bcpmr1 encodes a P-type Ca2+/Mn2+-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea

Plaza, Verónica; Lagües, Yanssuy; Carvajal, Mauro; Perez‐Garcia, Luis; Mora-Montes, Héctor M.; Canessa, Paulo; Larrondo, Luis F.; Castillo, Luís

doi:10.1016/j.fgb.2015.01.012

Cited by 26 publications

(20 citation statements)

References 68 publications

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“…This suggests that this collection of B. cinerea genotypes does not contain large-effect qualitative virulence loci; instead, this generalist uses a highly polygenic virulence architecture (Denby et al, 2004;Finkers et al, 2007b;Rowe and Kliebenstein, 2008;Corwin et al, 2016b;Zhang et al, 2016). This agrees with previous B. cinerea mechanistic studies that identify a large collection of standing genetic variation that controls a wide range of specific virulence mechanisms (Choquer et al, 2007;Rowe and Kliebenstein, 2007;Noda et al, 2010;Rowe et al, 2010;Dalmais et al, 2011;Michielse et al, 2011;Shlezinger et al, 2011;Windram et al, 2012;Pearson and Bailey, 2013;Kumari et al, 2014;An et al, 2015;Atwell et al, 2015;Hevia et al, 2015;Plaza et al, 2015;Schumacher et al, 2015;Zhang et al, 2015a;Corwin et al, 2016aCorwin et al, , 2016bLopezCruz et al, 2017;Zhang et al, 2016). Taken together, this supports the perspective that the Arabidopsis-B.…”

Section: Natural Genetic Variation In B Cinerea Influences Plant Trasupporting

confidence: 83%

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

et al. 2017

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To respond to pathogen attack, selection and associated evolution has led to the creation of plant immune system that are a highly effective and inducible defense system. Central to this system are the plant defense hormones jasmonic acid (JA) and salicylic acid (SA) and crosstalk between the two, which may play an important role in defense responses to specific pathogens or even genotypes. Here, we used the Arabidopsis thaliana-Botrytis cinerea pathosystem to test how the host's defense system functions against genetic variation in a pathogen. We measured defense-related phenotypes and transcriptomic responses in Arabidopsis wild-type Col-0 and JA-and SA-signaling mutants, coi1-1 and npr1-1, individually challenged with 96 diverse B. cinerea isolates. Those data showed genetic variation in the pathogen influences on all components within the plant defense system at the transcriptional level. We identified four gene coexpression networks and two vectors of defense variation triggered by genetic variation in B. cinerea. This showed that the JA and SA signaling pathways functioned to constrain/canalize the range of virulence in the pathogen population, but the underlying transcriptomic response was highly plastic. These data showed that plants utilize major defense hormone pathways to buffer disease resistance, but not the metabolic or transcriptional responses to genetic variation within a pathogen.

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Section: Natural Genetic Variation In B Cinerea Influences Plant Trasupporting

confidence: 83%

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

et al. 2017

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“…The aggregation of A. fumigatus germinating conidia, for example, is dependent on cell wall α-1,3-glucans and may be prevented by the addition of α-1,3-glucanase (Fontaine et al, 2010), and similar phenotypic features have been reported for a B. cinerea mutant in the gene Bcpmr1 displaying an altered cell wall (Plaza et al, 2015). …”

Section: Discussionmentioning

confidence: 77%

BcSUN1, a B. cinerea SUN-Family Protein, Is Involved in Virulence

Pérez‐Hernández

González

et al. 2017

Front. Microbiol.

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BcSUN1 is a glycoprotein secreted by Botrytis cinerea, an important plant pathogen that causes severe losses in agriculture worldwide. In this work, the role of BcSUN1 in different aspects of the B. cinerea biology was studied by phenotypic analysis of Bcsun1 knockout strains. We identified BcSUN1 as the only member of the Group-I SUN family of proteins encoded in the B. cinerea genome, which is expressed both in axenic culture and during infection. BcSUN1 is also weakly attached to the cellular surface and is involved in maintaining the structure of the cell wall and/or the extracellular matrix. Disruption of the Bcsun1 gene produces different cell surface alterations affecting the production of reproductive structures and adhesion to plant surface, therefore reducing B. cinerea virulence. BcSUN1 is the first member of the SUN family reported to be involved in the pathogenesis of a filamentous fungus.

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“…Interestingly, two more B. cinerea ATPases, Bc-CCC2 and Bc-PMR1, were proven to be involved in pathogenicity. 27,25 It needs to be clarified whether the Bc-ATPase from Bcin12G_06020 itself is also required for virulence. At least, sRNA Bc-siR37 from this locus is a bona fide virulence factor.…”

Section: Discussionmentioning

confidence: 99%

Botrytis small RNA Bc-siR37 suppresses plant defense genes by cross-kingdom RNAi

et al. 2017

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Pathogens secrete effector proteins to suppress host immune responses. Recently, we showed that an aggressive plant fungal pathogen Botrytis cinerea can also deliver small RNA effectors into host cells to suppress host immunity. B. cinerea sRNAs (Bc-sRNAs) translocate into host plants and hijack the plant RNAi machinery to induce cross-kingdom RNAi of host immune responsive genes. Here, we functionally characterized another Bc-sRNA effector Bc-siR37 that is predicted to target at least 15 Arabidopsis genes, including WRKY transcription factors, receptor-like kinases, and cell wall-modifying enzymes. Upon B. cinerea infection, the expression level of Bc-siR37 was induced, and at least eight predicted Arabidopsis target genes were downregulated. These target genes were also suppressed in the transgenic Arabidopsis plants overexpressing Bc-siR37, which exhibited enhanced disease susceptibility to B. cinerea. Furthermore, the knockout mutants of the Bc-siR37 targets, At-WRKY7, At-PMR6, and At-FEI2, also exhibited enhanced disease susceptibility to B. cinerea, giving further support that these genes indeed play a positive role in plant defense against B. cinerea. Our study demonstrates that analysis of pathogen sRNA effectors can be a useful tool to help identify host immunity genes against the corresponding pathogen.

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bcpmr1 encodes a P-type Ca2+/Mn2+-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea

Cited by 26 publications

References 68 publications

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

Plastic Transcriptomes Stabilize Immunity to Pathogen Diversity: The Jasmonic Acid and Salicylic Acid Networks within the Arabidopsis/Botrytis Pathosystem

BcSUN1, a B. cinerea SUN-Family Protein, Is Involved in Virulence

Botrytis small RNA Bc-siR37 suppresses plant defense genes by cross-kingdom RNAi

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