow SnO₂ Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy

Folate-Functionalized Mesoporous Hollow SnO₂ Nanofibers as a Targeting Drug Carrier to Improve the Antitumor Effect of Paclitaxel for Liver Cancer Therapy