Beneficial Effects of Time and Calorie Restriction Diets on the Development of Experimental Acute Kidney Injury in Rat: Bax/Bcl-2 and Histopathological Evaluation

Abstract

People's lifestyles and, especially, their eating habits affect their health and the functioning of the organs in their bodies, including the kidneys. One's diet influences the cells' responses to stressful conditions such as acute kidney injury (AKI). This study aims to determine the preconditioning effects of four different diets: calorie restriction (CR) diet, time restriction (TR) eating, intermittent fasting (IF), and high-fat diet (HF) on histopathological indices of the kidney as well as the molecules involved in apoptosis during AKI. Adult male rats underwent CR, TR, IF, and HF diets for eight weeks. Then, AKI was induced, and renal function indices, histopathological indices, and molecules involved in apoptosis were measured. After AKI, urinary albumin excretion, serum urea, creatinine and, Bax/Bcl-2 ratio increased in the kidney, while renal GFR decreased. CR and TR diets improved renal parameters and prevented an increase in the Bax/Bcl-2 ratio. The IF diet improved renal parameters but had no effect on the Bax/Bcl-2 ratio. On the other hand, the HF diet worsened renal function and increased the Bax/Bcl-2 ratio. Histopathological examination also showed improved kidney conditions in the CR and TR groups and more damage in the HF group. This study demonstrated that CR and TR diets have renoprotective effects on AKI and possibly cause the resistance of kidney cells to damage by reducing the Bax/Bcl-2 ratio and improving apoptotic conditions.

A late eating midpoint is associated with increased risk of diabetic kidney disease: a cross-sectional study based on NHANES 2013–2020

“…Alireza and his colleagues conducted two trials of dietary interventions in treating experimental acute kidney injury (AKI), and found that restricting feeding for 5 h had renoprotective effects to rats with AKI by inhibiting the apoptosis of kidney cells, improving antioxidant status, and inhibiting renal fibrosis [43,44]. Likewise, receiving short-term early time-restricted feeding could also exert the reno-protective effects of anti-oxidation and inhibition of renal fibrosis to rats by regulating mitochondrial dynamics [45].…”

The anticancer effects of thymol on HepG2 cell line

“…As is well known, these two counteracting members of the bcl-2 family are key factors in apoptotic cell death [31]. The researchers have shown that it is not only the levels of individual proteins but also their ratio (bax/bcl-2) which determines susceptibility to apoptosis [32]. bax is a pro-apoptotic protein that responds to apoptotic signals by activating the intrinsic pathway of apoptosis.…”