BD GeneOhm-MRSA assay for detection of methicillin-resistant<i>Staphylococcus aureus</i>directly in nasal and non-nasal Truro specimens from haematologic patients

Ghebremedhin, Beniam; König, B.; König, Wolfgang

doi:10.1556/eujmi.1.2011.4.5

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…Further evidence came from UBA studies, three of which reported a decrease in the incidence of MRSA acquisition when PCR screening was introduced, [79][80][81] and four of which reported a decrease in reducing TAT. 11,79,[81][82][83] There was strong evidence from a total of 45 studies, 5,[7][8][9][10][11]13,14,16,17,19,[22][23][24]27,[29][30][31][32]35,[37][38][39][40][41]43,45,[47][48][49][50][51]53,57,[58][59][60][61]62,64,65,67,…”

Section: 3mentioning

confidence: 99%

Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Coia

Wilson

Bak

et al. 2021

Journal of Hospital Infection

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Section: 3mentioning

confidence: 99%

Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Coia

Wilson

Bak

et al. 2021

Journal of Hospital Infection

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“…The GENSPEED ® MRSA Test Kit succeeded with this. Several other sampling kits are able to extract DNA from clinical specimens and identify MRSA but detection of the mecC gene is not incorporated yet . Another issue is to handle mixed samples containing MRSE and MSSA.…”

Section: Discussionmentioning

confidence: 99%

Ability of the GENSPEED^®MRSA test kit to detect the novelmecAhomologuemecCinStaphylococcus aureus

Petersen

Medina

Larsen

2015

APMIS

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The aim of this study was to evaluate the GENSPEED(®) MRSA Test Kit that in addition to the traditional MRSA gene mecA, also incorporates a probe for detection of the newly described mecA homologue mecC. So far only one commercial system is able to detect this new gene. The specific objective was to evaluate the ability of the kit to detect and separate Stapyhylococcus aureus containing either mecA, mecC or no methicillin-resistance determinant. Ninety-five MRSA isolates from humans were included in the test and additional mecC-positive isolates from animals and methicillin-sensitive S. aureus were tested. The kit demonstrated 100% sensitivity and 100% specificity compared to a standard PCR method. The kit provides the ability to perform rapid and reliable detection of both mecA-MRSA and mecC-MRSA.

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“…Within the German cohort, the MRSA prevalence was relatively low (n=2/100) which reflects earlier dataset of the University Clinic in Magdeburg. All MRSA and MSSA isolates were negative for PVL gene [11][12][13].…”

Section: Discussionmentioning

confidence: 99%

“…Overall, the study data of the two cohorts indicate geographical differences among nasal colonization by staphylococci, including MSSA and MRSA, and other Gram-positive and -negative bacterial species. S. aureus colonization was less common in Eritrean pediatric cohort; in contrast, the occurrence of this species including low number of methicillin resistant isolates was observed more frequently in the German cohort which corresponds to the local epidemiologic prevalence and clonality of MRSA in the study region [11][12][13]. Controlled, prospective clinical trials in the future might be helpful to determine whether nasal carriage of Gramnegative bacteria in such patients lead to the incidence of infection with these bacterial organisms.…”

Section: Proteus Mirabilis 1 -mentioning

confidence: 93%

Comparative study of nasal bacterial carriage in pediatric patients from two different geographical regions

Ghebremedhin

Köenig

2012

European Journal of Microbiology and Immunology

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The aim of this study was to determine the prevalence of bacterial carriage in the anterior nares of two different patient cohorts, aged 5-15 years. By use of a sensitive enrichment broth, Gram-positive and -negative bacteria were cultured from the two cohorts of each 100 patients at the Referral Clinic in Eritrea and at a German University Hospital. In the German cohort, 27% of the patients were positive either for Gram-negative (n=5) or -positive bacteria, including Staphylococcus aureus (n=8; MRSA (n=2)), Staphylococcus epidermidis (n=12), Corynebacterium spp. (n=4), and Streptococcus pyogenes (n=1). In comparison, the Eritrean cohort revealed 33% bacterial carriers in the anterior nares. Among the identified species were S. aureus (n=2), S. epidermidis (n=13), Streptococcus haemolyticus (n=9), and Gram-negative rods including Klebsiella pneumoniae/oxytoca (n=5), Enterobacter agglomerans (n=4), Escherichia coli (n=2), and Pseudomonas aeruginosa (n=1). Noteworthy, none of the Eritrean patients were positive for MRSA. In both cohorts there was no co-occurrence of Gram-positive and -negative bacteria in the anterior nares. However, we observed in two subjects of the Eritrean cohort co-colonization with S. epidermidis and S. haemolyticus. The occurrence of Gram-negative bacteria was less significant by age in the German cohort, whereas in the Eritrean cohort Gram-negative bacteria were more frequently detected in carriers aged 5-9 years. Continued surveillance of S. aureus and Gram-negative bacteria carriage deserves further attention and might help to determine future trends in the characteristics of nasal carriage, subsequent incidence of infections, and the potential effectiveness of targeted population based intervention.

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BD GeneOhm-MRSA assay for detection of methicillin-resistantStaphylococcus aureusdirectly in nasal and non-nasal Truro specimens from haematologic patients

Cited by 6 publications

References 26 publications

Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Ability of the GENSPEED^®MRSA test kit to detect the novelmecAhomologuemecCinStaphylococcus aureus

Comparative study of nasal bacterial carriage in pediatric patients from two different geographical regions

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BD GeneOhm-MRSA assay for detection of methicillin-resistantStaphylococcus aureusdirectly in nasal and non-nasal Truro specimens from haematologic patients

Cited by 6 publications

References 26 publications

Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Ability of the GENSPEED®MRSA test kit to detect the novelmecAhomologuemecCinStaphylococcus aureus

Comparative study of nasal bacterial carriage in pediatric patients from two different geographical regions

Contact Info

Product

Resources

About

Ability of the GENSPEED^®MRSA test kit to detect the novelmecAhomologuemecCinStaphylococcus aureus