Behavioral, Biochemical, and Histological Evaluation of Artificial Light on Infected Freshwater Snails Bimophalaria alexandrina by Schistosoma mansoni

al., Ahmed A. A. Hussein et

doi:10.21608/ejabf.2022.252866

Cited by 2 publications

(1 citation statement)

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“…Thermo Nicolet iS10 spectrophotometer examination using Fourier transform infrared spectroscopy (FTIR) was used to detect the composition and functional groups in charge of decreasing or stabilizing the synthesized nanoparticles. The dried nanoparticles were examined using a potassium bromide (KBr) pressed disk with wavelengths ranging from 400 to 4000/cm [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

The Molluscicidal Activity of Green Synthesized Copper Oxide–Based Annona squamosa Seed Extract Nanoparticles on the Feeding Behavior, Biochemical, Molecular, and Immunohistochemical Alterations of Biomphalaria alexandrina Snails

Hussein,

Abd El-latif,

Saad El-Din

et al. 2023

Biol Trace Elem Res

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Because of their low ecological impact, plant molluscicides have garnered much attention. The work aimed to find out if Annona squamosa (AS) seed extract has a molluscicidal impact on Biomphalaria alexandrina snails and enhances this extract by adding CuO nanoparticles (NPs). Using a scanning electron microscope (SEM), transmission electron microscope (TEM), and PANalytical X’Pert PRO X-ray diffractometer (XRD), the presence of the green A. squamosa-based CuO NPs (AS-CuO NPs) was confirmed. After 24 h of exposure, the half-lethal concentration (LC50) of AS-CuO NPs was more toxic to mature B. alexandrina than the aqueous extract of AS seeds (LC50: 119.25 mg/L vs. 169.03 mg/L). The results show that snails exposed to sublethal doses of AS-CuO NPs at LC10 or LC25 (95.4 or 106.7 mg/L, respectively) had much higher glucose levels and alkaline phosphatase activity than those not exposed. Nevertheless, there was no discernible change in the protein content in general or glycogen phosphorylase production. Histological and immunohistochemical analysis showed that snails exposed to A. squamosa-derived CuO NPs LC10 had shrinking digestive tubules and degeneration as well as vacuolation of many digestive, secretory, ova, and sperm cells, with PCNA expressing positively in the hermaphrodite gland and digestive tubule cells. The toxic profile of green CuO NPs produced by A. squamosa may damage the biological activity of B. alexandrina snails; thus, this compound could be used as a molluscicidal base. Furthermore, B. alexandrina proved to be a useful biomarker of nanomaterial contamination.

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Section: Methodsmentioning

confidence: 99%