BDE-47 causes developmental retardation with down-regulated expression profiles of ecdysteroid signaling pathway-involved nuclear receptor (NR) genes in the copepod Tigriopus japonicus

Hwang, Dae‐Sik; Han, Jeonghoon; Won, Eun‐Ji; Kim, Duck-Hyun; Jeong, Chang‐Bum; Hwang, Un-Ki; Zhou, Bingsheng; Choe, Joonho; Lee, Jae‐Seong

doi:10.1016/j.aquatox.2016.06.004

Cited by 33 publications

(6 citation statements)

References 41 publications

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“…Thus, indirectly, the chitin biosynthesis pathway is also being coordinated by the 20E‐EcR/USP ligand‐receptor complex (Yao et al 2010). During moulting, the chitin biosynthesis proteins and enzymes such as chitinase (CHT), β‐N‐Acetylglucosaminidase (NAG), chitin deacetylase (CDA), chitin synthase and trehalase (Tre) are crucial for the formation of new epidermal cells after crustaceans shed their exoskeleton and start to synthesise a new cuticle layer (Nagasawa 2012; Hwang et al 2016; Harðardóttir et al 2019). It has been shown that EcR regulates five out of six crucial genes in the chitin biosynthesis pathway of lepidopteron insect Spodoptera exigua (Yao et al 2010).…”

Section: Ecdysone Signalling Pathway and Regulationmentioning

confidence: 99%

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Abdullah‐Zawawi

Afiqah‐Aleng

Ikhwanuddin

et al. 2021

Reviews in Aquaculture

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Ecdysone receptor (EcR) is the primary regulator of the ecdysteroid signalling pathway, a critical pathway that directly links to moulting. In addition, EcR also regulates growth, development, reproduction and regeneration in crustaceans. However, there remains a huge gap of knowledge between the detailed structure and functional role(s) of crustacean EcR compared to that of insects. Motif and phylogenetic analyses of publicly available crustacean EcR proteins revealed the evolutionary relationship of EcR in this subphylum and highlighted its conserved characteristics among crustaceans. The role of EcR in the regulation of essential physiological processes in crustaceans including moulting, chitin synthesis, general growth and development, gonadal and maturation, and limb regeneration was discussed based on the available literature. This essential moult-related nuclear receptor could serve as a useful molecular indicator of moulting and gonadal maturation, as a potential target for pesticide production against parasitic crustacean species in the aquaculture industry, as well as valuable bioindicators of environmental stressors.

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Section: Ecdysone Signalling Pathway and Regulationmentioning

confidence: 99%

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Abdullah‐Zawawi

Afiqah‐Aleng

Ikhwanuddin

et al. 2021

Reviews in Aquaculture

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“…Calanus helgolandicus (Lauritano et al, 2011a(Lauritano et al, ., 2013(Lauritano et al, , 2016 Yes (Lee et al, 2017) Yes Calanus sinicus (Lauritano et al, 2015;Lee et al, 2017) Yes Paracyclopina nana (Han et al, 2015a;Lauritano et al, 2015;Lee et al, 2012Lee et al, , 2016Puthumana et al, 2017) No (Jeong et al, 2015;Lauritano et al, 2011aLauritano et al, , 2013Lauritano et al, , 2016) Yes Tigriopus japonicus (Han et al, 2015a(Han et al, , 2015aHwang et al, 2016;Jeong et al, 2014Jeong et al, , 2015Jeong et al, , 2016Kim et al, 2011Kim et al, , 2013aKim et al, , 2013bKim et al, , 2014Kim et al, , 2015aLee et al, 2012Lee et al, , 2016Puthumana et al, 2017;Rhee et al, 2013;Yi et al, 2014) No Ribosomal protein S16 (S16) Calanus finmarchicus (Aruda et al, 2011;Roncalli et al, 2016b (Lauritano et al, 2011a(Lauritano et al, ., 2013(Lauritano et al, , 2015(Lauritano et al, , 2016) Yes Calanus sinicus (Lauritano et al, 2015) Yes…”

Section: Accepted M M a N U mentioning

confidence: 99%

Molecular physiology of copepods - from biomarkers to transcriptomes and back again

Tarrant

Nilsson

Hansen

2019

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Planktonic copepods are a diverse and abundant group of small (⁓mm sized) aquatic animals that play a critical role in linking the base of the food chain with higher trophic levels. These invertebrates are a primary food source for marine fish larvae. Their ubiquitous presence is thus of vital importance for recruitment of fish stocks and also as promising live feed for finfish production in aquaculture. This paper reviews the application of molecular approaches to understanding copepod physiology, particularly in non-parasitic species. The review includes both targeted gene approaches and untargeted transcriptomic approaches, with suggestions for best practices in each case. Issues particularly relevant to studies of copepods include heterogeneity within species, morphologically cryptic species, experimental artifacts associated with sample handling, and limited annotation of copepod genes and transcripts. The emergence of high-throughput sequencing and associated increased availability of genomic and transcriptomic databases has presented a huge opportunity to advance knowledge of copepod physiology. The research community can leverage this opportunity through efforts to maintain or improve data accessibility, database annotation, and documentation of analytical pipelines.

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“…For this, three representative chemicals, benzo[a]pyrene (B[a]P), bisphenol A (BPA), and mercury (Hg), were selected. In particular, we focused on their growth ages, as this experimental species has a molting process with different sensitivities to growth 31 , 32 . Therefore, we investigated the stability of the nine candidate reference genes under different ages (24-h, 4-day, 7-day, and 10-day) and measured the relative expression of target genes based on the conditions of reference genes.…”

Section: Introductionmentioning

confidence: 99%

Validation of reference genes for quantitative real-time PCR in chemical exposed and at different age’s brackish water flea Diaphanosoma celebensis

Lee

Cho

Kim

et al. 2021

Sci Rep

Self Cite

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Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), a primary approach for evaluating gene expression, requires an appropriate normalization strategy to confirm relative gene expression levels by comparison, and rule out variations that might occur in analytical procedures. The best option is to use a reference gene whose expression level is stable across various experimental conditions to compare the mRNA levels of a target gene. However, there is limited information on how the reference gene is differentially expressed at different ages (growth) in small invertebrates with notable changes such as molting. In this study, expression profiles of nine candidate reference genes from the brackish water flea, Diaphanosoma celebensis, were evaluated under diverse exposure to toxicants and according to growth. As a result, four different algorithms showed similar stabilities of genes for chemical exposures in the case of limited conditions using the same developmental stage (H2A was stable, whereas Act was fairly unstable in adults), while the results according to age showed a significantly different pattern in suite of candidate reference genes. This affected the results of genes EcRA and GST, which are involved in development and detoxification mechanisms, respectively. Our finding is the first step towards establishing a standardized real-time qRT-PCR analysis of this environmentally important invertebrate that has potential for aquatic ecotoxicology, particularly in estuarine environments.

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BDE-47 causes developmental retardation with down-regulated expression profiles of ecdysteroid signaling pathway-involved nuclear receptor (NR) genes in the copepod Tigriopus japonicus

Cited by 33 publications

References 41 publications

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Molecular physiology of copepods - from biomarkers to transcriptomes and back again

Validation of reference genes for quantitative real-time PCR in chemical exposed and at different age’s brackish water flea Diaphanosoma celebensis

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