Behavior, Histopathology and Physiological Responses of Rat Fed Diets Containing Growth Hormone Transgenic Fish Meal

Abstract

The animal model response against genetically modified product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into five treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pelleted diet containing 15% (NT-15) and 45% non-transgenic Ccm (NT-45), a re-pelleted diet containing 15% (TG-15) and 45% GH transgenic Ccm (TG-45). Rats were kept for three weeks, fed twice a day according to treatment by 30 g/day/rat and water was provided ad-libitum. Rat behavior was observed every day during feeding. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea, and creatinine were measured at initial and the end of the experiment. Histology observation was made for liver, kidney, and brain tissues. Our results showed that behavior, histopathology, SGOT, SGPT, urea and creatinine levels of rat fed with control, NT- and TG-Ccm diets were similar. All rats survived until the end of the experiment. Thus, within the dose level of this study, GH transgenic Ccm showed no toxicity or effect on behavior and physiology of Wistar rats.

Hepatoprotective Effects of Sukari Dates Extract (Phoenix dactylifera L.) Against ALT (Alanine aminotransferase) and AST (Aspartate aminotransferase) Profile in Male Wistar Rats After Induced by Meloxicam

“…However, it is possible to draw the conclusion that there were differences among all groups based on the average results. The increase in ALT profile occurred in K2 which had been induced by toxic doses of meloxicam, but the increase in ALT profile was still in the normal range of 63-175 U/L in male wistar rats aged 3-4 months [14]. The toxic dose of 30 mg/kg BW of meloxicam causes a rise in the ALT profile in rats, but this rise has not yet reached the point of causing liver damage.…”

“…After being induced by a toxic dose of meloxicam, sukari date extract at a dose of 500 mg/kg BW was able to lower the AST profile in P1. However, dose of 1000 mg/kg BW at P2 may be more effective in lowering the AST profile, making it the optimal dose that can be used as a hepatoprotective to neutralize damage to the liver caused by the administration of a toxic dose of meloxicam with the results of the examination of the AST profile which feel to the normal range of 74-143 U/L in male wistar rats aged 3-4 months [14]. In K2 only given meloxicam, there was a significant increase above the normal range of the AST profile.…”

Pengembangan Pelet Berbasis Nabati sebagai Pakan Alternatif untuk Intervensi Diet Vegetarian pada Tikus Putih (Rattus norvegicus)

“…Bahan makanan diet vegetarian masing-ISSN: 2356-069X E-ISSN: 2715-4343 DOI: 10.33059/jj. v10i1.7411 masing tipe dihaluskan sedangkan pakan standar dihaluskan kembali hingga menjadi tepung (Alimuddin et al, 2019). 75% (15 g/ekor/hari) pakan standar dicampurkan dengan 25% (5 g/ekor/hari) bahan makanan vegetarian sesuai tipe diet menggunakan mixer hingga homogen.…”

“…Terdapat 4 perlakuan diet, yaitu diet vegetarian tipe quasivegetarian, lacto-ovo-vegetarian, vegan, dan omnivora. Perubahan bentuk diet pada masingmasing kelompok dilakukan selama 4 minggu dan diadaptasikan hingga minggu ke-8 (Alimuddin et al, 2019). Penelitian menggunakan sampel 28 ekor tikus putih yang diletakkan pada kandang berlabel sesuai kelompok perlakuan diet berikut.…”

“…Hal ini disebabkan oleh komponen asam-asam amino penyusun protein menjadi prekursor sintesis protein struktural tubuh tikus putih, sehingga semakin tinggi kandungan protein dalam diet vegetarian tertentu, maka semakin tinggi pula proses sintesis protein struktural (Mas'ud & Parakkasi, 2009). Tambahan lagi, apabila konsumsi protein diimbangi dengan olahraga, maka dengan bantuan hormon pertumbuhan serta testosteron, pembentukan protein struktural semakin besar (Alimuddin et al, 2019). Selain itu, diet vegetarian yang rendah lemak juga menunjukkan keuntungan dalam menekan prevalensi peningkatan penyakit kronis seperti kanker, stroke, gagal jantung, dan diabetes serta menurunkan angka kematian (Pawlak, 2017).…”