Benefits from Tween during enzymic hydrolysis of corn stover

Kaar, William E.; Holtzapple, Mark T.

doi:10.1002/(sici)1097-0290(19980820)59:4<419::aid-bit4>3.0.co;2-j

Cited by 232 publications

(117 citation statements)

References 11 publications

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…This means a fact that cellulase in solution is increased by surfactant addition and the cellulose conversion is also increased simultaneously. On the other hand, Karr and Holtzapple (1998) reported that Tween 20 works as 'lignocellulose disrupter' that can increase the availability of reaction site. Helle et al (1993) also reported that surfactant can disrupt H-bonding of cellulose which results in more cellulose accessible to the enzymes.…”

Section: The Role Of Tween 20mentioning

confidence: 99%

See 1 more Smart Citation

Effects of a non-ionic surfactant, Tween 20, on adsorption/desorption of saccharification enzymes onto/from lignocelluloses and saccharification rate

2011

View full text Add to dashboard Cite

In this work, we examined the role of a non-ionic surfactant, Tween 20, on enzymatic hydrolysis of lignocelluloses. Delignified lignocelluloses (pine wood chip) were used as model substrates. Effects of Tween 20 on adsorption/desorption onto/from lignocelluloses with and without hydrolysis were evaluated respectively. Tween 20 lowered the non-biospecific adsorption of β-glucosidase and enhanced the bio-specific adsorption of cellulase. Tween 20 did not affect the liquid phase reaction (cellobiose hydrolysis). However, for the solid surface reaction (cellulose hydrolysis), cellulose conversion for 72 hrs was increased 9-21% and 1-8.5% for samples with high lignin contents (PI) and low lignin contents (PIII) by injection of Tween 20 (0.024-0.24 mM), respectively. Moreover, Tween 20 increased the cellulose conversion rate substantially. It is suggested that the increase of cellulase amount adsorbed due to the increase of effective cellulose surface by Tween 20 contribute to the enhancement of cellulose conversion.

show abstract

Section: The Role Of Tween 20mentioning

confidence: 99%

“…surfactants, PEGs; polyethylene glycols and BSA; bovine serum albumin, etc.) (Börjesson et al 2007a(Börjesson et al , 2007bEriksson et al 2002;Helle et al 1993;Karr and Holtzapple 1998;Kumar and Wyman 2008;Mizutani et al 2002;Park et al 1992;Yang and Wyman 2006;Zheng et al 2008aZheng et al , 2008b.…”

mentioning

confidence: 99%

Effects of a non-ionic surfactant, Tween 20, on adsorption/desorption of saccharification enzymes onto/from lignocelluloses and saccharification rate

2011

View full text Add to dashboard Cite

show abstract

“…An efficient pretreatment of lignocellulosic residues includes to reduction of lignin content, decrease of crystallinity, and increase surface area for enzymatic reactions [4,5]. Chemical and mechanical methods (e.g., acid [3,6], steam [7], alkali [3], and ammonium freeze explosion (AFEX) [8]) for the pretreatment of sugar cane and agave bagasses have been described. Pretreatment of the lignocellulosic substrates should consider endoglucanase, cellobiohydrolase, b-glucosidase and different hemicellulolytic activities which play important roles in the efficiency of hydrolysis [9].…”

Section: Introductionmentioning

confidence: 99%

Efficient chemical and enzymatic saccharification of the lignocellulosic residue from Agave tequilana bagasse to produce ethanol by Pichia caribbica

Saucedo-Luna

Castro‐Montoya

Martínez-Pacheco

et al. 2010

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Bagasse of Agave tequilana (BAT) is the residual lignocellulosic waste that remains from tequila production. In this study we characterized the chemical composition of BAT, which was further saccharified and fermented to produce ethanol. BAT was constituted by cellulose (42%), hemicellulose (20%), lignin (15%), and other (23%). Saccharification of BAT was carried out at 147 °C with 2% sulfuric acid for 15 min, yielding 25.8 g/l of fermentable sugars, corresponding to 36.1% of saccharificable material (cellulose and hemicellulose contents, w/w). The remaining lignocellulosic material was further hydrolyzed by commercial enzymes, ~8.2% of BAT load was incubated for 72 h at 40 °C rendering 41 g/l of fermentable sugars corresponding to 73.6% of the saccharificable material (w/w). Mathematic surface response analysis of the acid and enzymatic BAT hydrolysis was used for process optimization. The results showed a satisfactory correlation (R (2) = 0.90) between the obtained and predicted responses. The native yeast Pichia caribbica UM-5 was used to ferment sugar liquors from both acid and enzymatic hydrolysis to ethanol yielding 50 and 87%, respectively. The final optimized process generated 8.99 g ethanol/50 g of BAT, corresponding to an overall 56.75% of theoretical ethanol (w/w). Thus, BAT may be employed as a lignocellulosic raw material for bioethanol production and can contribute to BAT residue elimination from environment.

show abstract

“…Results of studies on the effect of surfactants were very diverse, and nonionic surfactants were more preferable for free of any electrostatic interaction [15]. Moreover, some nonionic surfactants were reported to increase the catalytic activity or selectivity of enzymes [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Improving Enzymatic Production of Ginsenoside Rh2 from Rg3 by using Nonionic Surfactant

Wang

2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

In this study, several nonionic surfactants were tried to improve the enzymatic hydrolysis of ginsenoside Rg3 into Rh2 which was catalyzed at 50 degrees C and pH 5.0 by a crude glucosidase extracted from Fusarium sp. ECU2042. Among the biocompatible nonionic surfactants, polyethylene glycol 350 monomethyl ether was shown to be the best. After optimizing some influencing factors on the reaction, the conversion of Rg3 (5 g/l) with 10 g/l crude enzyme reached almost 100% in the presence of the nonionic surfactant (7.5%, w/v), which was 25% higher than that in buffer without any surfactant. Furthermore, the enzyme stability was affected faintly by the surfactant.

show abstract

Benefits from Tween during enzymic hydrolysis of corn stover

Cited by 232 publications

References 11 publications

Effects of a non-ionic surfactant, Tween 20, on adsorption/desorption of saccharification enzymes onto/from lignocelluloses and saccharification rate

Effects of a non-ionic surfactant, Tween 20, on adsorption/desorption of saccharification enzymes onto/from lignocelluloses and saccharification rate

Efficient chemical and enzymatic saccharification of the lignocellulosic residue from Agave tequilana bagasse to produce ethanol by Pichia caribbica

Improving Enzymatic Production of Ginsenoside Rh2 from Rg3 by using Nonionic Surfactant

Contact Info

Product

Resources

About