Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS

Abstract

Background Clinical epidemiological studies have found that some patients with ulcerative colitis (UC) are prone to mental disorders. DSS-induced acute and chronic UC models are often used to evaluate the efficacy of anti-UC drugs. However, whether DSS has an effect on mouse behavior has not been reported. Methods Acute and chronic UC models were induced by 3% DSS and 1.5% DSS, respectively. The bloody stool, the changes in the colon length, and histopathological changes in the colon were used to evaluate the success of the animal model. The behavior of mice was evaluated by open field experiment, tail suspension experiment and Sucrose preference test. Results The weight of mice in 3% DSS group decreased significantly, the DAI score increased significantly, the colon length of mice was significantly shortened, and the structure of colonic crypts was abnormal, which showed inflammatory cell infiltration and shrinkage of crypts. Compared with the control group, the immobility time of 3%DSS group mice in the tail suspension test and forced swimming test had no effect, the number of running and grooming times was significantly reduced, and there was no significant difference in the number of standing times. No abnormality was observed in HE staining of the hippocampus. However, in 1.5% DSS-induced chronic UC model, behavioral and hippocampal abnormalities were observed not only UC symptoms. Conclusions The acute UC model induced by 3% DSS has certain influence on the behavior of mice, but the mental state of mice is normal, which may be the abnormal behavior caused by UC symptoms; However, the chronic UC model induced by 1.5% DSS has a significant effect on the behavior of mice, and the mice have mental disorders, which are caused by mental disorders.

Combined ROS Responsive Polydopamine-Coated Berberine Nanoparticles Effective Against Ulcerative Colitis in Mouse Model

“…The mice exhibited symptoms and pathological characteristics similar to those of chronic UC induced clinically after modeling. 48 After treatment with BBR and PDA@BBR NPs, the symptoms of DSS-induced ulcerative colitis were alleviated, and the pathological tissue examination also showed varying degrees of repair in colonic goblet cells and crypts, with reduced inflammatory cell infiltration. 49 Among these parameters, including changes in mouse body weight, DAI score, and colon length, it was found that PDA@BBR NPs had a more effective therapeutic effect on UC than BBR ( Figure 7 ).…”

Silencing ANGPT2 alleviates ulcerative colitis by regulating autophagy-mediated NLRP3 inflammasome inactivation via the mTOR signaling pathway

“…After one week of adaptation, male C57BL/6 mice (6-8 weeks of age, 20±2 g) were randomly divided into four groups (n=5/group): control group, dextran sodium sulfate (DSS) group, DSS + sh-NC (negative control) group, and DSS + sh-ANGPT2 group. Mice in the DSS group were fed normally and given 3% DSS diluted in water for 7 days ( 8 ). Mice in the DSS + sh-ANGPT2 group received 3% DSS and were injected with adeno-associated virus (AAV) shRNA targeting ANGPT2 (sh-ANGPT2) into the tail vein at the same time.…”

Early-Life Gut Inflammation Drives Sex-Dependent Shifts in the Microbiome-Endocrine-Brain Axis

“…Previously, Salvo et al modeled pediatric IBD by administering 2% DSS for 5 days at weaning (P21) as a model of a single acute period of inflammation (Salvo et al, 2020). Studies in adult rodents have utilised several rounds of DSS to emulate recurring cycles of inflammation and remission (Eichele and Kharbanda, 2017;Okayasu et al, 1990;Zhou et al, 2023). Therefore, we adapted a model in which DSS is administered in the drinking water repeatedly across early postnatal development starting at postnatal day 21 (P21) (Ruemmele and Turner, 2014).…”

“…The widely-used dextran sodium sulfate (DSS)-induced colitis model damages the gut epithelial lining and mimics the gut inflammation and microbiota changes seen in human UC (Chassaing et al, 2014). Adult mice treated with DSS have increased anxiety (Nyuyki et al, 2018) and reduced sucrose preference, indicative of a depression endophenotype (Zhou et al, 2023). Additionally, active gut inflammation in adult mice led to recognition memory impairments in both acute (Emge et al, 2016) and chronic DSSinduced IBD models (Bercik et al, 2011).…”