Bear dentine aspartic acid racemization analysis: a proxy for the dating of Pleistocene cave infills

Torres, Trinidad de; Ortíz, José E.; Llamas, Félix; Canoira, Laureano; Julià, Ramón; Garcia-Martinez, M. J.

doi:10.1111/1475-4754.t01-1-00074

Cited by 24 publications

(26 citation statements)

References 15 publications

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“…The high coefficients of variation shown by archaeological sites and tissues (Table 2) are similar to those reported for historical (Carolan et al 1997;Gilbert et al 2005;Kolman and Tuross 2000) and pre-historical human remains (Serre et al 2004), and cave bears (Ursus deningeri/spelaeus) (Torres et al 2002).…”

Section: Differences In D/l Asp Ratios Among and Inside Archaeologicasupporting

confidence: 81%

“…The detection was done by a NPD detector at 300ºC and the PEAK96 HP software was used to integrate the peak areas. The induced Asp racemization background of the procedure used was estimated in 0.04-0.05, much lower than the actual values measured on recent dentine samples (Torres et al 2002).…”

Section: Amino Acid Racemizationmentioning

confidence: 61%

“…Some factors, such as pH, ionic strength, metal ions presence and water content, have a direct effect on racemization rates (Bada 1972). The variability in racemization ratios in samples from the same site has been interpreted as a consequence of the existence of individual differences in age at death (Sinibaldi et al 1999), differences in response to the extraction procedure (Carolan et al 1997), and local differences in time of formation of cave layers (Torres et al 2002).…”

Section: Previous Research On Modern Samples Have Reported That Variomentioning

confidence: 99%

“…Environmental conditions inside an archaeological site are not homogeneous (Torres et al 2002), so depending on its location a fossil will undergo variable temperature, pH, water lodging, sediment composition, etc (Reiche et al 2003). Taking this into account, the preservation state of the remains will be a combination of its location in a site and the aforementioned differences in fossil ultra-structure.…”

Section: Differences In D/l Asp Ratios Among and Inside Archaeologicamentioning

confidence: 99%

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Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Fernández

Ortíz

Torres

et al. 2008

Forensic Science International: Genetics Supplement Series

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The extent of racemization of aspartic acid (Asp) -expressed as D/L ratio-has been used as a marker of biomolecular degradation in ancient remains. However, Asp racemization rate is highly variable, and depends on biochemical and geochemical factors. In this paper we aim to determine to which extent the fraction analyzed and the Results show significant differences between the D/L Asp ratios obtained in dentine and whole tooth fractions from the same tooth and individual, as well as among dentine samples from the same archaeological site and among samples of the same age from different, though nearby, archaeological sites. Ancient DNA (aDNA) could be characterized in the majority of the samples, independently from their racemization values, which suggests that other factors, apart from the sample preservation stage, are more significantly affecting the racemization rate. A generalized use of the Asp racemization ratio as a threshold value for ancient DNA preservation needs to be questioned until further methodological standardization is considered.

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Section: Differences In D/l Asp Ratios Among and Inside Archaeologicasupporting

confidence: 81%

Section: Amino Acid Racemizationmentioning

confidence: 61%

Section: Previous Research On Modern Samples Have Reported That Variomentioning

confidence: 99%

Section: Differences In D/l Asp Ratios Among and Inside Archaeologicamentioning

confidence: 99%

See 2 more Smart Citations

Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Fernández

Ortíz

Torres

et al. 2008

Forensic Science International: Genetics Supplement Series

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“…Posteriormente, las muestras se dializaron con el fin de eliminar los aminoácidos libres que podrían interferir en los resultados analíticos (cf. Torres et al, 2002).…”

Section: Metodología Y Resultadosunclassified

Geocronología de la Terraza Compleja de Arganda en el valle del río Jarama (Madrid, España)

et al. 2011

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RESUMENLa Terraza Compleja de Arganda (TCA), situada en el tramo bajo del río Jarama (Madrid), está formada por sucesivos apilamientos de secuencias fluviales denominados de abajo a arriba Arganda I, II, III y IV, en los que se han encontrado importantes yacimientos arqueológicos y paleontológicos del Pleistoceno (Áridos 1 y 2, Valdocarros o HAT), y numerosos conjuntos de industria lítica del Paleolítico inferior y medio. Hasta ahora, la única referencia cronológica disponible para la TCA era la proporcionada por el estadio evolutivo de los micromamíferos de los yacimientos Áridos 1 en Arganda I y Valdocarros en Arganda II. En este trabajo, se propone la equivalencia de las distintas unidades de la TCA con terrazas escalonadas y se establece un marco cronológico numérico, obtenido mediante dataciones de termoluminiscencia, luminiscencia ópticamente estimulada y racemización de aminoácidos. Arganda I (≈ T+30-32 m) se situaría hacia el final del MIS 11 o en el inicio del MIS 9, Arganda II (≈T+23-24 m) se correspondería con el inicio del MIS 7, Arganda III (≈T+18-20 m) se situaría entre el MIS 7 y el MIS 5, y Arganda IV comenzaría su deposición en el MIS 5 finalizando su sedimentación en el MIS 1 al sur de Arganda del Rey (Madrid).Palabras clave: Valle del Jarama, Terraza Compleja de Arganda, Pleistoceno, Madrid, luminiscencia, racemización de aminoácidos, bioestratigrafía. ABSTRACTThe Arganda Complex Terrace (TCA) is located in the low valley of Jarama river (Madrid), and is formed by successive pilling up of alluvial deposits identified as Arganda I, II, III and IV, from bottom to top. In these units important archaeological and paleontological sites of Pleistocene age (Áridos 1 and 2, Valdocarros or HAT) have been discovered, as well as large assemblages of lithic industry of the Lower and Middle Palaeolithic. The only chronological available reference for TCA was provided for the evolved state of the micromammals from the sites of Aridos 1 and Valdocarros, located in the lithostratigraphic units of Arganda I and II respectively. In this paper, we suggest the equivalence of the different units of the TCA with the stepped terraces and we state a numerical time-frame obtained by luminiscence dates and amino acid racemization. Arganda I (≈ T+30-32 m) may be correlated to final MIS 11 or beginning of MIS 9, Arganda II (≈T+23-24 m) belongs to start of MIS 7, Arganda III (≈T+18-20 m) may be situated between MIS 7 and MIS 5, and finally Arganda IV begins its deposition in MIS 5 and concludes in MIS 1 to south of Arganda del Rey (Madrid).

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Direct dating of human fossils

Grün

2006

Am. J. Phys. Anthropol.

125

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Bear dentine aspartic acid racemization analysis: a proxy for the dating of Pleistocene cave infills

Cited by 24 publications

References 15 publications

Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Geocronología de la Terraza Compleja de Arganda en el valle del río Jarama (Madrid, España)

Direct dating of human fossils

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