Bermuda Grass as a Potential Reservoir Host forGrapevine fanleaf virus

Abstract

Grapevine fanleaf virus (GFLV) was detected in samples of Bermuda grass (BG) from Iran by reverse transcription-polymerase chain reaction (RT-PCR) using two different pairs of GFLV-specific primers, and also by enzyme-linked immunosorbent assay (ELISA) using antiserum specific for a North American isolate of the virus. RT-PCR detected GFLV in both fresh and dried BG tissues and in virus preparations purified from these plants. Cloning and sequencing of the RT-PCR products confirmed that the amplified sequences were sections of the GFLV coat protein gene. Similar results were obtained when random and oligo(dT) primers were used on viral RNA templates recovered from BG to synthesize cDNA for cloning and sequencing. The virus induced few or no symptoms in BG, but could nonetheless be transmitted from BG to Chenopodium quinoa by mechanical inoculation. Some isolates induced systemic chlorotic spots and leaf deformation; others remained symptomless in this plant. Both symptomatic and symptomless C. quinoa plants were found to be infected with GFLV, giving positive ELISA and RT-PCR tests. A North American isolate of GFLV was found to be mechanically transmissible to BG as indicated by positive RT-PCR results from root samples of inoculated plants. GFLV-infected BG was widely distributed in the Fars province of Iran.

Xiphinema index and its Relationship to Grapevines: A review

“…The virus was detected by RT-PCR using two different pairs of GFLV specific primers and ELISA. However, the Bermuda grass expressed few or no symptoms of GFLV infection (Izadpanah et al, 2003). In addition to X. index, X. italiae has been reported to spread GFLV , but these results were not corroborated (Esmenjaud & Bouquet, 2009).…”

Distinct phylogenetic positions of Grapevine fanleaf virus isolates from Iran based on the movement protein gene

“…With respect to GFLV isolates from Iran, only the CP gene has so far been partially sequenced (Izadpanah et al 2003). The goal of the current study was to survey vineyards in the northwest region of Iran for GFLV and to investigate genetic variation in the MP of representative isolates.…”

Molecular Characterization of Phylogenetically Distinct Isolates of Grapevine fanleaf virus from Iran Based on 2AHP Gene

“…To that end, various methods have been developed for detecting GFLV which include ELISA [7], reverse transcription polymerase chain reaction (RT-PCR) [28], RNA oligo probe capture RT-PCR [8] and IC-RT-PCR [41]. In Iran, grapes are produced at the rate of 2.1 million tons annually on about 270,000 ha [17]. The North West region of Iran shares a major part of the produce and therein grape industry has been established.…”

“…The use of 5 0 NC and GMPR1 was expected to produce a fragment of 1,995 bp encompassing 181 nts of 5 0 UTR plus HP and MP genes. The primer cp912 [17] corresponds to nucleotides of 2,936-2,959 of GFLV-F13 RNA2.…”