Behavioral, Molecular, and Morphological Evidence for a Hybrid Zone Between &lt;I&gt;Chrysochus auratus&lt;/I&gt; and &lt;I&gt;C. cobaltinus&lt;/I&gt; (Coleoptera: Chrysomelidae)

Peterson, M. J.; Dobler, Susanne; Holland, Jeff; Tantalo, Lauren C.; Locke, Stefanie E.

doi:10.1603/0013-8746(2001)094[0001:bmamef]2.0.co;2

Cited by 25 publications

(49 citation statements)

References 16 publications

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“…To determine the paternity (conspecific vs. heterospecific) of the offspring of multiply-mated females, we genotyped larvae via cellulose acetate electrophoresis, enabling us to genotype each larva at each of two allozyme loci (malate dehydrogenase (MDH) and aspartate aminotransferase (AAT)), both having species-specific alleles (Peterson et al 2001). Larval tissues were ground in 5 ll distilled water in preparation for loading onto cellulose acetate plates (Titan III, Helena Laboratories, Beaumont, TX).…”

Section: General Proceduresmentioning

confidence: 99%

“…For these estimates, we drew from data in the present study as well as the results of previous work (Peterson et al 2001(Peterson et al , 2005a.…”

Section: Relative Strength Of Reproductive Barriersmentioning

confidence: 99%

“…The sole North American members of this genus, Chrysochus auratus (F.) and C. cobaltinus LeConte are sister species that, based on mtDNA sequence data (Dobler and Farrell 1999) appear to have diverged 2-3 mya (Peterson et al 2001). These otherwise broadly allopatric species form a *75 km-wide hybrid zone in the Yakima River valley of south-central Washington State (Peterson et al 2001(Peterson et al , 2005a. Available evidence suggests that this hybrid zone formed within the past 13,000 years via secondary contact (Peterson et al 2005b).…”

Section: Introductionmentioning

confidence: 99%

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Cryptic gametic interactions confer both conspecific and heterospecific advantages in the Chrysochus (Coleoptera: Chrysomelidae) hybrid zone

et al. 2011

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Most species pairs are isolated through the collective action of a suite of barriers. Recent work has shown that cryptic barriers such as conspecific sperm precedence can be quite strong, suggesting that they evolve quickly. However, because the strength of multiple barriers has been formally quantified in very few systems, the relative speed with which conspecific sperm precedence evolves remains unclear. Here, we measure the strength of both conspecific sperm precedence and cryptic non-competitive isolation between the hybridizing sister species, Chrysochus auratus and C. cobaltinus (Coleoptera: Chrysomelidae), and compare the strength of those barriers to the strength of other known reproductive barriers in this system. Overall, cryptic barriers in this system are weaker than other barriers, indicating that they have not evolved rapidly. Furthermore, their evolution has been asymmetric. Non-competitive barriers substantially reduce the production of hybrid offspring by C. auratus females but not by C. cobaltinus females. In multiply-mated C. cobaltinus females, heterospecific sperm outcompete conspecific sperm, as evidenced by the fact that heterospecific males sired disproportionately more offspring than predicted from the results for singly-mated females. In C. auratus females, neither sperm type has a competitive advantage. Such asymmetries explain why nearly all F1 hybrids in the field are from crosses between C. cobaltinus females and C. auratus males. We discuss these findings in terms of understanding the cost of mating 'mistakes' in the Chrysochus hybrid zone. In addition, our discovery that 95% confidence intervals for commonly-used isolation statistics can be very wide has important implications for speciation research. Specifically, to avoid biases in the interpretation of such isolation metrics, we suggest that studies should routinely include error estimates in their analyses of reproductive isolation.

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Section: General Proceduresmentioning

confidence: 99%

“…For these estimates, we drew from data in the present study as well as the results of previous work (Peterson et al 2001(Peterson et al , 2005a.…”

Section: Relative Strength Of Reproductive Barriersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cryptic gametic interactions confer both conspecific and heterospecific advantages in the Chrysochus (Coleoptera: Chrysomelidae) hybrid zone

et al. 2011

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“…These beetles form a hybrid zone in Washington State (WA) (U.S.A.) (Peterson et al 2001) and are highly promiscuous. Males and females mate daily over their six to eight week lifespan (Dickinson 1995(Dickinson , 1997, during which time males actively search for females, and attempt to copulate upon encounter (Peterson, pers.…”

Section: Introductionmentioning

confidence: 99%

Profiles of cuticular hydrocarbons mediate male mate choice and sexual isolation between hybridising Chrysochus (Coleoptera: Chrysomelidae)

et al. 2007

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Chemical signals frequently underlie sexual isolation between insect species. Our understanding of the evolutionary forces influencing these signaling systems is known for very few systems, challenging both our efforts to understand insect speciation, and our ability to predict longterm changes in the chemical communication systems of insects. Thus, we are in need of more systems in which both the chemical signals causing sexual isolation and the evolutionary forces driving sexual isolation are understood. Sexual isolation in the hybrid zone between Chrysochus cobaltinus and C. auratus has apparently increased in response to natural selection against hybridisation (i.e. reinforcement). Previous experiments suggested that this isolation was due, at least in part, to male preferences for conspecific females. Here, we confirm this role of male choice, and document that male mate choice in this system is influenced by cuticular hydrocarbon (CHC) profiles. Specifically, male C. cobaltinus responses to control cadavers and conspecific female cadavers painted with different cuticular hexane extracts, together with analyses of the composition of those extracts, revealed that male mate choice is governed by CHC profiles. Multivariate analyses of GC profiles demonstrated that those profiles are indeed both sex-and species-specific. Although GC-MS enabled identification and quantification of the specific cuticular hydrocarbons, we have not yet determined which individual compounds govern mate choice. Having established that CHCs influence sexual isolation in this system, we can now assess the evolutionary lability of these cues, which will inform both our understanding of speciation, and of the conditions under which the chemical signaling systems that influence mate choice in insects can evolve.

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“…However, in these examples hybrid formations are still possible between the two host races. Moreover, an interspecific hybridization is known from Diabrotica virgifera and D. longicornis in laboratory (Krysan & Guss 1978), and hybrids between the leaf beetle species Chrysochus auratus and C. cobaltinus were found in nature (Peterson et al 2001). The leaf beetle species Chrysomela lapponica (L.) belongs to the C. interrupta-group, which is distributed mainly in the Nearctic.…”

Section: Introductionmentioning

confidence: 99%

Reproductive isolation between populations from Northern and Central Europe of the leaf beetle Chrysomela lapponica L.

2006

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Behavioral, Molecular, and Morphological Evidence for a Hybrid Zone Between <I>Chrysochus auratus</I> and <I>C. cobaltinus</I> (Coleoptera: Chrysomelidae)

Cited by 25 publications

References 16 publications

Cryptic gametic interactions confer both conspecific and heterospecific advantages in the Chrysochus (Coleoptera: Chrysomelidae) hybrid zone

Cryptic gametic interactions confer both conspecific and heterospecific advantages in the Chrysochus (Coleoptera: Chrysomelidae) hybrid zone

Profiles of cuticular hydrocarbons mediate male mate choice and sexual isolation between hybridising Chrysochus (Coleoptera: Chrysomelidae)

Reproductive isolation between populations from Northern and Central Europe of the leaf beetle Chrysomela lapponica L.

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