Berberis lycium fruit extract attenuates oxi-inflammatory stress and promotes mucosal healing by mitigating NF-κB/c-Jun/MAPKs signalling and augmenting splenic Treg proliferation in a murine model of dextran sulphate sodium-induced ulcerative colitis

Sharma, Anamika; Tirpude, Narendra Vijay; Kulurkar, Pankaj Markand; Sharma, Rohit; Padwad, Yogendra

doi:10.1007/s00394-019-02114-1

Cited by 31 publications

(22 citation statements)

References 37 publications

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“…Data showed that PPS evidently ameliorated the colitis as manifested by the lowered DAI and prolonged colon, and relieved the intestinal adhesion, hyperemia, edema, and ulceration of the colitis-suffering mice. Moreover, the colon histological structure was destroyed with declined of PCNA-positive cells in the DSS-exposed mice, which was in agreement with previous reports (Jin et al, 2017;Maria-Ferreira et al, 2018;Sharma, Tirpude, Kulurkar, Sharma, & Padwad, 2019). (Fakhoury, Negrulj, Mooranian, & Al-Salami, 2014).…”

Section: Discussionsupporting

confidence: 92%

Polysaccharide from Pycnoporus sanguineus ameliorates dextran sulfate sodium‐induced colitis via helper T cells repertoire modulation and autophagy suppression

Luo

Huang

et al. 2020

Phytotherapy Research

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Inflammatory bowel disease (IBD) is a chronic autoimmune disease associated with various risk factors. Pycnoporus sanguineus (L.) Murrill is a saprotrophic fungus used worldwide for its industrial and medical purposes. Here, polysaccharide from P. sanguineus (PPS) was explored for its antiinflammatory potential in a murine colitis model of IBD induced by dextran sulfate sodium (DSS). PPS ameliorated the colitis as manifested by the lowered disease activity index (DAI), prolonged colon, and reduced serum lipopolysaccharide (LPS). PPS recovered the histological lesion by upregulating the expressions of Zonula occludens-1 (ZO-1), E-cadherin, and proliferating cell nuclear antigen (PCNA). PPS inhibited the helper T cells (Th)-mediated immune response by decreasing the proportions of Th cells (including Th2 cells, Th17 cells, and regulatory T cells), which was accompanied with reductions on myeloperoxidase (MPO) activity and releases of several interleukins and chemokines within the colon. Moreover, PPS exhibited an evident inhibition on autophagy, in which the ratio of light chain 3 (LC3) II/I was declined, while the expression of p62 and Beclin-1 was increased. The present study highlighted important clinical implications for the treatment application of PPS against IBD, which relies on the regulation of Th cells repertoire and autophagy suppression to restore epithelium barrier.

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Section: Discussionsupporting

confidence: 92%

Polysaccharide from Pycnoporus sanguineus ameliorates dextran sulfate sodium‐induced colitis via helper T cells repertoire modulation and autophagy suppression

Luo

Huang

et al. 2020

Phytotherapy Research

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“…In addition, the myeloperoxidase activity, the expression level of IL-1β, IL-12p40 and IFN-γ, as well as the infiltration of CD3+T cells and macrophages in the colonic mucosa were decreased in MCP-1 deficient mice. Meanwhile, a decrease of MCP-1 expression was often reported in studies about the treatment of UC [19,20]. Our study found a significant decrease in the expression of MCP-1 in the colon tissue of UC rats supplemented with Cit.…”

Section: Plos Onesupporting

confidence: 74%

Protective effects of citrulline supplementation in ulcerative colitis rats

Cai

Zhou²,

Huang

et al. 2020

PLoS ONE

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It has been reported that supplementing certain amino acids has therapeutic effects on ulcerative colitis (UC). We intend to explore whether citrulline (Cit) supplementation has protective effects on UC. Fifteen male Wistar rats were divided into normal control group (NC group), UC group and UC+Cit group, with five rats in each group. The UC model was established by TNBS/ethanol method. Rats in UC+Cit group were intragastrically administered with Cit for 7 consecutive days after modeling. All rats were sacrificed after 7 days. Blood samples were collected to detect the number of monocytes. Colon tissues were taken for HE staining. Immunohistochemistry staining for CD68 and p-STAT3 were performed to detect the infiltration of monocytes and the phosphorylation of STAT3 in colon tissues. The concentrations of MCP-1, IL-6 and IL-17A and the protein expression of p-STAT3 in colon tissues were measured by ELISA and western blot methods, respectively. The body weight of UC group rats decreased significantly after 7 days (p<0.05). However, the weight loss of UC+Cit group rats was not statistically significant (p>0.05). The number of peripheral blood monocytes in UC+Cit group was significantly lower than that in UC group (p<0.05), and the infiltration of CD68-positive monocytes in the colon tissue of UC+Cit group was significantly reduced than that in UC group. The concentrations of MCP-1, IL-6 and IL-17A and the expression of p-STAT3 in colon tissues of UC+Cit group rats were significantly lower than those in UC group (both p<0.05). Our study suggests that Cit supplementation may be a potential therapy for UC.

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“…Furthermore, HE staining demonstrated that the colon of mice in the MG group was severely damaged compared to those in the NG group, while no significant structural disorders were observed in the RG and PG groups ( Figure 2E ). PCNA was further applied to assess the proliferation level of intestinal epithelial cells ( Sharma et al, 2020 ), and PCNA of the MG group was found to be lower than that of the NG group, while the PCNA in the colon of the RG group and the PG group was higher ( Figure 2F ).…”

Section: Resultsmentioning

confidence: 99%

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway

Zhu

Zheng

et al. 2021

Front. Pharmacol.

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Ulcerative colitis (UC) is a chronic inflammatory disease that affects the colon, and its incidence is on the rise worldwide. Resveratrol (RSV), a polyphenolic compound, was recently indicated to exert anti-inflammatory effects on UC. Consequently, the current study was conducted to investigate the mechanism of RSV on alleviating UC in mice by mediating intestinal microflora homeostasis. First, potential targets that RSV may regulate UC were screened using the TCMSP database. Next, mice were treated differently, specifically subjected to sham-operation and dextran sulfate sodium (DSS) induction, and then treated or untreated with RSV. Disease Activity Index (DAI) and Hematoxylin-Eosin (HE) staining were employed to analyze the pathological changes of mice colon. In addition, the expression patterns of inflammatory factors in spleen tissues were detected using ELISA, while the protein expression patterns of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and vascular endothelial growth factor A (VEGFA) in colon tissues were determined by means of immunohistochemistry (IHC) and Western blot analysis. Moreover, changes in intestinal flora and metabolite diversity in UC were analyzed by metabonomics. It was found that RSV played inhibitory roles in the PI3K/Akt pathway in mice. Meanwhile, the administration of RSV induced downregulated the expressions of TNF-α, IFN-γ, IL-1β, IL-6, and IL-4. The six floras of Haemophilus and Veillonella were significantly enriched in UC, while Clostridium, Roseburia, Akkermansia, and Parabacteroides were found to be enriched in control samples. Lastly, it was noted that Akkermansia could regulate the intestinal flora structure of UC mice through triacylglycerol biosynthesis, glycerol phosphate shuttle, cardiolipin biosynthesis, and other metabolic pathways to improve UC in mice. Altogether, our findings indicate that RSV suppressed the activation of the PI3K/Akt pathway and reduced the VEGFA gene expression to alleviate UC in mice.

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Berberis lycium fruit extract attenuates oxi-inflammatory stress and promotes mucosal healing by mitigating NF-κB/c-Jun/MAPKs signalling and augmenting splenic Treg proliferation in a murine model of dextran sulphate sodium-induced ulcerative colitis

Cited by 31 publications

References 37 publications

Polysaccharide from Pycnoporus sanguineus ameliorates dextran sulfate sodium‐induced colitis via helper T cells repertoire modulation and autophagy suppression

Polysaccharide from Pycnoporus sanguineus ameliorates dextran sulfate sodium‐induced colitis via helper T cells repertoire modulation and autophagy suppression

Protective effects of citrulline supplementation in ulcerative colitis rats

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway

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