Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine

Bao, Ying; Zhang, Dejing; Guo, Huihui; Ma, Wenxue

doi:10.1111/cas.16097

Cited by 13 publications

(1 citation statement)

References 80 publications

(189 reference statements)

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“…Liquid biopsy has been proven in recent years as an effective tool for non-invasive diagnosis and followup for several pathologies in the wide context of personalized medicine [1][2][3]. Sources of samples for liquid biopsy are almost all body fluids, such as plasma, urine, and saliva, which contain several components considered to be biomarkers of the physiopathological state of an individual [4,5].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Surface Functionalizations for Ring Resonator-Based Biosensors

Ardoino,

Lunelli,

Pucker

et al. 2024

Sensors

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Liquid biopsy is expected to become widespread in the coming years thanks to point of care devices, which can include label-free biosensors. The surface functionalization of biosensors is a crucial aspect that influences their overall performance, resulting in the accurate, sensitive, and specific detection of target molecules. Here, the surface of a microring resonator (MRR)-based biosensor was functionalized for the detection of protein biomarkers. Among the several existing functionalization methods, a strategy based on aptamers and mercaptosilanes was selected as the most highly performing approach. All steps of the functionalization protocol were carefully characterized and optimized to obtain a suitable protocol to be transferred to the final biosensor. The functionalization protocol comprised a preliminary plasma treatment aimed at cleaning and activating the surface for the subsequent silanization step. Different plasma treatments as well as different silanes were tested in order to covalently bind aptamers specific to different biomarker targets, i.e., C-reactive protein, SARS-CoV-2 spike protein, and thrombin. Argon plasma and 1% v/v mercaptosilane were found as the most suitable for obtaining a homogeneous layer apt to aptamer conjugation. The aptamer concentration and time for immobilization were optimized, resulting in 1 µM and 3 h, respectively. A final passivation step based on mercaptohexanol was also implemented. The functionalization protocol was then evaluated for the detection of thrombin with a photonic biosensor based on microring resonators. The preliminary results identified the successful recognition of the correct target as well as some limitations of the developed protocol in real measurement conditions.

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Section: Introductionmentioning

confidence: 99%

Optimization of Surface Functionalizations for Ring Resonator-Based Biosensors

Ardoino,

Lunelli,

Pucker

et al. 2024

Sensors

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Liquid biopsy—A biomarker‐based revolutionising technique in cancer therapy

Sarkar,

Chakraborty,

Ghosh

et al. 2024

Clinical and Translational Dis

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Background and AimsCancer has grabbed the attention of scientists and medical professionals all over the world much more than any other disease. In the past few decades, the medical field has improved quite a lot but progress in the path to find a solution for cancer is very less. As the popularity of invasive technologies is diminishing in cancer treatment, scientists have come up withminimally invasive or non‐invasive alternatives, among which liquid biopsy, by far is the most suitable.MethodsLiquid biopsy is used to analyse nucleic acids, subcellular components and circulating tumour cells in various biological fluids for diagnosis of cancer. It can also be used to know the efficacy of cancer drugs in a patient by analysing multiple samples.OutcomesLiquid biopsy is becoming standard of care as it allows biopsy of those samples in which solid tumour biopsies are not possible. The diversity of sampling procedures, such as collection of urine for urothelial carcinoma or bladder or prostate cancer and phlebotomy for other types of cancer, make liquid biopsy one of the best methods for diagnosis of cancer.ConclusionThis review aims in discussing the several techniques used for the detection of cancer biomarkers and some clinical manifestations due to the changes in the biomarkers which are analysed by liquid biopsy.

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