Beware imposters: MA‐1, a novel MALT lymphoma cell line, is misidentified and corresponds to Pfeiffer, a diffuse large B‐cell lymphoma cell line—A reply: Despite the same 8‐code STR, MA‐1 and Pfeiffer are cytogenetically diverse

Kuo, Sung‐Hsin; Tsai, Hui‐Jen; Weng, Wen‐Hui; Li, Chi‐Cheng; Yeh, Kun‐Huei; Chen, Li-Tzong; Cheng, Ann‐Lii

doi:10.1002/gcc.22122

Cited by 5 publications

(3 citation statements)

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“…To further assess whether t(1;14)(p22;q32)/BCL10‐IGH and t(14;18)(q32;q21)/IGH‐MALT1 are also implicated in the lymphomagenesis of HP‐independent gastric MALT lymphoma, we evaluated the aforementioned genetic translocations in tumour cells of 30 selected cases of gastric MALT lymphoma, including 23 HP‐independent and seven HP‐dependent cases, by interphase fluorescent in situ hybridization (supplementary material, Supplementary materials and methods) . Among 23 HP‐independent cases, we found one case with (1;14)(p22;q32)/BCL10‐IGH and one case with t(14;18)(q32;q21)/IGH‐MALT1.…”

Section: Discussionmentioning

confidence: 99%

“…OCI-Ly3 is a non-germinal centre B-cell origin DLBCL cell line without t(11;18)(q21;q21) [19,20]. MA-1 is a DLBCL cell line that is positive for t(14;18)(q32;q21)/IGH-MALT1, but negative for the nuclear expression of BCL10 and NF-κB (supplementary material, Supplementary materials and methods) [20,21,37,38]. Considering that MALT lymphoma cells are of non-germinal centre B-cell origin [7,24,39] and that t(14;18)(q32;q21)/IGH-MALT1 is a special chromosomal translocation that exists in MALT lymphoma [22,40,41], we selected these two B-cell lymphoma cell lines, OCI-Ly3 and MA-1, to mimic the in vitro environment of t(11;18)(q21;q21)-negative B-cell lymphomas.…”

Section: Cell Lines and Chemicalsmentioning

confidence: 99%

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The B-cell-activating factor signalling pathway is associated withHelicobacter pyloriindependence in gastric mucosa-associated lymphoid tissue lymphoma without t(11;18)(q21;q21)

et al. 2016

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We previously reported that activation of the B-cell-activating factor (BAFF) pathway upregulates nuclear factor-κB (NF-κB) and induces BCL3 and BCL10 nuclear translocation in Helicobacter pylori (HP)-independent gastric diffuse large B-cell lymphoma (DLBCL) tumours with evidence of mucosa-associated lymphoid tissue (MALT). However, the significance of BAFF expression in HP independence of gastric low-grade MALT lymphomas without t(11;18)(q21;q21) remains unexplored. Sixty-four patients who underwent successful HP eradication for localized HP-positive gastric MALT lymphomas without t(11;18)(q21;q21) were studied. BAFF expression was significantly higher in the HP-independent group than in the HP-dependent group [22/26 (84.6%) versus 8/38 (21.1%); p < 0.001]. Similarly, BAFF receptor (BAFF-R) expression (p = 0.004) and nuclear BCL3 (p = 0.004), BCL10 (p < 0.001), NF-κB (p65) (p = 0.001) and NF-κB (p52) (p = 0.005) expression were closely correlated with the HP independence of these tumours. Moreover, BAFF overexpression was significantly associated with BAFF-R expression and nuclear BCL3, BCL10, NF-κB (p65) and NF-κB (p52) expression. These findings were further validated in an independent cohort, including 40 HP-dependent cases and 18 HP-independent cases of gastric MALT lymphoma without t(11;18)(q21;q21). The biological significance of BAFF signalling in t(11;18)(q21;q21)-negative lymphoma cells was further studied in two types of lymphoma B cell: OCI-Ly3 [non-germinal centre B-cell origin DLBCL without t(11;18)(q21;q21) cell line] and MA-1 [t(14;18)(q32;q21)/IGH-MALT1-positive DLBCL cell line]. In both cell lines, we found that BAFF activated the canonical NF-κB and AKT pathways, and induced the formation of BCL10-BCL3 complexes, which translocated to the nucleus. BCL10 and BCL3 nuclear translocation and NF-κB (p65) transactivation were inhibited by either LY294002 or by silencing BCL3 or BCL10 with small interfering RNA. BAFF also activated non-canonical NF-κB pathways (p52) through tumour necrosis factor receptor-associated factor 3 degradation, NF-κB-inducing kinase accumulation, inhibitor of κB kinase (IKK) α/β phosphorylation and NF-κB p100 processing in both cell lines. Our data indicate that the autocrine BAFF signal transduction pathway contributes to HP independence in gastric MALT lymphomas without the t(11;18)(q21;q21) translocation. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Section: Cell Lines and Chemicalsmentioning

confidence: 99%

The B-cell-activating factor signalling pathway is associated withHelicobacter pyloriindependence in gastric mucosa-associated lymphoid tissue lymphoma without t(11;18)(q21;q21)

et al. 2016

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“…For example, NIH grant applicants may be required to provide information on how they intend to address concerns about the identity or contamination of their cell lines. A variety of organizations advocate best practices for handling biological resources (12), including cell lines and cell banking (13,14), yet reports of misidentified or contaminated cell lines still appear in the literature (15,16).…”

Section: Discussionmentioning

confidence: 99%

Cell line authentication to improve preclinical cancer research: Policies and training

Freedman¹,

Siegel²

2015

AACR Education book

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Evaluation genotypes of cancer cell lines HCC1954 and SiHa by short tandem repeat (STR) analysis and DNA sequencing

Cheng

Liu

et al. 2018

Mol Biol Rep

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Beware imposters: MA‐1, a novel MALT lymphoma cell line, is misidentified and corresponds to Pfeiffer, a diffuse large B‐cell lymphoma cell line—A reply: Despite the same 8‐code STR, MA‐1 and Pfeiffer are cytogenetically diverse

Cited by 5 publications

References 7 publications

The B-cell-activating factor signalling pathway is associated withHelicobacter pyloriindependence in gastric mucosa-associated lymphoid tissue lymphoma without t(11;18)(q21;q21)

The B-cell-activating factor signalling pathway is associated withHelicobacter pyloriindependence in gastric mucosa-associated lymphoid tissue lymphoma without t(11;18)(q21;q21)

Cell line authentication to improve preclinical cancer research: Policies and training

Evaluation genotypes of cancer cell lines HCC1954 and SiHa by short tandem repeat (STR) analysis and DNA sequencing

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