Bestimmung der Plasmaelimination von Hydroxyaethylstärke und von Dextran mittels verbesserter analytischer Methodik

Förster, H.; Wicarkzyk, C.; Dudziak, R.

doi:10.1159/000221190

Cited by 17 publications

(20 citation statements)

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“…The average Mw in vivo is the same and determined by MS only. Although differences in frequency of side effects could be possible, a statement should not be allowed in general [1,8,[25][26][27]. The results of this study are further arguments to evaluate HES solutions only by regarding the MS and not by regarding the average Mw.…”

Section: Discussionmentioning

confidence: 93%

“…Intracellular lysis after tissue storage especially in sessile or circulating cells of the reticuloendothelial system is another possibility [20]. Tissue storage of HES was also described in organs with effective immunological and detoxicating abilities like liver, spleen, or lung [8,21]. Lysosomal degradation after intracellular storage is possible.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, a persisting fraction is characteristic for HES and can not be found for dextran. Pharmacokinetics of dextran can be described with a kinetic function of first order [8].…”

Section: Discussionmentioning

confidence: 99%

“…A sample of about 40 ml was taken from the urine of each collection period and stored frozen. HES concentration in serum samples was analysed enzymatically after acid hydrolysis and differential precipitation with ethanol [8]. Molecular masses were determined by Size-Exclusion-Chromatography (SEC) (a combination of high performance liquid chromatography (HPLC) and gel permeation chromatography (GPC)) with a consecutive multi angle light laser scattering (MALLS) and refraction index (RI) detector [9].…”

Section: Methodsmentioning

confidence: 99%

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Pharmacokinetics of Hydroxyethyl Starch (70/0.5) following Repeated Infusions

Lehmann¹,

Asskali²,

Förster³

2003

Transfus Med Hemother

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Background: For the purpose of haemodilution different medium substituted hydroxyethyl starches (HES) are available. These plasma substitutes can be further characterized by their average molecular weight (Mw) into medium Mw (HES 200/0.5) and low Mw (HES 70/0.5) starches. Patients and Methods: Pharmacokinetic data were collected in a trial with a design similar to clinical use. 8 healthy volunteers were given 50 g HES on 5 consecutive days (=250 g) to estimate the in vivo changes of the chemical structures of HES (70/0.5). Results: Calculated half-life of serum elimination were 6–10 h which was representative for medium substituted HES and the chosen infusion scheme. The mild accumulation of HES (70/0.5) should be without clinical relevance. The in vivo Mw is equivalent to the in vitro Mw of the infused HES (70/0.5). The in vivo Mw is about 60,000 Dalton for most medium substituted HES. Nevertheless, regarding molar substitution and pattern of substitution (C2:C6) there are some differences between applied HES (70/0.5) and HES recovered in urine. These changes also indicate a shift in the chemical properties of infused HES in the organism. Only 60% of infused HES were recovered in urine. The destination of the remaining 40% (∼100 g in this clinical trial) is still unexplained. A fractional amount might be stored in the reticuloendothelial system. Concerning the measured data of medium substituted HES (70/0.5) it stands to reason that differences in the approximate substituted HES (200/0.5) with a higher molecular weight are without clinical relevance. Conclusion: The in vivo Mw is independent of the in vitro Mw of the applied HES. Pharmacokinetic data suggest that HES (70/0.5) is a good option for the purpose of haemodilution.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

“…Consequently, a persisting fraction is characteristic for HES and can not be found for dextran. Pharmacokinetics of dextran can be described with a kinetic function of first order [8].…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Pharmacokinetics of Hydroxyethyl Starch (70/0.5) following Repeated Infusions

Lehmann¹,

Asskali²,

Förster³

2003

Transfus Med Hemother

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“…The hexokinase/glucose-6-phosphat-dehydrogenase method was used to determine HES serum concentration in the control mares following the splitting of the polysaccharide using HCl acid (Foerster et al, 1981). The pharmacokinetic evaluation was carried out with the help of the Topfit 2.0 research program (Heinzel et al, 1993).…”

Section: Data Collectedmentioning

confidence: 99%

Effect of Hydroxyethyl Starch Solution in Normal Horses and Horses with Colic or Acute Colitis

Schusser

Rieckhoff

Ungemach

et al. 2007

Journal of Veterinary Medicine Series A

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SummaryHydroxyethyl starch (HES) solution is an effective colloidal infusion solution in humans for treatment of hypovolaemic shock, but it has not been compared with fluids currently available for use in horses. On the basis of plasma-expanding effect of HES in normal horses, a 10% medium-molecular 200/ 0.5 solution of HES was subsequently tested in hypovolaemic horses. Six normal horses were given five protocols of a single infusion of HES at varying dosage rates (5, 10, 15 ml HES/kg), as well as isotonic saline (15 ml/kg) and hypertonic saline (4 ml/kg b.w.). Dehydrated horses suffering from acute colitis or those which had been treated surgically for ileus of the small or large intestine were given an i.v. infusion of 10 ml HES/kg in combination with 10 ml saline/kg. Clinical data and blood samples for testing were taken before the infusion, and then 10 min, 1 h, 2, 4, 6, 8, 10, 12 and 24 h after infusion (a.i.). A significant decrease in haematocrit was observed in protocol 1-5 for a period of up to 4, 4, 10 h, 10 min and up to 10 min; in group of colitis, during the entire 24-h testing period, and in groups of ileus of small intestine and of large intestine, up to 4 and 10 h a.i. HES decreases better and longer-lasting haematocrit and total protein than either isotonic or hypertonic saline. Half-life of HES increases due to higher dosage (5.83, 7.63 and 11.48 h) and distribution is exclusively intravascular. In normal horses of protocol 1-3 using HES aPTT, sodium and potassium were within the physiological range. Serum amylase activity is increased in horses using HES. On the basis of this clinical study, the decreasing effect of urea and creatinine in colic patients after surgery and fewer instances of postoperative ileus a dosage of 10 ml HES/kg could be recommended.

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Rapid HPLC method for acetyl starch determination in blood

2001

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SummaryColloidal plasma substitutes of chemically modified starch are used in surgery and in emergency medicine. Acelyl starch (ACS) is a new plasma substitute based on an amylopectin acetic ester. Metabolic cleavage of the ACS ester substituents leads to improved degradation and elimination of infused polymer. To determine the metabolic fate ofACS a rapid LC-method forACS quantitiation in blood samples was needed. For this purpose a size-exclusion chromatography (SEC) system with improved sensitivily is outlined using a refractive index detector. The limit of detection is 0.005 mg mL 1. From 0.10-5.00 mg mL 1 a linear relationship (correlation coefficient R = 0,9999) bel',,veen the RI signal and ACS concentration is obtained. Recoveries of ACS from blood plasma range 102.3-107.7% for ACS 200/0.5 (range 0.20-7.94 mg mL 1) and 103.0-111.4% forACS 200/0.7 (range 0.19-9.33 mg mL 1). Only small differences bel',,veen runs are obtained. In the inter assay test coefficients of variation of 1.8% and of 2.6% respectively are obtained forACS 200/0.5 and ACS 200/0.7.

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Bestimmung der Plasmaelimination von Hydroxyaethylstärke und von Dextran mittels verbesserter analytischer Methodik

Cited by 17 publications

References 0 publications

Pharmacokinetics of Hydroxyethyl Starch (70/0.5) following Repeated Infusions

Pharmacokinetics of Hydroxyethyl Starch (70/0.5) following Repeated Infusions

Effect of Hydroxyethyl Starch Solution in Normal Horses and Horses with Colic or Acute Colitis

Rapid HPLC method for acetyl starch determination in blood

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