Beet Necrotic Yellow Vein Virus and Its Relationship to Eight Sugar Beet Furo-like Viruses from the United States

Wisler, G. C.; Liu, H. Y.; Duffus, J. E.

doi:10.1094/pd-78-0995

Cited by 29 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BSBMV, like BNYVV, is transmitted by P. betae (44) and is a multiparticulate, rodshaped virus. The RNA is polyadenylated, and the number and size of BSBMV RNAs correspond closely to those of BNYVV.…”

Section: Discussionmentioning

confidence: 99%

“…For Northern hybridization, radioactive probes were generated by reamplification of BNYVV polymerase chain reaction (PCR) products (31,35,44). BNYVV probes were made using primers specific for sequences from the 3´ and 5´ region of each RNA species of BNYVV (44).…”

Section: Methodsmentioning

confidence: 99%

“…BNYVV probes were made using primers specific for sequences from the 3´ and 5´ region of each RNA species of BNYVV (44). A 20-µl PCR mix included 5 µl of the PCR product, 50 µCi [α-32 P]dCTP (3,000 Ci/mmole), 0.2 mM each dNTP, 20 pmole primers (32), 1 unit of Taq DNA polymerase (Cetus), and PCR buffer supplied by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

“…This virus was designated Texas 7 (TX7). Wisler et al (43,44) examined relationships between BNYVV and eight other rod-shaped virus isolates from Texas, Nebraska, and Idaho that were serologically indistinguishable from TX7. They coined the name beet soilborne mosaic virus (BSBMV) to identify these isolates and others with similar serological reaction.…”

Section: Characteristics Of Beet Soilborne Mosaic Virusmentioning

confidence: 99%

“…BNYVV and beet soilborne virus (BSBV) are multipartite, rigid, rod-shaped viruses that infect sugar beet and are transmitted, like BSBMV (44), by Polymyxa betae Keskin. BNYVV and BSBV are tentative members of the genus Furovirus (13,30,39).…”

Section: Characteristics Of Beet Soilborne Mosaic Virusmentioning

confidence: 99%

See 4 more Smart Citations

Characteristics of Beet Soilborne Mosaic Virus, a Furo-like Virus Infecting Sugar Beet

et al. 1997

View full text Add to dashboard Cite

Beet soilborne mosaic virus (BSBMV) is a rigid rod-shaped virus transmitted by Polymyxa betae. Particles were 19 nm wide and ranged from 50 to over 400 nm, but no consistent modal lengths could be determined. Nucleic acids extracted from virions were polyadenylated and typically separated into three or four discrete bands of variable size by agarose-formaldehyde gel electrophoresis. RNA 1 and 2, the largest of the RNAs, consistently averaged 6.7 and 4.6 kb, respectively. The sizes and number of smaller RNA species were variable. The molecular mass of the capsid protein of BSBMV was estimated to be 22.5 kDa. In Northern blots, probes specific to the 3′ end of individual beet necrotic yellow vein virus (BNYVV) RNAs 1–4 hybridized strongly with the corresponding BNYVV RNA species and weakly with BSBMV RNAs 1, 2, and 4. Probes specific to the 5′ end of BNYVV RNAs 1–4 hybridized with BNYVV but not with BSBMV. No cross-reaction between BNYVV and BSBMV was detected in Western blots. In greenhouse studies, root weights of BSBMV-infected plants were significantly lower than mock-inoculated controls but greater than root weights from plants infected with BNYVV. Results of serological, hybridization, and virulence experiments indicate that BSBMV is distinct from BNYVV. However, host range, capsid size, and the number, size, and polyadenylation of its RNAs indicate that BSBMV more closely resembles BNYVV than it does other members of the genus Furovirus.

show abstract

Section: Discussionmentioning

confidence: 99%