Beta-sitosterol with antimicrobial property from the stem bark of pomegranate (&lt;i&gt;Punica granatum&lt;/i&gt; Linn)

Nweze, C.a.; Ibrahim, H.; Ndukwe, G. I.

doi:10.4314/jasem.v23i6.7

Cited by 10 publications

(8 citation statements)

References 5 publications

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“…Column chromatography and crystallization of fraction 1 (Fr. 1) yielded white needle-like crystals (19 mg) which were separated from the mother liquor and were named compound 1 and identified as β-sitosterol by comparing with previous literature reports . Fraction pool Fr.…”

Section: Resultsmentioning

confidence: 83%

Exploring the Phytochemical Profile and Biological Activities of Clerodendrum infortunatum

et al. 2023

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Clerodendrum infortunatum ( C. infortunatum ), the hill glory bower, is reputed as the prodigious treasure for Indian folk medicine. The study has focused on exploring the phytochemistry and antitumor potential of the C. infortunatum root extract in vitro and in vivo . The ethyl acetate root extract has demonstrated the highest cytotoxicity in a series of nine human tumor cell lines. Further fractionation of the same has yielded seven compounds. The structures of these compounds were confirmed with spectroscopic techniques. Considering the toxicity observed with the crude extract, cytotoxicity of these compounds was further assessed in two breast carcinoma cell lines (MCF-7[ER/PR-positive HER2-negative] and MDA-MB-231 [ER/PR/HER2-negative]) and in two cervical cancer [human papilloma virus (HPV)-negative C33A and HPV-positive SiHa] cell lines. Betulinic acid (BA) was found as the active principle contributing the cytotoxic activity, and cervical cancer cell lines documented the minimum IC 50 value in 24 h. In order to validate the in vitro experimental data, we have established a xenograft model of HPV-positive cervical cancer in female NOD/SCID mice treated with BA using doxorubicin as the positive control. BA treatment gradually reduced the tumor size, maintaining healthy hematological and biochemical parameters, and improved the survival rate of tumor-bearing mice considerably. Thus, our findings suggest that the C. infortunatum root extract has a promising anticancer property against HPV-positive cervical cancer and supports its usage by traditional healers for treating cervical cancer.

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Section: Resultsmentioning

confidence: 83%

Exploring the Phytochemical Profile and Biological Activities of Clerodendrum infortunatum

et al. 2023

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“…It is worth noting that β -sitosterol has antibacterial potential. When studying against Salmonella typhi and Escherichia coli strains, the values of growth inhibition zones were 20 and 35 mm, respectively [ 28 ]. The activity of a β -sitosterol chloroform extract of M. parviflora roots against S. aureus and E. coli was studied [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Chemical Composition and Antimicrobial Activity of Subcritical CO2 Extract of Lepidium latifolium L. (Brassicaceae)

Azimkhanova

Устенова

Sharipov

et al. 2021

International Journal of Biomaterials

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The genus Lepidium L. from Brassicaceae Burnett. family covers over 150 species with an almost cosmopolitan spread. In Kazakhstan, 21 species are described, of which four species are characterized by medicinal properties (L. crassifolium Waldst. et Kit., L. perfoliatum L., L. ruderale L., and L. latifolium L.), used in folk medicine as means of antibacterial, irritant, laxative, antitumor, analgesic, and anthelmintic action. Methods. Raw materials were collected from Almaty region (Republic of Kazakhstan). Lepidium latifolium L. herb’s carbon dioxide extract (CO2 extract) was obtained by subcritical carbon dioxide extraction. A gas chromatograph with a mass spectrometric detector was used to determine the component composition of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilution and the disc-diffusion method. Four microbial test strains were used: Staphylococcus aureus ATCC 6538-P, Escherichia coli ATCC 8739, Klebsiella pneumonia ATCC 10031, and Candida albicans ATCC 10231. Results. The technology of carbon dioxide extraction has undoubted advantages over traditional methods of extraction: it has a controlled selectivity in relation to groups of biologically active substances, allows deep extraction, and maximizes the release of rich complexes of compounds contained in plants. In this study, firstly, the CO2 extract was obtained under subcritical conditions from the aerial part of L. latifolium L., and the composition was determined. Hexane was the best solvent for CO2 extract, and 40 components were identified. Screening of antimicrobial activity of the L. latifolium’s CO2 extract showed the essential activity of all clinically significant strains tested: Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Candida albicans. Conclusions. This research showed that the CO2 extract of the raw material of Lepidium latifolium L. contains biologically active compounds exhibiting an essential antimicrobial effect, and therefore it is possible to recommend for the development of various drugs for use in medical practice.

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“…In addition, there are γ-sitosterol (25.76%), n-hexadecanoic acid (4.27%) and (Z)-11octadecenoic acid (3.16%) are reported to as potent broad spectrum antimicrobial [37][38][39][40][41]. Moreover, it has been demonstrated that the SP extracts have antimicrobial effect and inhibited both Gram-positive and Gram-negative bacteria because the presence of carvacrol ( 0.57%) which is confirmed by Memar, M.Y., et al, 2017 [42].…”

Section: Discussionmentioning

confidence: 99%

The Effectiveness of Salavadora Persica (Siwak) Petroleum Ether Extract As An Intracanal Medicament Used in Endodontic Therapy: An In Vitro Study

Ayoub¹,

Badr²,

Alghamdi³

et al. 2021

Preprint

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Background: Salvadora persica L. (Siwak) of the family Salvadoraceae has been used for many centuries as oral hygiene tools, particularly in Saudi Arabia. The current study aimed at developing a new root canal medicament based on the extract of Salvadora persica (SP). The antibacterial activity against Enterococcus faecalis was determined compared with the gold standard intracanal medicament, Ca (OH)2. Materials and Methods: Freeze-dried Siwak sticks were extracted with Petroleum ether to yield an oily extract (SPE). GC-MS analysis was carried out to identify SPE components, whereas 32 compounds were identified (89.09%), with main components of benzyl isothiocyanate (BITC) (33.32%) and steroids (34%). Firstly, the consistency of the experimental medicament was accomplished according to ANSI/ADA Specification no 57. SPE assessment as intracanal medicament was conducted through an in vitro assay on 45 single-rooted mandibular premolars inoculated of 5 μL of E. faecalis. Colony-forming units (CFU) were counted before the application of experimental intracanal medicament (CFU-1) and then after seven days of its application (CFU-2). Group I: Siwak extract-based intracanal medicament, Group II positive control group received Ca(OH)2 paste, and Group III: negative control group received no medication. Results: CFU before and after using Siwak based medicament recorded statistically significant reduction (P=0.006) as well as those of Group II when Ca(OH)2 was applied (P=0.01). On comparing both medicaments with Group III, there were significant differences; (P=0.00) and (P=0.007), respectively. Siwak oil extract-based medication proved complete eradication of E. faecalis.Conclusion: SPE has a good potential as an intracanal medicament, besides the cleaning and shaping during endodontic therapy. This finding could be referred to as the high content of BITC bactericidal against pulpal and periodontal pathogens, in synergism with other antimicrobial components, representing 70.71% of SP oil extract.

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Beta-sitosterol with antimicrobial property from the stem bark of pomegranate (<i>Punica granatum</i> Linn)

Cited by 10 publications

References 5 publications

Exploring the Phytochemical Profile and Biological Activities of Clerodendrum infortunatum

Exploring the Phytochemical Profile and Biological Activities of Clerodendrum infortunatum

Chemical Composition and Antimicrobial Activity of Subcritical CO2 Extract of Lepidium latifolium L. (Brassicaceae)

The Effectiveness of Salavadora Persica (Siwak) Petroleum Ether Extract As An Intracanal Medicament Used in Endodontic Therapy: An In Vitro Study

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