Bdellovibrio bacteriovorus directly attacks Pseudomonas aeruginosa and Staphylococcus aureus Cystic fibrosis isolates

Iebba, Valerio; Totino, Valentina; Santangelo, Floriana; Gagliardi, Antonella; Ciotoli, L.; Virga, A.; Ambrosi, Cecilia; Pompili, Monica; Biase, Riccardo Valerio De; Selan, Laura; Artini, Marco; Pantanella, Fabrizio; Mura, Francesco; Passariello, Claudio; Nicoletti, Mauro; Nencioni, Lucia; Trancassini, Maria; Quattrucci, Serena; Schippa, Serena

doi:10.3389/fmicb.2014.00280

Cited by 85 publications

(76 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S1). Previously, there had been no evidence that Grampositive bacteria were susceptible to BALOs; recently, however, Bdellovibrio bacteriovorus HD 100 has been demonstrated to decrease numbers of Staphylococcus aureus in broth culture (Iebba et al 2014). In the present study, only 2 out of 9 Bacteriovorax spp.…”

Section: Discussioncontrasting

confidence: 58%

“…In shrimp farming, the molting process of shrimp causes an accumulation of bacterial biofilms at the bottom of the pond. Iebba et al (2014) re ported that BALOs could target their prey both in water and in biofilm. In the current work, we demonstrated the potential of BV-A to significantly diminish biofilm formed by AHPND Vp and by clinical and environmental V. parahaemolyticus isolates (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In the epibiotic life cycle, predators attach to the prey surface and perform binary fission on the outer surface of the prey cell while prey cytoplasmic contents de crease. Iebba et al (2014) demonstrated that B. bacteriovorus HD 100 attacked S. aureus in the epibiotic style. In addition, Monnappa et al (2014) reported extracellular protease released from B. bacteriovorus HD 100 to degrade S. aureus biofilm and reduce its virulence.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Kongrueng¹,

Mitraparp-Arthorn²,

Bangpanwimon³

et al. 2017

Dis. Aquat. Org.

View full text Add to dashboard Cite

Acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by Vibrio parahaemolyticus (AHPND Vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to Photorhabdus insect-related toxins. We obtained 9 isolates of Bdellovibrio and like organisms (BALOs) from water and sediment samples in Thailand. Using 16S rRNA sequencing, all of the organisms were identified as Bacteriovorax spp. and were able to attack all tested AHPND Vp isolates. In addition, their various susceptible hosts, including Gram-positive and Gram-negative bacteria, were observed. The optimal ratio for interaction between the Bacteriovorax isolate BV-A and AHPND Vp was determined to be 1:10. The suitable conditions applied for co-culture between BV-A and AHPND Vp were 30°C, 2% NaCl, and pH 7.6. The capability of BV-A to reduce numbers of AHPND Vp in vitro was observed in co-culture after incubation for 2 d and continued until the end of the incubation period. In vivo, BV-A was able to reduce mortality of shrimp post-larvae infected with AHPND Vp. In addition, BV-A significantly decreased the formation of biofilm by AHPND Vp. These findings provide evidence for using Bacteriovorax as a biocontrol of AHPND Vp in shrimp aquaculture.

show abstract

Section: Discussioncontrasting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Kongrueng¹,

Mitraparp-Arthorn²,

Bangpanwimon³

et al. 2017

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

“…Other uses involve the treatment of various infections in animals, including bovine keratoconjunctivitis caused by Moraxella bovis (5), Aeromonas hydrophila in fish (6,7), Vibrio cholerae and Proteus penneri in shrimp (8,9), and Salmonella enterica serovar Enteritidis in the chicken gut (10). They have also been applied in the treatment of human disease pathogens, including Pseudomonas aeruginosa from the lungs of cystic fibrosis patients (11), Pseudomonas aeruginosa and Serratia marcescens associated with eye infections (12), and oral pathogens that cause periodontal diseases (13,14). Bdellovibrio strains were also effective in the treatment of drug-resistant bacteria (15,16) and in reducing the levels of Escherichia coli and Salmonella spp.…”

mentioning

confidence: 99%

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Richards

Fay

Uknalis

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

c Halobacteriovorax (formerly Bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. Four strains of Halobacteriovorax originally isolated in Vibrio parahaemolyticus O3:K6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. This technique was essential, since 0.45-m filtration alone was unable to remove infectious Vibrio minicells, as determined by scanning electron microscopy and cultural methods. Purified Halobacteriovorax strains were screened for predation against other V. parahaemolyticus strains and against Vibrio vulnificus, Vibrio alginolyticus, Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium DT104, all potential threats to seafood safety. They showed high host specificity and were predatory only against strains of V. parahaemolyticus. In addition, strains of Halobacteriovorax that were predatory for E. coli O157:H7 and S. Typhimurium DT104 were isolated from a tidal river at 5 ppt salinity. In a modified plaque assay agar, they killed their respective prey over a broad range of salinities (5 to 30 ppt). Plaques became smaller as the salinity levels rose, suggesting that the lower salinities were optimal for the predators' replication. These species also showed broader host specificity, infectious against each other's original hosts as well as against V. parahaemolyticus strains. In summary, this study characterized strains of Halobacteriovorax which may be considered for use in the development of broad-based biocontrol technologies to enhance the safety of commercially marketed shellfish and other foods. Bdellovibrio and like organisms (BALO) are Gram-negative, aerobic bacteria which are predatory toward other Gram-negative bacteria and consist of freshwater/terrestrial and marine forms. Bdellovibrio and like organisms are divided into four genera, Bdellovibrio, Bacteriolyticum, Peredibacter, and Bacteriovorax, which was recently renamed Halobacteriovorax (1). These organisms enter into a susceptible host bacterium and reside within the periplasmic space where they utilize the cytoplasmic nutrients of the host to support growth and replication. The replicative form, known as a bdelloplast, elongates within the host and septates into progeny cells as the host is lysed, releasing cells capable of attacking more prey. Halobacteriovorax organisms are the marine forms, which are small, polar flagellated, highly motile, intracellular predators in the class Deltaproteobacteria family Halobacteriovoraceae (1).Bdellovibrio and like organisms, particularly the nonhalophilic Bdellovibrio bacteriovorus, have been proposed for use in reducing the levels of phytopathogens associated with bacterial blights in soybeans and rice (2, 3) and reducing the levels of Salmonella enterica serovar Typhimurium on freshwater fish fillets (4). Other uses involve the treatment of various infections in animals, including bovine keratoconjunctivitis caused by Mora...

show abstract

“…Bacteriovorax spp. are found in animal gut and marine environments; until recently, they were thought to prey exclusively on Gram-negative bacteria, but new evidence suggests that they may also be capable of preying on Gram-positive bacteria (85). Saprospira spp.…”

Section: Discussionmentioning

confidence: 99%

Effects of Actinomycete Secondary Metabolites on Sediment Microbial Communities

Patin

Schorn

Aguinaldo

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

Marine sediments harbor complex microbial communities that remain poorly studied relative to other biomes such as seawater. Moreover, bacteria in these communities produce antibiotics and other bioactive secondary metabolites, yet little is known about how these compounds affect microbial community structure. In this study, we used next-generation amplicon sequencing to assess native microbial community composition in shallow tropical marine sediments. The results revealed complex communities comprised of largely uncultured taxa, with considerable spatial heterogeneity and known antibiotic producers comprising only a small fraction of the total diversity. Organic extracts from cultured strains of the sedimentdwelling actinomycete genus Salinispora were then used in mesocosm studies to address how secondary metabolites shape sediment community composition. We identified predatory bacteria and other taxa that were consistently reduced in the extract-treated mesocosms, suggesting that they may be the targets of allelopathic interactions. We tested related taxa for extract sensitivity and found general agreement with the culture-independent results. Conversely, several taxa were enriched in the extract-treated mesocosms, suggesting that some bacteria benefited from the interactions. The results provide evidence that bacterial secondary metabolites can have complex and significant effects on sediment microbial communities.IMPORTANCE Ocean sediments represent one of Earth's largest and most poorly studied biomes. These habitats are characterized by complex microbial communities where competition for space and nutrients can be intense. This study addressed the hypothesis that secondary metabolites produced by the sediment-inhabiting actinomycete Salinispora arenicola affect community composition and thus mediate interactions among competing microbes. Next-generation amplicon sequencing of mesocosm experiments revealed complex communities that shifted following exposure to S. arenicola extracts. The results reveal that certain predatory bacteria were consistently less abundant following exposure to extracts, suggesting that microbial metabolites mediate competitive interactions. Other taxa increased in relative abundance, suggesting a benefit from the extracts themselves or the resulting changes in the community. This study takes a first step toward assessing the impacts of bacterial metabolites on sediment microbial communities. The results provide insight into how low-abundance organisms may help structure microbial communities in ocean sediments.KEYWORDS chemical ecology, marine sediments, microbial communities, secondary metabolites

show abstract

Bdellovibrio bacteriovorus directly attacks Pseudomonas aeruginosa and Staphylococcus aureus Cystic fibrosis isolates

Cited by 85 publications

References 50 publications

Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Effects of Actinomycete Secondary Metabolites on Sediment Microbial Communities

Contact Info

Product

Resources

About