Beef specific polymerase chain reaction assay for authentication of meat and meat products

Mane, B. G.; Mendiratta, S. K.; Tiwari, Amit Kumar

doi:10.1016/j.foodcont.2012.05.031

Cited by 42 publications

(32 citation statements)

References 19 publications

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“…In recent years, awareness of food safety and quality has increased, and issues such as the adulteration of meat products have become critical in matters involving personal health, economy and religion (MANE et al, 2012a).Although, food adulteration has been reported by various researchers worldwide (MANE et al, 2012b;KARABASANAVARet al, 2013;MOUSAVI et al, 2015), few scientific studies have investigated the frequency of food adulteration in different Brazilian states. Furthermore, fast methods that may be routinely used to detect food adulteration are seldom described.…”

Section: Introductionmentioning

confidence: 99%

Brazilian ground beef authentication by multiplex polymerase chain reaction

Oliveira

Pedroso²,

Cardilli³

et al. 2018

Cienc. Rural

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The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.

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Section: Introductionmentioning

confidence: 99%

Brazilian ground beef authentication by multiplex polymerase chain reaction

Oliveira

Pedroso²,

Cardilli³

et al. 2018

Cienc. Rural

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“…Karabasanavar et al (2011b) obtained 0.1% sensitivity for mutton with a 0.001 ng LOD. Mane et al (2012) detected less than 1% adulteration of beef in admixed meat and meat products. Thus, the LOD we obtained in this study was far below those of previously reported assays.…”

Section: Sensitivity Test Under Pure Admixed and Commercial Meatballsmentioning

confidence: 95%

“…In food adulteration studies, beef (Mane et al 2012), chicken (Mane et al 2009), turkey (Rodriguez et al 2003), goat (Karabasanavar et al 2011a), lamb and pork (Rodríguez et al 2004) among the livestock, and deer (Fajardo et al 2008) and wild boar (Mutalib et al 2012) among the wild animals have been extensively studied. However, to the best of our knowledge, no detection technique has been documented or articulated for MBT species detection under complex background and commercial foods.…”

Section: Sensitivity Test Under Pure Admixed and Commercial Meatballsmentioning

confidence: 99%

“…Meat samples were boiled at 100°C for 60, 90, 120 and 150 min, but no adverse effect was found for the 120 bp target, whereas the 165 bp target failed to amplify from 150 min-boiled meat samples ( Figure 4(a)). Previously, Haunshi et al (2009);Karabasanavar et al (2011a) and Mane et al (2012) studied the effect of autoclaving on DNA by treating various types of domestic meat at 121°C for 15-30 min and found their samples were stable at this condition. We further autoclaved MBT meat at 120°C for 60, 90, 120 and 150 min (extensive treatment) and found 120 bp PCR products under all conditions.…”

Section: Effect Of Processing Treatments and Comparison Studymentioning

confidence: 99%

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A suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain

Ali

Asing

et al. 2015

Food Additives & Contaminants: Part A

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Malayan box turtle (Cuora amboinensis) has been a wildlife-protected vulnerable turtle species in Malaysia since 2005. However, because of its purported usage in traditional medicine, tonic foods and feeds, clandestine black market trade is rampant. Several polymerase chain reaction (PCR) assays for the taxonomic detection and classification of turtle species have been proposed. These assays are based on long-length target amplicons which are assumed to break down under compromised states and, hence, might not be suitable for the forensic tracing and tracking of turtle trafficking. For the first time this paper develops a very short-amplicon-length PCR assay (120 bp) for the detection of Malayan box turtle meat in raw, processed and mixed matrices, and experimental evidence is produced that such an assay is not only more stable and reliable but also more sensitive than those previously published. We checked the assay specificity against 20 different species and no cross-species detection was observed. The possibility of any false-negative detection was eliminated by a universal endogenous control for eukaryotes. The assay detection limit was 0.0001 ng of box turtle DNA from pure meat and 0.01% turtle meat in binary and ternary admixtures and commercial meatballs. Superior target stability and sensitivity under extreme treatments of boiling, autoclaving and microwave cooking suggested that this newly developed assay would be suitable for any forensic and/or archaeological identification of Malayan box turtle species, even in severely degraded specimens. Further, in silico studies indicated that the assay has the potential to be used as a universal probe for the detection of nine Cuora species, all of which are critically endangered.

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“…Species-specific PCR amplifies a very small amount of genetic target and has been accepted as conclusive for the detection of chicken (Haunshi et al 2009), goat , beef (Mane et al 2012), pork, and dog (Ali et al 2012d(Ali et al , 2013 in a simplex platform; pork and chicken in duplex PCR (Soares et al 2010); beef, chicken, and pork (Ghovvati et al 2009) in triplex, and cattle, pig, chicken, sheep, goat, and horse (Matsunaga et al 1999) in multiplex PCR systems. Multiplex PCR is theoretically interesting, but optimization of PCR conditions to detect multiple targets is really challenging ).…”

Section: Specificity Testmentioning

confidence: 99%

Short Amplicon-Length PCR Assay Targeting Mitochondrial Cytochrome b Gene for the Detection of Feline Meats in Burger Formulation

et al. 2015

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Consumption or mixing of feline ingredients in halal and kosher foods is forbidden, and various diseases such as SARS, anthrax, and hepatitis could be transmitted through feline meats. However, since feline species are abundant across the world without any market price and their meats are consumed in exotic foods, the chances of their adulteration in common meats are very high. Several recent reports appreciated short amplicon-length PCR assays for species authentication in processed foods assuming that shorter targets would be thermodynamically more stable than longer ones under natural decomposition and food processing treatments. However, scientific evidence to prove this hypothesis is rarely documented. For the first time, we developed here a PCR assay targeting only a 69-bp site of feline mitochondrial cytochrome b gene, and its authenticity was confirmed by AluI restriction enzyme followed by its separation and detection on a lab-on-a-chip-based automated electrophoretic system. The exceptional target stability was systematically proven over the previously documented shortest target (108 bp) under extreme autoclaving and microwaving treatments both in pure and mixed matrices. The assay specificity was tested against 14 terrestrial and aquatic species commonly consumed in foods, and no cross-species detection was observed. The limit of detection of the assay was 0.1 pg of feline DNA and 0.01 % (w/w) of feline meats in raw meats and cooked burgers, respectively.

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Beef specific polymerase chain reaction assay for authentication of meat and meat products

Cited by 42 publications

References 19 publications

Brazilian ground beef authentication by multiplex polymerase chain reaction

Brazilian ground beef authentication by multiplex polymerase chain reaction

A suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain

Short Amplicon-Length PCR Assay Targeting Mitochondrial Cytochrome b Gene for the Detection of Feline Meats in Burger Formulation

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