Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation

Abstract

The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p<0.05). Results showed that ECA-coated groups presented better results, reducing C. albicans biofilm formation. Acquired images revealed that these groups (ECA1 and ECA2) presented a reduced number of cells, mostly in yeast form (less pathogenic), while the other groups presented higher number of cells, mostly in hyphae form (more pathogenic). Based on these findings, a beneficial effect of Super Bonder® coating reline resins surface could be demonstrated, suggesting a promising way to prevent fungal biofilm formation on dentures.

Effect of surface sealant on surface roughness and bacterial adhesion of bulk-fill composites

“…Among tested restorative materials, lower bacterial adhesion was observed with the application of BLV, which is an anti-adherent, low-viscosity, and ethanol-based sealant. Even though the effect of BLV against Candida albicans over dentures has been proven in many studies, 10,40 researches against the S. mutans biofilm on composite materials involving BLV are very rare. Furthermore, it appears that this is the first study to investigate the effect of BLV on bacterial adhesion of bulk-fill composites.…”

Longitudinal Evaluation of Ethyl Cyanoacrylate Adhesives on Candida albicans Biofilm

“…The cyanoacrylate adhesives evaluated in this study have been successfully used in surgical procedures, both in medicine and dentistry (Thakeb et al, 1995;Pascual et al, 2016) for the control of hemorrhages (Al-Bawardy et al, 2016). Recently, cyanoacrylates and fibrin biopolymers have also been studied for use as denture coating adhesives (Ali et al;Soon et al, 2015;Távora et al).…”

“…Authors have reported the coating of PMMA surfaces (Ali et al, 2013;Távora et al, 2019) In this context, the possibility of applying cyanoacrylate-based adhesives as coatings on acrylic resins was considered, thus modifying its surface properties, reducing roughness, and increasing hydrophobicity (Távora et al). However, no study has assessed its long-term efficacy.…”

Thermal stability and non‐isothermal kinetics of poly(ethyl cyanoacrylate) nanofibers

“…A new wound dressing system based on poly(lactic acid)‐modified cyanoacrylate that is easily removable has been proposed 8 . A useful effect of ethyl cyanoacrylate (ECA) coating reline resin surfaces has been described, suggesting a promising way to prevent fungal biofilm formation on dentures 9 . Recently, the efficacy of a polycyanoacrylate porous material for bone tissue substitution was demonstrated 10 .…”

“…8 A useful effect of ethyl cyanoacrylate (ECA) coating reline resin surfaces has been described, suggesting a promising way to prevent fungal biofilm formation on dentures. 9 Recently, the efficacy of a polycyanoacrylate porous material for bone tissue substitution was demonstrated. 10 The monomers occur as clear and colorless liquids with low viscosity, high volatility and strong reactivity.…”