Bcl-2: Is it an easier way to differentiate psoriasis and eczema?

Yalçın, Özben; Demir, Filiz Topaloğlu; Kılıç, Ayşe; Kaya, Hüseyin; Kabukçuoğlu, Fevziye; Altunay, İlknur Kıvanç

doi:10.5222/otd.2016.1064

Cited by 1 publication

(1 citation statement)

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“…According to the equilibrium in the expression of these members, the cell is directed to apoptosis. Bcl-2, which is the first anti-apoptotic member of the Bcl-2 gene family, is one of the molecules that inhibit apoptosis (11). If there is any damage to the mechanism of apoptosis, the cells tend to become cancerous (12).…”

Section: Discussionmentioning

confidence: 99%

Effect of epidermal growth factor (EGF) on osteosarcoma cell proliferation and Bcl-2 gene expression

Avcıkurt¹,

Altun²

2019

eamr

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Objective: The aim of this study was to investigate the effects of epidermal growth factor (EGF) on osteosarcoma cell proliferation and Bcl-2 expression. Methods: In this study, MTT test was first applied to determine the effect of EGF on cell proliferation. Twenty ng/μL EGF was added to the Saos-2 cell line and the effects on cell proliferation were determined after different incubation intervals. At the next experimental stage, 20 ng/mL EGF was added to the osteosarcoma cell line and the expression of antiapoptotic Bcl-2 was evaluated at 1-, 3-, 24-, 48-and 72-hour. Results: A statistically significant increase in cell proliferation was observed at 3-, 24-and 72-hour after administration of 20 ng/μL EGF in the osteosarcoma cell line. EGF was observed to stimulate Bcl-2 expression at a dose of 20 ng/mL at 1-and 24-hour. Conclusion: EGF; at the 3 rd , 24 th and 72 nd hours, a statistically significant increase in cell proliferation was observed. In addition, the expression of antiapoptotic Bcl-2 was evaluated at the 1 st , 3 rd , 24 th , 48 th and 72 th hours on the EGF osteosarcoma cell line. The expression of Bcl-2 was increased at the end of the 1 st and 24 th hours of application of EGF at 20 ng/mL dose.

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Section: Discussionmentioning

confidence: 99%