Behavioral profile and gastrointestinal evaluation of the hydro-alcoholic extract of Sida rhombifolia L. (typychá hû) in mice

Abstract

The aim of this work was to determine the acute toxicity of hydro-alcoholic extract of S. rhombifolia L. (EHASr), and its influence on general behavior, sleeping time induced by pentobarbital, intestinal transit migration and the effect on spontaneous contractile response of intestinal smooth muscles isolated from mice. Neither oral doses up to 3.000 mg/kg nor intraperitoneal dose up to 1.000 mg/kg denoted toxic symptoms, respectively. Oral administration of 100.0 mg/kg of EHASr promotes statistically significant increase of charcoal marker migration (** p <0.01) in the intestinal transit test. By other hand, individual doses of 0.001, 0.005, 0.01 and 0.05 mg/mL of EHASr did not modify the spontaneous contractile responses of ileum and jejunum muscles. However, the recovery contractile response of both smooth muscles to ACh 10 -7 M, were increased significantly according to each EHASr pre-treatment The presences of alkaloids, tannins, saponins and steroids and/or triterpenes were detected by qualitative phytochemical assay of EHASr. In conclusion, based on results, oral administration of EHASr is safe, well tolerated and increased the intestinal migration of charcoal marker in mice. This finding is correlated to the popular use of S. rhombifolia and encourages us to perform further specific chemical and pharmacological studies.