Beer consumption negatively regulates hormonal reproductive status and reduces apoptosis in Leydig cells in peripubertal rats

Oczkowski, Michał; Rembiszewska, Alina; Dziendzikowska, Katarzyna; Woliñska-Witort, Ewa; Kołota, Aleksandra; Malik, Agnieszka; Stachoń, Małgorzata; Lachowicz, Katarzyna; Gromadzka-Ostrowska, Joanna

doi:10.1016/j.alcohol.2019.01.009

Cited by 6 publications

(5 citation statements)

References 80 publications

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“…Before the main experiment, the weaned 21-day-old rats were housed in group cages (six animals per cage) and allowed to adapt to conditions at the vivarium for 10 days. During this period, animals from the experimental groups received solutions of alcoholic beverages (2%, 4%, 6%, 8%, and 10% of ethanol solutions, increased every second day) every day and had free access to water, as described in a previously published study [19]. At the end of the adaptation period, the 30-day-old rats underwent the experimental procedure.…”

Section: Methodsmentioning

confidence: 99%

Influence of Alcohol Consumption on Body Mass Gain and Liver Antioxidant Defense in Adolescent Growing Male Rats

Kołota

Głąbska

Oczkowski

et al. 2019

IJERPH

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The World Health Organization (WHO) reported that alcohol consumption is a serious problem in adolescents. The aim of the study was to assess the influence of the time of exposure of various alcoholic beverages on body mass as well as on select parameters of liver antioxidant defense in adolescent Wistar rats. Thirty-day-old animals were divided into 12 groups (six animals in each): control and groups receiving various beverages containing 10% of alcohol (ethanol, red wine, beer), observed for two, four, and six weeks. The body weight gain and energy supply were analyzed for body mass assessment. The catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase, transferase (GST), reductase activities, total antioxidant status, and glutathione level (GSH) were analyzed, for a liver antioxidant defense assessment. Group receiving red wine was characterized by the highest alcohol intake, lowest dietary intake, and highest total energy supply (p < 0.05). However, this did not influence body weight gain (p > 0.05). Reduced diet intake in groups receiving alcohol was counterbalanced by its energy value. Therefore, the energy supply was not lower than for the control (p > 0.05). Alcohol consumption and the experiment duration influenced CAT, SOD, and GST activities and GSH level. Alcohol consumption may influence hepatic antioxidant defense in adolescent male rats, but without influence on body weight gain.

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Section: Methodsmentioning

confidence: 99%

Influence of Alcohol Consumption on Body Mass Gain and Liver Antioxidant Defense in Adolescent Growing Male Rats

Kołota

Głąbska

Oczkowski

et al. 2019

IJERPH

Self Cite

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“…Immunolocalization of steroid receptors and aromatase in Leydig cells (LCs) was performed on 6 µm thick sections. A detailed description of the method has been published previously by our team [20]. Briefly, sections were washed in PBS (pH = 7.4) and incubated overnight at 4 • C with polyclonal anti-rabbit primary antibodies (catalog numbers: sc-816 from Santa Cruz Biotechnology, Paso Robles, CA, USA, for AR; NCL-L_ER-6F11 from Novocastra, Newcastle, UK, for ERα; 07-359 from Millipore, Darmstadt, Germany, for ERβ and MCA2077S from AbD Serotec, Oxford, UK, for Aro).…”

Section: Immunohistological Evaluation Of Estrogen Receptors (Erα Erβ...mentioning

confidence: 99%

Does Nanosilver Exposure Modulate Steroid Metabolism in the Testes?—A Possible Role of Redox Balance Disruption

Oczkowski,

Dziendzikowska,

Gromadzka-Ostrowska

et al. 2023

Biomedicines

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Silver nanoparticles (AgNPs) are a popular engineered nanomaterial widely used in industry. Despite the benefits they bring to society, AgNPs are not neutral to human health. The aim of this study was to evaluate the effects of a single intravenous dose (5 mg/kg body weight) of 20 nm AgNPs on steroid metabolism and redox balance in the testes of adult rats. The effects were evaluated 1 day or 28 days after intervention and compared with saline-treated animals. Decreased aromatase and estrogen receptor α levels (by 21% and 27%, respectively) were observed 1 day after AgNPs administration, while increased testosterone, increased dihydrotestosterone levels, higher androgen receptors and higher aromatase expression in Leydig cells (by 43%, 50%, 20% and 32%, respectively) as well as lower (by 35%) androgen receptor protein levels were observed 28 days after exposure to AgNPs compared to control groups. The AgNPs treatment resulted in decreased superoxide dismutase activity, decreased GSH/GSSG ratio, and increased glutathione reductase activity (by 23%, 63% and 28%, respectively) compared to control animals, irrespective of the time of measurement. Increased (by 28%) intratesticular lipid hydroperoxides level was observed 1 day after AgNPs exposure, while decreased (by 70%) GSH and increased (by 43%) 7-ketocholesterol levels were observed 28 days after treatment compared to control animals. Conclusions: AgNPs exposure caused redox imbalance in the gonads shortly after AgNPs administration, while a longer perspective AgNPs exposure was associated with impaired androgen metabolism, probably due to increased oxidative stress.

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“…It is noteworthy that the authors could identify the involvement of extracellular signal-regulated kinase1/2, phosphatidylinositol 3-kinase/protein kinase B and VEGF receptor 2 in the protective properties of beer towards pulmonary arterial hypertension [ 93 ]. In a prepubertal rat model, beer with 10% alcohol significantly decreased, after 4 weeks, the levels of sex hormones, compared to ethanol- or water-fed rats [ 92 ]. Again, even if authors concluded that beer inhibited the ethanol-induced increase of cleaved caspase-3 in Leydig cells, a non-alcoholic beer was not tested.…”

Section: Phenols-related Health Effects Of Beer Consumptionmentioning

confidence: 99%

The Role of Bioactive Phenolic Compounds on the Impact of Beer on Health

2021

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This review reports recent knowledge on the role of ingredients (barley, hop and yeasts), including genetic factors, on the final yield of phenolic compounds in beer, and how these molecules generally affect resulting beer attributes, focusing mainly on new attempts at the enrichment of beer phenols, with fruits or cereals other than barley. An entire section is dedicated to health-related effects, analyzing the degree up to which studies, investigating phenols-related health effects of beer, have appropriately considered the contribution of alcohol (pure or spirits) intake. For such purpose, we searched Scopus.com for any kind of experimental model (in vitro, animal, human observational or intervention) using beer and considering phenols. Overall, data reported so far support the existence of the somehow additive or synergistic effects of phenols and ethanol present in beer. However, findings are inconclusive and thus deserve further animal and human studies.

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Beer consumption negatively regulates hormonal reproductive status and reduces apoptosis in Leydig cells in peripubertal rats

Cited by 6 publications

References 80 publications

Influence of Alcohol Consumption on Body Mass Gain and Liver Antioxidant Defense in Adolescent Growing Male Rats

Influence of Alcohol Consumption on Body Mass Gain and Liver Antioxidant Defense in Adolescent Growing Male Rats

Does Nanosilver Exposure Modulate Steroid Metabolism in the Testes?—A Possible Role of Redox Balance Disruption

The Role of Bioactive Phenolic Compounds on the Impact of Beer on Health

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