Beclin 1 Interacts With Active Caspase-3 and Bax in Oocytes From Atretic Follicles in the Rat Ovary

Escobar, María Luisa; Echeverría, Olga M.; Martínez, Sebastián Palacios; Juárez-Chavero, Silvia; Sánchez-Sánchez, Luis; Vázquez‐Nin, Gerardo H.

doi:10.1369/0022155419881127

Cited by 19 publications

(13 citation statements)

References 44 publications

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“…Similarly, in a recent report [108], it was demonstrated that Bcl-2, by inhibiting Beclin 1, could result in the release of pro-apoptotic proteins i.e., Bak and Bax to promote apoptosis via activation of effector caspase-3 in atretic follicles in rat ovaries. It was also speculated that this interaction between Beclin 1 and Bcl-2 (or perhaps other Bcl-2 family members) could impair the ability of Bcl-2 to inhibit the pro-apoptotic proteins i.e., Bax and Bak, which in turn, might trigger cell apoptosis [108]. Recently it was also shown that Se deficiency led to an increased mRNA and protein expression of Bcl-2 and inhibited autophagy and induced apoptosis in chicken cardiomyocytes by inhibiting Bax/Bcl-2 and caspases-mediated cleavage of Beclin 1 [111].…”

Section: Expression Of Gpx1 Gpx3 Gpx4 Selenof Bcl-2 and P21mentioning

confidence: 64%

“…It has been shown that Bcl-2 can inhibit the autophagy via its strong independent interaction with Beclin 1 in a highly regulated manner in yeast and mammalian cell lines [107]. Similarly, in a recent report [108], it was demonstrated that Bcl-2, by inhibiting Beclin 1, could result in the release of pro-apoptotic proteins i.e., Bak and Bax to promote apoptosis via activation of effector caspase-3 in atretic follicles in rat ovaries. It was also speculated that this interaction between Beclin 1 and Bcl-2 (or perhaps other Bcl-2 family members) could impair the ability of Bcl-2 to inhibit the pro-apoptotic proteins i.e., Bax and Bak, which in turn, might trigger cell apoptosis [108].…”

Section: Expression Of Gpx1 Gpx3 Gpx4 Selenof Bcl-2 and P21mentioning

confidence: 81%

“…In general, the Bcl-2 family has been implicated in regulating the complex interactions of several anti-apoptotic and pro-apoptotic molecules, governing the apoptosis-activation threshold at cellular levels [106,107]. On the contrary, previous reports have shown that Bcl-2 can also modulate the process of autophagy [107,108]. Although autophagy is generally considered as a pro-survival mechanism [109], at non-physiological levels and in response to different cellular stresses it can lead to cell death [107].…”

Section: Expression Of Gpx1 Gpx3 Gpx4 Selenof Bcl-2 and P21mentioning

confidence: 99%

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Dietary Selenium Supplementation Ameliorates Female Reproductive Efficiency in Aging Mice

et al. 2019

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Female reproductive (ovarian) aging is distinctively characterized by a markedly reduced reproductive function due to a remarkable decline in quality and quantity of follicles and oocytes. Selenium (Se) has been implicated in playing many important biological roles in male fertility and reproduction; however, its potential roles in female reproduction, particularly in aging subjects, remain poorly elucidated. Therefore, in the current study we used a murine model of female reproductive aging and elucidated how different Se-levels might affect the reproductive efficiency in aging females. Our results showed that at the end of an 8-week dietary trial, whole-blood Se concentration and blood total antioxidant capacity (TAOC) were significantly reduced in Se-deficient (0.08 mg Se/kg; Se-D) mice, whereas both of these biomarkers were significantly higher in inorganic (0.33 mg/kg; ISe-S) and organic (0.33 mg/kg; OSe-S) Se-supplemented groups. Similarly, compared to the Se-D group, Se supplementation significantly ameliorated the maintenance of follicles and reduced the rate of apoptosis in ovaries. Meanwhile, the rate of in vitro-produced embryos resulting from germinal vesicle (GV) oocytes was also significantly improved in Se-supplemented (ISe-S and OSe-S) groups compared to the Se-D mice, in which none of the embryos developed to the hatched blastocyst stage. RT-qPCR results revealed that mRNA expression of Gpx1, Gpx3, Gpx4, Selenof, p21, and Bcl-2 genes in ovaries of aging mice was differentially modulated by dietary Se levels. A considerably higher mRNA expression of Gpx1, Gpx3, Gpx4, and Selenof was observed in Se-supplemented groups compared to the Se-D group. Similarly, mRNA expression of Bcl-2 and p21 was significantly lower in Se-supplemented groups. Immunohistochemical assay also revealed a significantly higher expression of GPX4 in Se-supplemented mice. Our results reasonably indicate that Se deficiency (or marginal levels) can negatively impact the fertility and reproduction in females, particularly those of an advancing age, and that the Se supplementation (inorganic and organic) can substantiate ovarian function and overall reproductive efficiency in aging females.

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Section: Expression Of Gpx1 Gpx3 Gpx4 Selenof Bcl-2 and P21mentioning

confidence: 64%

Section: Expression Of Gpx1 Gpx3 Gpx4 Selenof Bcl-2 and P21mentioning

confidence: 81%

Section: Expression Of Gpx1 Gpx3 Gpx4 Selenof Bcl-2 and P21mentioning

confidence: 99%

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Dietary Selenium Supplementation Ameliorates Female Reproductive Efficiency in Aging Mice

et al. 2019

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“…It has been documented that this apoptotic pathway is responsible for the expression of Bcl‐2 family members, the loss of mitochondrial transmembrane potential (MMP) and the release of cytochrome c from mitochondria to the cytoplasm 34 . Triggering the cascade reaction of the caspase family and activating caspase‐3 and finally triggering apoptosis 35 . The Bcl‐2 protein family includes pro‐apoptotic and anti‐apoptotic members, such as Bax and Bcl‐2.…”

Section: Discussionmentioning

confidence: 99%

The comparison analysis of polyphyllin I and its analogues induced apoptosis of colon and lung cancer cells via mitochondrial dysfunction

You

Geng

Yang

et al. 2021

Basic Clin Pharma Tox

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Polyphyllin I (PPI) and its analogues, including polyphyllin II (PPII), polyphyllin VI (PPVI) and polyphyllin VII (PPVII), are major bioactive compounds isolated from the Chinese herb Chonglou. However, the susceptibilities of PPI and its analogues towards the different cell lines are diversified and the mechanisms are not fully clarified. Thus, the present study aimed to investigate the cytotoxicity of PPI and its analogues on two different cell lines, as well as to explore the underlying mechanisms of these agents via inducing mitochondrial dysfunction. The results showed that PPI and its analogues were cytotoxic agents towards both A549 and HT‐29 cells, with IC50 values ranged from 1.0 to 4.5 μmol/L. Further investigations demonstrated that they decreased the mitochondrial membrane potentials of both A549 and HT‐29 cells in a dose‐dependent manner. Among all tested compounds, PPVI and PPI induced the most obvious changes in Ca2+ haemostasis in these two cell lines. In addition, they could induce the accumulation of ROS in cells and down‐regulated the Bcl‐2 expression, up‐regulated the Bax expression and induced the activity of cleaved caspase‐3 in cells. Collectively, our findings clearly demonstrated the cytotoxic differences and mechanisms of PPI and its analogues induced cell apoptosis and could partially explain the anticancer effects of these natural constituents in Chonglou.

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“…CHOP can trigger the intrinsic apoptotic pathway through the inhibition of BCL2, BCL-XL, and MCL-1 and the upregulation of BIM, which regulates BAX-BAK-mediated mitochondrial outer membrane permeabilization, leading to Cyto C release and the Caspase cascade ( Hu et al, 2018 ; Kim and Kim, 2018 ). Previous studies have identified that the expression of key regulators of oocyte apoptosis (Bax and Caspase 3) is increased in mouse oocyte apoptosis ( Escobar et al, 2019 ; Wang F. et al, 2020 ; Wang L. et al, 2020 ). Overexpression of Bax modulates the mitochondrial ΔΨ m and triggers Cyto C release and induces the activation of Caspase 3, leading to DNA fragmentation and thereby oocyte apoptosis ( Chaube et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes

Gao

Zhang

et al. 2021

Front. Cell Dev. Biol.

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The endoplasmic reticulum (ER) is a multifunctional organelle in the cytoplasm that plays important roles in female mammalian reproduction. The endoplasmic reticulum and mitochondria interact to maintain the normal function of cells by maintaining intracellular calcium homeostasis. As proven by previous research, glycine (Gly) can regulate the intracellular free calcium concentration ([Ca2+]i) and enhance mitochondrial function to improve oocyte maturation in vitro. The effect of Gly on ER function during oocyte in vitro maturation (IVM) is not clear. In this study, we induced an ER stress model with thapsigargin (TG) to explore whether Gly can reverse the ER stress induced by TG treatment and whether it is associated with calcium regulation. The results showed that the addition of Gly could improve the decrease in the average cumulus diameter, the first polar body excretion rate caused by TG-induced ER stress, the cleavage rate and the blastocyst rate. Gly supplementation could reduce the ER stress induced by TG by significantly improving the ER levels and significantly downregulating the expression of genes related to ER stress (Xbp1, ATF4, and ATF6). Moreover, Gly also significantly alleviated the increase in reactive oxygen species (ROS) levels and the decrease in mitochondrial membrane potential (ΔΨ m) to improve mitochondrial function in porcine oocytes exposed to TG. Furthermore, Gly reduced the [Ca2+]i and mitochondrial Ca2+ ([Ca2+]m) levels and restored the ER Ca2+ ([Ca2+]ER) levels in TG-exposed porcine oocytes. Moreover, we found that the increase in [Ca2+]i may be caused by changes in the distribution and expression of inositol 1,4,5-triphosphate receptor (IP3R1) and voltage-dependent anion channel 1 (VDAC1), while Gly can restore the distribution and expression of IP3R1 and VDAC1 to normal levels. Apoptosis-related indexes (Caspase 3 activity and Annexin-V) and gene expression Bax, Cyto C, and Caspase 3) were significantly increased in the TG group, but they could be restored by adding Gly. Our results suggest that Gly can ameliorate ER stress and apoptosis in TG-exposed porcine oocytes and can further enhance the developmental potential of porcine oocytes in vitro.

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Beclin 1 Interacts With Active Caspase-3 and Bax in Oocytes From Atretic Follicles in the Rat Ovary

Cited by 19 publications

References 44 publications

Dietary Selenium Supplementation Ameliorates Female Reproductive Efficiency in Aging Mice

Dietary Selenium Supplementation Ameliorates Female Reproductive Efficiency in Aging Mice

The comparison analysis of polyphyllin I and its analogues induced apoptosis of colon and lung cancer cells via mitochondrial dysfunction

Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes

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