Bcl-2 functions in an antioxidant pathway to prevent apoptosis

Selective ablation of the YxxM motif of IL-7Rα suppresses lymphomagenesis but maintains lymphocyte development

“…Interestingly, CD4 SP T cells and B cells were not protected from dexamethasone-induced apoptosis (data not shown). However, Tg IL-7 CD8 T cells were still susceptible to free radical-induced apoptosis, despite the finding that ectopic Bcl-2 expression can be protective in this setting (Supplementary Figure 2d; Hockenbery et al, 1993).…”

Evaluation of anti–apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide

“…There are sufficient evidences revealing the vital range of biological activities of antioxidants such as direct or indirect scavenging of free radicals, anticancer, antimutagenesis [2] and anti-apoptotic activity [25] . Although the role of apoptosis in pathological situation for cancer suppression has been already evaluated [9][10][11] .…”

“…Cultured RCC-26 cells (2×10 6 ) were seeded and allowed to adhere in a 96-well plate using RPMI-1640 medium. Then cells were treated with various antioxidants (20 and 50 毺 g/mL) followed by 2 h incubation and 3.5 mM H 2 O 2 was added for another 3 h. After the incubation 25 毺 L of 5 mg/mL MTT (dissolved in PBS) was added to each well followed by incubation for 4 h at 37 曟 . Thereafter, formazan crystals were dissolved in 150 毺 L DMSO followed by the quantitative measurement of formazan purple by the absorbance at 540 nm.…”

“…In brief, cells were cultured for 18 h either in presence or absence of various antioxidants (AA, CA, SA, 毩-TA) followed by exposure of 3.5 mM H 2 O 2 for 3 h. Thereafter, cells were pelleted and incubated at room temperature in the lysis buffer (provided with the kit) for 30 min and centrifuged at 200× g for 10 min. 25 mL of supernatant containing mono and oligonucleosomes (released by treated cells) was used for enzyme-linked immunosorbent assay (ELISA). The results were expressed in terms of an enrichment factor, i.e., ratio of absorbance (A 405 nm/A 490 nm) of treated cells/absorbance (A 405 nm/A 490 nm) of control cells.…”

“…It is documented that antioxidants have been used to inhibit apoptosis since apoptosis is initially thought to be oxidative stress mediated phenomenon [25] . We attempted to observe the changes in DCF fluorescence with fluorescence microscopy ( Figure 9).…”