Benchtop X-ray fluorescence imaging as a tool to study gold nanoparticle penetration in 3D cancer spheroids

Pedrosa, Pedro; Baptista, Pedro V.; Fernandes, Alexandra R.; Guerra, Mauro

doi:10.1039/d1ra05446e

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…XFI allows for the non-invasive detection of contrast agents constituted of specific elements, based on their characteristic X-ray emissions [17]. This spectral specificity constitutes a valuable property for analyzing biodistribution and concentration in preclinical studies [18][19][20][21][22][23][24][25]. Contrary to positron emission tomography (PET) and single photon emission computed tomography (SPECT), the possibility of tracking non-decaying markers permits longitudinal studies of the same subjects over long timespans [22].…”

Section: Introductionmentioning

confidence: 99%

Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

Arsana,

Saladino,

Brodin

et al. 2024

IJMS

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Diffraction-limited resolution and low penetration depth are fundamental constraints in optical microscopy and in vivo imaging. Recently, liquid-jet X-ray technology has enabled the generation of X-rays with high-power intensities in laboratory settings. By allowing the observation of cellular processes in their natural state, liquid-jet soft X-ray microscopy (SXM) can provide morphological information on living cells without staining. Furthermore, X-ray fluorescence imaging (XFI) permits the tracking of contrast agents in vivo with high elemental specificity, going beyond attenuation contrast. In this study, we established a methodology to investigate nanoparticle (NP) interactions in vitro and in vivo, solely based on X-ray imaging. We employed soft (0.5 keV) and hard (24 keV) X-rays for cellular studies and preclinical evaluations, respectively. Our results demonstrated the possibility of localizing NPs in the intracellular environment via SXM and evaluating their biodistribution with in vivo multiplexed XFI. We envisage that laboratory liquid-jet X-ray technology will significantly contribute to advancing our understanding of biological systems in the field of nanomedical research.

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Section: Introductionmentioning

confidence: 99%

Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

Arsana,

Saladino,

Brodin

et al. 2024

IJMS

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“…Only such these resolutions can solve many biological problems and explain the mechanisms biologists encounter in cell biology [1,2]. Many wellestablished imaging techniques in the major areas of optical microscopy, electron-based microscopy, and scanning-probe microscopy fulfill this requirement [3][4][5][6][7]. One of them is soft X-ray microscopy, which provides a unique set of capabilities between visible light and electron microscopy [8].…”

Section: Introductionmentioning

confidence: 99%

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

et al. 2022

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Soft X-ray microscopy is a powerful technique for imaging cells with nanometer resolution in their native state without chemical fixation, staining, or sectioning. The studies performed in several laboratories have demonstrated the potential of applying this technique for imaging the internal structures of intact cells. However, it is currently used mainly on synchrotrons with restricted access. Moreover, the operation of these instruments and the associated sample-preparation protocols require interdisciplinary and highly specialized personnel, limiting their wide application in practice. This is why soft X-ray microscopy is not commonly used in biological laboratories as an imaging tool. Thus, a laboratory-based and user-friendly soft X-ray contact microscope would facilitate the work of biologists. A compact, desk-top laboratory setup for soft X-ray contact microscopy (SXCM) based on a laser-plasma soft X-ray source, which can be used in any biological laboratory, together with several applications for biological imaging, are described. Moreover, the perspectives of the correlation of SXCM with other super-resolution imaging techniques based on the current literature are discussed.

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Multifunctional gold nanoparticles for cancer theranostics

Fernandes

2024

3 Biotech

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Benchtop X-ray fluorescence imaging as a tool to study gold nanoparticle penetration in 3D cancer spheroids

Abstract: X-Ray fluorescence imaging and quantification was used to assess diffusion patterns in differently coated Au nanoparticles in 3D cancer spheroids.

Cited by 5 publications

References 35 publications

Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

Multifunctional gold nanoparticles for cancer theranostics

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