Behavioral profile assessment in offspring of Swiss mice treated during pregnancy and lactation with caffeine

Abstract

The association between caffeine consumption and various psychiatric manifestations has long been observed. The objective was to assess the behavioral profile in offspring of Swiss mice treated during pregnancy and lactation with caffeine. For this purpose, two groups (n = 6 each and BW ~ 35 g) of female mice were treated during pregnancy and lactation by: tap water and caffeine solution at a concentration of 0.3 mg/mL through oral route. The offspring obtained, by completing 70 days of life, was underwent a behavioral battery test. Statistical analysis was performed by student t test and the different significance adopted was p < 0.05. According to our results, it was not found any significant differences in tail suspension and forced swimming tests. In anxiety related responses however, the mice of caffeine group had greater number of fecal pellets (178 %, p = 0.001) in the open field test, higher number of attempts (51 %, p = 0.03) in light-dark box and decreased percentage of entries in open arms (41 %, p = 0.01) in elevated plus maze test. Moreover, in the marble burying test, there was a significant decrease in the number of buried marbles compared with controls (110 %, p = 0,002). In the meantime, in the von Frey test, it was observed an exacerbation of mechanical allodynia both in basal conditions and after the carrageenan administration (p < 0.001). Furthermore, caffeine treatment during pregnancy and lactation causes long-term behavioral changes in the mice offspring that manifest later in life.

Caffeine Consumption Influences Lidocaine Action via Pain-Related Voltage-Gated Sodium Channels: An In Vivo Animal Study

“…This result is consistent with the variable findings from previous studies that described the effects of caffeine on thermal and mechanical sensations. Caffeine reduced mechanical allodynia in carrageenan-stimulated mice [ 14 ]. Caffeine also exerted short-term antinociceptive effects on the paw withdrawal latency after exposure to a noxious thermal stimulus [ 10 ].…”

The adenosine hypothesis of schizophrenia into its third decade: From neurochemical imbalance to early life etiological risks

“…Exposure to caffeine in drinking water (75, 300, or 1,000 mg/L) in pregnant mice was sufficient to induce working memory deficits and potentiate locomotor response to acute cocaine in the resulting offspring upon reaching adulthood (Björklund et al, 2008 ; Soellner et al, 2009 ). Even perinatal exposure during lactation could modify depressive-like behavior of the offspring upon reaching adulthood, although female offspring appeared more vulnerable, and the effects were bi-directional depending on dosage (Laureano-Melo et al, 2016 ; Magenis et al, 2022 ). Nonetheless, DNA damage in the brain of adult offspring was observed regardless of sex, and thus strongly implicating that caffeine consumption during pregnancy could be detrimental to fetal brain development.…”

Effects of Volatile Organic Compounds (VOCs) of <i>Cinnamomum burmannii</i> in Its Natural State on Physical and Mental Health

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