Beta Cell Regenerating Potential of Azadirachta indica (Neem) Extract in Diabetic Rats

McCalla, G; Parshad, O; Brown, Petra; Gardner, Michael L. G.

doi:10.7727/wimj.2014.224

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…Interestingly enough, glucose levels did not seem to be restored. This phenomenon seems to go in contrast with what authors mention as previously described antidiabetic effects on -cells [63]. Yet, other researchers have confirmed restorative effects on -cells when using Neem extract [64].…”

Section: Anti-cancerous Effectcontrasting

confidence: 80%

“…Streptozotocin (STZ) is a potent compound known for its preferential toxicity to -cells due to the overwhelming induction of methylation and ROS production, in addition to a gradual decrease in GLUT2 expression [62]. Due to these effects, STZ is a common chemical-inducer of diabetes type 1 in small animals [58,63]. Thus far, using Neem extracts on this model have shown some very…”

Section: Anti-cancerous Effectmentioning

confidence: 99%

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Current Evidence about Neem Extracts, the Molecules and Physiological Mechanisms Their Nutritional Components Interact with

Islas¹,

Acosta²,

Escalante-Acosta³

et al. 2019

Preprint

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Plant-based alternative medicine is normally grounded on empiric cultural perception, as the main objective, these practices intent to either maintain good health or to provide a route to turn-the-tide on a specific disease or ailment. Amongst the thousands of plants that have been used and studied, Neem (Azadirachta indica) seems to have a very interesting tale to tell, since its properties to ward-off certain diseases have overtime, and in a rigorous way, been proven. The preceding concise review is a collection of some of the most relevant studies today, not only focusing on the health benefits obtained by its use, but digging into the molecular mechanisms of how the properties come about. In particular, we take a look over antioxidant properties and how these mediate and mitigate important molecules such as IL-6 and TNF-, leading the way in reducing systematic damage by oxidative stress. Further, we relate this oxidative reduction to other systemic diseases such as cancer and diabetes, as these are currently becoming the most rampant killers. As of yet not all is known about the different ways of extracting or the total composition of an extract, as these may be from different parts of the plant. Therefore, we also allude to an important cautionary view where toxicological effects and conflicting outcomes arise. Overall, presented results show a great potential for the different extracts of Neem as their antioxidant activity can be taken advantage off, and potentially used in modern medicine.

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Section: Anti-cancerous Effectcontrasting

confidence: 80%

Section: Anti-cancerous Effectmentioning

confidence: 99%

Current Evidence about Neem Extracts, the Molecules and Physiological Mechanisms Their Nutritional Components Interact with

Islas¹,

Acosta²,

Escalante-Acosta³

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…The specimen was assigned voucher number 35688 and deposited in the herbarium. As previously described by McCalla et al, 8 fresh, healthy neem leaves with their stems were collected from The UWI Mona Campus and Botanical Garden (coordinates: 18.0059° N,…”

Section: Plant Collection and Identificationmentioning

confidence: 99%

“…Plant extraction was previously reported by McCalla et al 8 Briefly, the powder from milled, dried leaves and their associated stems was weighed and boiled in distilled water (100 g/L portions) under reflux for four hours. A strainer and muslin cloth were used to filter the cooled mixture.…”

Section: Plant Extractionmentioning

confidence: 99%

“…It seems likely that this is a dose-dependent matter, given that most researchers report antidiabetic properties; however, a low concentration of 0.8% reportedly did not alleviate diabetes. [5][6][7][8] This current study was, therefore, designed to assess the suitability of the T2DM models and the validity of the use of a low-dose extract of neem leaves and associated stems (NLE) as an antidiabetic agent and to determine whether it can alleviate hyperglycemia associated with T2DM.…”

Section: Introductionmentioning

confidence: 99%

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Suitability of the Neonatal Streptozotocin Diabetes Model and Folklore Therapeutic use of Low-Dose Neem Leaf Extract to Treat Hyperglycemia Associated with Type 2 Diabetes Mellitus

2024

TJNPR

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Neem leaf has been used in folklore medicine to combat diabetes mellitus and other ailments. Difficulty finding suitable type 2 diabetes mellitus (T2DM) models led to the exploration of the neonatal streptozotocin (nSTZ) model to investigate the impact of low-dose neem leaf extract (NLE) on T2DM-associated hyperglycemia. Following ethical approval, two-and three-day-old neonatal rat pups (n=66) received 60 mg/kg STZ intraperitoneally while normal controls (n=9) received an equivalent volume of citrate buffer. Weaned animals received free access to chow and water and a constant 12 hour on/12 hour off light cycle. Tail vein blood glucose was assessed weekly following an eight-hour fast, using Accu Chek Advantage glucometer. Animals received tap water (Normal and Diabetic Control) or 0.8 % NLE (Diabetic Treated) for six weeks. The results were expressed as means ± standard error of the mean using the Origin Pro 7.0 software. The significance of the mean difference between groups was determined with the IBM SPSS for Windows software version 23 via the paired samples T-test. Statistical significance of p<0.05 was used. The mortality was 74% pre-STZ (32.6% post-STZ) with 40.9% or 81.8% success (of STZinjected and STZ-survivors), respectively. Fasting blood glucose was significantly (p<0.05) elevated in the diabetic groups and was not alleviated by low-dose NLE. Because of the variability in mortality and success rates, further investigation is warranted for better reproducibility of the nSTZ T2DM model with minimal mortality. Furthermore, low-dose therapeutic NLE does not positively impact hyperglycemia and should not replace clinically prescribed medicines.

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A Comprehensive Pharmacological Appraisal of Indian Traditional Medicinal Plants with Anti-diabetic Potential

Acharya

Das²,

Madhu³

et al. 2023

Advances in Diabetes Research and Management

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Beta Cell Regenerating Potential of Azadirachta indica (Neem) Extract in Diabetic Rats

Abstract: The results suggest that NLE has beta cell regenerating potential.

Cited by 7 publications

References 10 publications

Current Evidence about Neem Extracts, the Molecules and Physiological Mechanisms Their Nutritional Components Interact with

Current Evidence about Neem Extracts, the Molecules and Physiological Mechanisms Their Nutritional Components Interact with

Suitability of the Neonatal Streptozotocin Diabetes Model and Folklore Therapeutic use of Low-Dose Neem Leaf Extract to Treat Hyperglycemia Associated with Type 2 Diabetes Mellitus

A Comprehensive Pharmacological Appraisal of Indian Traditional Medicinal Plants with Anti-diabetic Potential

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