Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures

Pujia, Arturo; Russo, Cristina; Maurotti, Samantha; Pujia, Roberta; Mollace, Vincenzo; Romeo, Stefano; Montalcini, Tiziana

doi:10.3390/nu10091305

Cited by 14 publications

(4 citation statements)

References 49 publications

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“…Furthermore, when miR-27b-3p was overexpressed, the expression of CTNNB1 decreased, which confirmed our previous hypothesis. In agreement with these findings, the relationship between CTNNB1 and endometriosis has been reported in several studies [ 29 , 30 , 31 , 32 ], which is related to the Wnt and the ERK1/2 pathways [ 33 , 34 ]. To the best of our knowledge, CTNNB1 [ 11 ] plays an important role in the adhesion, invasion, and metastasis of endometrial tissues via the Wnt/β-catenin signaling pathway and could even improve the border of extraovarian endometriosis lesions [ 35 ].…”

Section: Discussionsupporting

confidence: 81%

Long Intergenic Non-Protein Coding RNA 02381 Promotes the Proliferation and Invasion of Ovarian Endometrial Stromal Cells through the miR-27b-3p/CTNNB1 Axis

Wang

Zeng

et al. 2022

Genes

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Purpose: Catenin Beta 1 (CTNNB1) is a key regulator of cell proliferation and invasion in endometriosis; however, its upstream factor is not clear. Long noncoding RNAs may participate in endometriosis. The aim of this study was to investigate the mechanism of interaction between LINC02381 and CTNNB1 in endometriosis. Method: Screening and validation of RNAs were completed by whole transcriptional sequencing and qRT-PCR. The subcellular localization of LINC02381 was determined by RNA in situ hybridization and nucleo-cytoplasmic separation. Plasmids were transfected for functional experiments. Luciferase assay was used to verify the binding relationship. Results: The expression of LINC02381 and CTNNB1 was significantly increased in ovarian ectopic endometrial tissues (OSAs) and ectopic endometrial stromal cells (ESCs). When LINC02381 was downregulated in ESCs, the expression of CTNNB1, metallopeptidase 9 (MMP9) and cyclinD1, as well as ESCs invasion and proliferation, decreased. LINC02381 was mainly present in the cytoplasm of ESCs, indicating that it may act as a competitive endogenous RNA. Bioinformatic analysis revealed that microRNA-27b-3p (miR-27b-3p) is a downstream target of LINC02381. miR-27b-3p decreased in OSAs and ESCs. Moreover, when miR-27b-3p was upregulated in ESCs, the expression of CTNNB1, MMP9 and cyclinD1, as well as the invasion and proliferation ability of ESCs, were reduced. Additionally, rescue experiments demonstrated that the expression of CTNNB1, MMP9 and cyclinD1, as well as the invasion and proliferation ability, were significantly increased in the group transfected with both sh-LINC02381 and a miR-27b-3p inhibitor. Conclusion: LINC02381 upregulated CTNNB1 by adsorbing miR-27b-3p, causing increased proliferation and invasion of ESCs.

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Section: Discussionsupporting

confidence: 81%

Long Intergenic Non-Protein Coding RNA 02381 Promotes the Proliferation and Invasion of Ovarian Endometrial Stromal Cells through the miR-27b-3p/CTNNB1 Axis

Wang

Zeng

et al. 2022

Genes

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“…BPF also reduces RANKL expression, suggesting that it may inhibit osteoclastogenesis. Further studies are necessary to validate this study [35].…”

Section: Further Beneficial Impacts Of the Bergamot-derived Polypheno...mentioning

confidence: 79%

“…Neutralization of the bone-specific WNT inhibitor is currently being evaluated as a promising anabolic therapy for patients with osteoporosis [34]. A study by Pujia et al (2018) examined the effects of bergamot polyphenols in bone cell models and showed that RUNX2, β-catenin, ERK 1/2, and intracellular COL1A proteins are up-regulated by exposure to increasing doses of BPF. In addition, the expression of RANKL, which is a known stimulus for increased osteoclast activity, is reduced in these cells, suggesting that BPF decreases RANKL and may play a potential role in bone resorption.…”

Section: Further Beneficial Impacts Of the Bergamot-derived Polypheno...mentioning

confidence: 99%

Bergamot Polyphenols: Promising Agents for Health and Wellness

Gruca,

Wais,

Zając

et al. 2024

J Educ Health Sport

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Introduction and purpose: Citrus bergamia, commonly known as bergamot, is a unique plant native to the Calabria region in Italy, prized for its fragrant fruits and versatile applications. Historically utilized for extracting essential oil, bergamot has garnered attention for its therapeutic properties, particularly its Bergamot-derived Polyphenolic Fraction (BPF), which exhibits lipid-lowering, antioxidant, and anti-inflammatory effects. This paper aims to provide a comprehensive summary of existing research on the therapeutic potential of BPF in humans.Brief description of the state of knowledge: BPF has shown promise in supporting wound healing, alleviating neuropathic pain, and exhibiting antimicrobial properties. Research highlights BPF's role in modulating lipid metabolism, particularly in reducing LDL cholesterol and triglycerides while increasing HDL cholesterol levels. Additionally, BPF demonstrates anti-inflammatory effects, accelerating the recovery process in conditions such as non-alcoholic steatohepatitis (NASH) and potentially influencing pathways involved in cancer cell proliferation and apoptosis. Further studies explore BPF's impact on bone metabolism, erectile dysfunction, and its potential in dermatological applications, particularly in counteracting photoaging.Conclusions: While existing studies underscore the promising therapeutic effects of BPF in various medical domains, further research is warranted to establish its efficacy and safety for broader clinical use. Continued investigation on a larger scale holds the potential to develop BPF as a valuable physiotherapeutic strategy across diverse medical fields.

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“…To date, the protective effects of the main polyphenols contained in high concentration in bergamot are well-known. In particular, important beneficial effects on the nervous system [ 41 , 42 , 43 ], the mineralization of bones [ 44 ], the regulation of keratinocytes [ 45 ], the regulation of the metabolism [ 46 , 47 ], against adipose-tissue inflammation [ 48 ] and during myocardial regeneration [ 49 ] were reported. These protective effects of bergamot have been found in many of its formulations: extract (BE), juice (BJ), essential oil (BEO), and polyphenolic fraction (BPF).…”

Section: Introductionmentioning

confidence: 99%

Effects of Bergamot Polyphenols on Mitochondrial Dysfunction and Sarcoplasmic Reticulum Stress in Diabetic Cardiomyopathy

et al. 2021

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Cardiovascular disease is the leading cause of death and disability in the Western world. In order to safeguard the structure and the functionality of the myocardium, it is extremely important to adequately support the cardiomyocytes. Two cellular organelles of cardiomyocytes are essential for cell survival and to ensure proper functioning of the myocardium: mitochondria and the sarcoplasmic reticulum. Mitochondria are responsible for the energy metabolism of the myocardium, and regulate the processes that can lead to cell death. The sarcoplasmic reticulum preserves the physiological concentration of the calcium ion, and triggers processes to protect the structural and functional integrity of the proteins. The alterations of these organelles can damage myocardial functioning. A proper nutritional balance regarding the intake of macronutrients and micronutrients leads to a significant improvement in the symptoms and consequences of heart disease. In particular, the Mediterranean diet, characterized by a high consumption of plant-based foods, small quantities of red meat, and high quantities of olive oil, reduces and improves the pathological condition of patients with heart failure. In addition, nutritional support and nutraceutical supplementation in patients who develop heart failure can contribute to the protection of the failing myocardium. Since polyphenols have numerous beneficial properties, including anti-inflammatory and antioxidant properties, this review gathers what is known about the beneficial effects of polyphenol-rich bergamot fruit on the cardiovascular system. In particular, the role of bergamot polyphenols in mitochondrial and sarcoplasmic dysfunctions in diabetic cardiomyopathy is reported.

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Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures

Cited by 14 publications

References 49 publications

Long Intergenic Non-Protein Coding RNA 02381 Promotes the Proliferation and Invasion of Ovarian Endometrial Stromal Cells through the miR-27b-3p/CTNNB1 Axis

Long Intergenic Non-Protein Coding RNA 02381 Promotes the Proliferation and Invasion of Ovarian Endometrial Stromal Cells through the miR-27b-3p/CTNNB1 Axis

Bergamot Polyphenols: Promising Agents for Health and Wellness

Effects of Bergamot Polyphenols on Mitochondrial Dysfunction and Sarcoplasmic Reticulum Stress in Diabetic Cardiomyopathy

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