Beet Yellows Closterovirus: Complete Genome Structure and Identification of a Leader Papain-like Thiol Protease

Agranovsky, Alexey A.; Koonin, Eugene V.; Boyko, Vitaly; Maiß, E.; Frötschl, Roland; Lunina, N.A.; Atabekov, J.G.

doi:10.1006/viro.1994.1034

Cited by 162 publications

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“…Two genera have been proposed. The genus Closterovirus includes those aphid-transmissible viruses with a monopartite genome of up to 20 000 nucleotides (nt), including beet yellows virus (BYV) (Agranovsky et al, 1994), citrus tristeza virus (CTV) and beet yellow stunt virus (BYSV) (Karasev et al, 1996). The other genus, which includes whitefly-transmissible viruses with a bipartite genome, is represented by lettuce infectious yellows virus (LIYV) (Klaassen et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Four closterovirus genomes have been completely sequenced (Agranovsky et al, 1994 ;Karasev et al, 1995 ;Klaassen et al, 1995 ;Jelkmann et al, 1997). Based on the presence of certain common structures in genome organization, Dolja et al (1994) first proposed the division of a closterovirus genome into four modules : the accessory module of proteinase and vector-specificity factor, the core module of three essential elements for virus replication, methyltransferase (MTR), helicase (HEL) and RNA-dependent RNA polymerase (RdRp), the molecular chaperone module of cellular heat shock protein 70 (HSP70) homologue and a distantly related heat shock protein 90 (HSP90) homologue, and the structural module of coat protein (CP) and its duplicate (CPd).…”

Section: Introductionmentioning

confidence: 99%

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Nucleotide sequence of the 3'-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus.

Ling¹,

Zhu²,

Drong³

et al. 1998

Journal of General Virology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence of the 3'-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus.

Ling¹,

Zhu²,

Drong³

et al. 1998

Journal of General Virology

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“…As the anchor ligation method (Keim-Konrad & Jelkmann, 1996) failed to yield PCR products, tailing of the dsRNA with rATP followed by applying a 5h\3h RACE protocol (Boehringer Mannheim) was used. The 5h-terminal residue determined from the minus strand of LChV dsRNA is a C. Genomic RNAs of other closteroviruses start with an A or G (Agranovsky et al, 1994 ;Klaassen et al, 1995 ;Karasev et al, 1995), as is characteristic for the residues next to the 5h-m7G cap in eukaryotic messengers. However, Karasev et al (1995) Local similarity searches were performed using the program ALIGN-2 from the GENEBEE package (Brodsky et al, 1990).…”

mentioning

confidence: 99%

“…Y10237). With respect to its monopartite genome size, LChV is intermediate between BYV (15 480 nt ; Agranovsky et al, 1994) and CTV (19 296 nt ; Karasev et al, 1995), and is the second largest plant virus sequenced to date. As the previous electrophoretic size estimations of the LCD-associated dsRNA of ca.…”

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Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus.

Wilhelm¹,

Fechtner

Agranovsky³

1997

Journal of General Virology

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The 5h-terminal genomic region (8597 nt) of little cherry virus (LChV), a mealybug-borne closterovirus, was cloned from double-stranded RNA, and its sequence determined to complete the 16 934 nt sequence of the monopartite LChV RNA genome. In the 5h to 3h direction, the sequence encompasses ORF 1a, encoding the conserved replicative domains of methyltransferase and helicase, and ORF 1b, encoding RNA polymerase. ORFs 1a and 1b partially overlap (in 0/M 1 configuration), and the LChV replicase is probably expressed by ribosomal frameshifting as a fusion product with a molecular mass of 318 kDa. The N-terminal part of the ORF 1a product contains a papain-like cysteine proteinase (PCP) domain with a predicted cleavage site between Gly-619 and Ser-620. The PCP and the upstream protein domains can be aligned with the equivalent parts of the leader proteins encoded by the whitefly-transmitted lettuce infectious yellows and sweet potato sunken vein closteroviruses. Phylogenetic reconstruction based on the aligned RNA polymerase sequences clearly suggests that the aphid-transmissible and whiteflytransmissible closteroviruses represent two distinct evolutionary lineages, with the mealybug-transmissible LChV being the most remote member of the ' whitefly ' lineage.Little cherry disease (LCD) is distributed worldwide in ornamental and sweet cherry and has a great impact on the fruit quality of infected trees. In infected phloem cells,

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Closteroviridae

Martelli¹,

Candresse²

2010

Encyclopedia of Life Sciences

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Closteroviridae is a family of plant viruses with filamentous, helically constructed particles showing distinct cross‐banding and varying in length from 650 nm (species with fragmented genome) to over 2000 nm (species with monopartite genome). The genome is a single‐stranded, positive‐sense RNA ( ribonucleic acid ), with size varying from 13 000 to nearly 19 000 nucleotides, thus being among the largest known for plant viruses. The family comprises three genera, Closterovirus , Ampelovirus and Crinivirus , which differ in genome type (monopartite, bipartite or tripartite) and size, and in epidemiological behaviour. Closteroviruses are transmitted by aphids; ampeloviruses by pseudococcid mealybugs and soft scale insects and criniviruses by whiteflies. These viruses are mostly phloem‐restricted, are all transmitted with a semipersistent modality regardless of the type of vector and are pathogenic to a wide range of herbaceous and woody crops, to which they may cause extensive damage. Control is based on preventive measures such as sanitary selection, sanitation and induction of resistance, primarily through conventional breeding. Key concepts: Closteroviridae is a growing family of plant viruses with three genera. All members of the family have filamentous virions with open particle structures and distinct cross‐banding. Closteroviral genomes are among the largest for plant viruses and are highly diversified also within each genus. The family represents a monophyletic lineage that has evolved in a modular way. A characterizing trait of the genera is the type of vectors that mediate transmission from host to host. Virus particles are primarily restricted to the phloem from where they are acquired by the vectors. Virus dissemination over long distances is mediated by infected propagative material, rather than vectors. Most members of the family have a restricted natural host range. Many cause serious, if not devastating, epidemics to herbaceous and woody crops. Disease control relies on preventive measures aimed at reducing vector populations and sources of inoculum. Sanitary selection and sanitation are efficient means for producing certified stocks.

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Beet Yellows Closterovirus: Complete Genome Structure and Identification of a Leader Papain-like Thiol Protease

Cited by 162 publications

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Nucleotide sequence of the 3'-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus.

Nucleotide sequence of the 3'-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus.

Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus.

Closteroviridae

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