Beef donor cows with high number of retrieved COC produce more in vitro embryos compared with cows with low number of COC after repeated ovum pick-up sessions

Monteiro, Fábio Morato; Batista, E. O. S.; Vieira, Leandro M.; Bayeux, Bernardo Marcozzi; Accorsi, M. F.; Campanholi, Suzane Peres; Dias, E. A. R.; Souza, A. H.; Baruselli, Pietro Sampaio

doi:10.1016/j.theriogenology.2016.11.002

Cited by 32 publications

(34 citation statements)

References 18 publications

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“…Similarly, to what occurs in the production of embryo in vivo (by SOV), potential oocyte donors can be selected based on the ovarian antral follicle count (Boni et al, 1997). Different studies demonstrated that donors with greater COC yield are those that produces more embryos and, consequently, a greater number of pregnancies (Pontes et al, 2010;Monteiro et al, 2017). Interestingly, pregnancy rate does not seem to be affected by ovarian follicle population (Feres et al, 2016).…”

Section: Ultrasonography and The Development Of Assisted Reproductivementioning

confidence: 99%

Current Status, Emerging Technologies, and Trends in Veterinary Ultrasonography Applied to Cattle Reproduction

Viana¹,

Siqueira²

2017

SPERMOVA

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The B-mode and color Doppler ultrasonography provide a safe, non-invasive, real-time approach to assess reproductive tissues and organs in female cattle, and thus have a number of potential uses in reproductive management. On the ovary, antral follicle population can be counted, and follicular growth, regression and ovulation can be monitored during sequential exams. The corpus luteum can be identified, and evaluated according to size, echotexture, and vascularization. Ultrasonography allows an early diagnosis of pregnancy based in the detection of the embryonic vesicle from day 24 of gestation. Alternatively, non-pregnancy can be indirectly inferred according to corpus luteum vascularization 20 days after insemination. Embryo and fetus morphology and vital signs can monitored throughout gestation, and fetal sex can be determined by the visualization of the relative position of the genital tubercle. In addition, a range of pathologic conditions of the ovary and uterus can be diagnosed based on sonographic images. The use of ultrasonography was also key for the development of many assisted reproductive technologies, such as timed artificial insemination, modern superovulation protocols, procedures for the recovery of cumulus-oocyte complexes aiming in vitro embryo production, and is a valuable tool for the selection and management of embryo recipients. In the future, emerging technologies such as computer-assisted image analysis, 3D ultrasonography, and 3D modeling have the potential to improve the accuracy of sonographic diagnosis, or even create new potential uses for ultrasonography in animal reproduction.Keywords: 3D ultrasound, bovine, color Doppler, pregnancy diagnosis, reproductive biotechnologies RESUMEN La ecografía de modo B y dopler color proporciona un enfoque seguro, no invasivo y en tiempo real para evaluar tejidos y órganos reproductivos en vacas, por lo tanto, tiene una serie de usos potenciales en el manejo reproductivo. En el ovario, se puede realizar el conteo de la población de folículos antrales, y realizar el seguimiento del crecimiento folicular, la regresión y la ovulación durante monitoreo secuencial. El cuerpo lúteo puede ser identificado y evaluarse según el tamaño, ecotextura y vascularización. La ecografía permite un diagnóstico precoz de la preñez basado en la detección de la vesícula embrionaria desde el día 24 de la gestación. Alternativamente, la ño preñez puede inferirse indirectamente de acuerdo con la vascularización del cuerpo lúteo a los 20 días después de la inseminación. La morfología y los signos vitales del embrión y del feto pueden monitorearse durante toda la gestación, y el sexo del feto puede determinarse mediante la visualización de la posición relativa del tubérculo genital. Además, se puede diagnosticar una variedad de condiciones patológicas del ovario y el útero según las imágenes ecográficas. El uso de ultrasonografía también fue clave para el desarrollo de muchas tecnologías de reproducción asistida, como la inseminación artificial a tiempo fijo, los pr...

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Section: Ultrasonography and The Development Of Assisted Reproductivementioning

confidence: 99%

Current Status, Emerging Technologies, and Trends in Veterinary Ultrasonography Applied to Cattle Reproduction

Viana¹,

Siqueira²

2017

SPERMOVA

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“…Dicha respuesta es atribuible principalmente al efecto promotor del reclutamiento y el crecimiento de los folículos por parte de la FSH, dado que es ampliamente aceptado el papel de la FSH en la folículogénesis, lo cual implica que la variación en el número de folículos en crecimiento durante una onda folicular estaría positivamente asociada al menos durante una parte de la onda, con las concentraciones séricas de FSH (Ireland et al, 2007;Vieira et al, 2014). Recientemente se plantea la definición de las hembras bovinas como de bajo potencial, cuando producen un número limitado de embriones a causa de su limitada población de folículos antráles pequeños presentes en el ovario al momento de iniciar el tratamiento con FSH (Durocher et al 2006;Monteiro et al, 2017). Se sugiere que el crecimiento acelerado de los folículos ováricos por la acción de la FSH, altera el ambiente folicular idóneo en donde normalmente los oocitos alcanzan un estado de competencia para el desarrollo (Monteiro et al, 2017;Qui et al, 2013;Ward et al, 2002).…”

Section: ■ Discusiónunclassified

“…Recientemente se plantea la definición de las hembras bovinas como de bajo potencial, cuando producen un número limitado de embriones a causa de su limitada población de folículos antráles pequeños presentes en el ovario al momento de iniciar el tratamiento con FSH (Durocher et al 2006;Monteiro et al, 2017). Se sugiere que el crecimiento acelerado de los folículos ováricos por la acción de la FSH, altera el ambiente folicular idóneo en donde normalmente los oocitos alcanzan un estado de competencia para el desarrollo (Monteiro et al, 2017;Qui et al, 2013;Ward et al, 2002). De manera que la maduración del oocito no es directamente proporcional a la maduración folicular, con lo cual sería posible esperar una alteración en la calidad intrínseca del oocito establecida por parámetros morfológicos.…”

Section: ■ Discusiónunclassified

Evaluación de la estimulación ovárica y la calidad de oocitos bovinos obtenidos por aspiración folicular

Giraldo¹,

Ramírez²,

Oquendo³

et al. 2017

J. agric. anim. sci.

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Introducción. La aspiración folicular guiada por ultrasonido (AFGU) acoplada a la producción in vitro de embriones (PIVE), es una alternativa importante para el aprovechamiento de los recursos genéticos de hembras bovinas de alto potencial productivo. Sin embargo, la calidad reducida de los oocitos recuperados por esta técnica, es uno de los aspectos que más afecta la eficiencia de obtención de embriones. Materiales y Métodos. Esta investigación comparó el desarrollo folicular, y la calidad de los oocitos recuperados por AFGU, en hembras bovinas superestimuladas convencionalmente con FSH, o con FSH y hCG. Resultados. Se evidenció una mayor calidad en los oocitos recuperados de animales estimulados con FSH y hCG, respecto a aquellos estimulados solo con FSH. Conclusiones. La inclusión de hCG en un esquema para la superestimulación ovárica con FSH de hembras bovinas posteriormente sometidas a aspiración folicular, ejerce un efecto favorable sobre la calidad de oocitos recuperados.Palabras claves: estimulación ovárica, folículos ováricos, gonadotropinas. Introduction. Ultrasound-guided follicular aspiration (UGFA) coupled with the in-vitro production of embryos is an important alternative for the use of genetic resources of bovine females of high productive potential. However, the reduced quality of oocytes recovered with this technique is one of the aspects with the highest impact on the efficiency to obtain embryos. Materials and methods. This research compared follicular development with the quality of oocytes recovered by UGFA, in bovine females conventionally over-stimulated with FSH or FSH and hCG. Results. A higher quality of oocytes recovered from animals stimulated with FSH and hCG was seen compared to the ones stimulated with FSH only. Conclusions. Inclusion of Hcg in a scheme for the ovarian over-stimulation with FSH of bovine females subject to follicular aspiration becomes a favorable effect on the quality of recovered oocytes.

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“…The recovery of oocytes via follicular aspiration associated with in vitro fertilization is a biotechnique used to accelerate the production of animals with superior characteristics. Embryos produced in vitro correspond to more than 40% of the embryos produced in the world (Monteiro et al, 2017;Perry, 2014). This interest in the in vitro fertilization is due to several factors, such as improvements in the in vitro culture system, acceleration of the production of animals with superior characteristics, and the possibility of using sexed semen, enabling the production of livestock of a defined sex (Gimenes et al, 2015;Monteiro et al, 2017;Pontes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Despite advances in in vitro fertilization, several factors may still limit the achievement of success of this biotechnology, such as: variations in the number of ovarian follicles between animals or breeds (Bos indicus and Bos taurus), and the management techniques for in vivo production of embryos (Baruselli et al, 2007;Gimenes et al, 2015;Monteiro et al, 2017;Pontes et al, 2010). There is still no conclusive biological explanation to explain the variability between animals in the amount of oocytes aspirated via ovum pickup (OPU) (Pontes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

Moraes¹,

Adona²,

Guemra³

et al. 2019

Braz. J. Vet. Res. Anim. Sci.

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The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage. Keywords: Bovine. Follicle. Ovum pickup. RESUMOO presente estudo avaliou vacas Brangus tratadas com doses únicas de hormônio folículo estimulante (FSH) submetidas a aspiração folicular após vinte e quatro horas, para avaliação da recuperação oocitária, fertilização in vitro e taxa de prenhez. Folículos superiores a três milímetros de diâmetro foram aspirados, 200 mg de FSH foram administrados dois dias depois e uma nova aspiração folicular foi realizada 24 horas após. Esses métodos foram efetivados três vezes a cada três dias. No controle, as aspirações foliculares ocorreram em intervalos de uma semana sem administração de FSH. Os oócitos aspirados foram avaliados, submetidos à fertilização in vitro e os embriões foram transferidos em receptoras. A recuperação média dos oócitos foi superior (p<0,05) nas vacas controle (12,4±1,8) do que nas vacas tratadas (9,4±1,3). Não houve diferença (p>0,05) na porcentagem média de oócitos viáveis (52,0±3,9 e 62,7±4,7%) ou na porcentagem média de embriões (41,4±4,8 e 41,5±4,2%) entre vacas controle e vacas tratadas, respectivamente. A porcentagem média de prenhez não diferiu (p>0,05) para as vacas controle (43,8±2,7%) e as tratadas (40,9±6,8%). Em conclusão, o tratamento com FSH não melhorou a recuperação de oócitos, a fertilização in vitro e o percentual de prenhez. No entanto, existe a possibilidade de várias aspirações foliculares consecutivas a cada três dias, concentrando a fertilização in vitro e o percentual de prenhez. Palavras-chave: Aspiração follicular. Bovinos. Folículos. 2/8 Braz J Vet Res Anim Sci. 2019;56(3):e156894 How to cite: Moraes MEB, Adona PR, Guemra S, De Bem THC, Miranda MS. Effect of single dose follicle stimulating hormone on fol...

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Beef donor cows with high number of retrieved COC produce more in vitro embryos compared with cows with low number of COC after repeated ovum pick-up sessions

Cited by 32 publications

References 18 publications

Current Status, Emerging Technologies, and Trends in Veterinary Ultrasonography Applied to Cattle Reproduction

Current Status, Emerging Technologies, and Trends in Veterinary Ultrasonography Applied to Cattle Reproduction

Evaluación de la estimulación ovárica y la calidad de oocitos bovinos obtenidos por aspiración folicular

Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

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