Beyond luciferase-luciferin system: Modification, improved imaging and biomedical application

Jiang, Yiyi; Shi, Xiaorui; Tang, Chu; Wang, Fu

doi:10.1016/j.ccr.2023.215045

Cited by 12 publications

(12 citation statements)

References 185 publications

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“…Supported by the photographs of collected tumors and their corresponding tumor weight comparison (Figures D and E), HCP was proven to have fairly good antitumor efficacy. Given that the inoculated cells were luciferase-decorated 4T1 (LUC-4T1) cells, we also simultaneously evaluated the efficacy of HCP by in vivo imaging using d -luciferin firefly (LUC). , It was shown that the luminescent intensity declined obviously after injecting HCP and the intratumor injection seemed to have a better therapeutic effect than the intravenous injection (Figures S20–S22). To further investigate the therapeutic mechanism of HCP, tumor slices randomly chosen from different groups were stained to assess its antitumor effect, respectively.…”

Section: Resultsmentioning

confidence: 99%

Microfluidic Synthesis of CuH Nanoparticles for Antitumor Therapy through Hydrogen-Enhanced Apoptosis and Cuproptosis

He,

Pan,

Zeng

et al. 2024

ACS Nano

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Cuproptosis has drawn enormous attention in antitumor material fields; however, the responsive activation of cuproptosis against tumors using nanomaterials with high atom utilization is still challenging. Herein, a copper-based nanoplatform consisting of acid-degradable copper hydride (CuH) nanoparticles was developed via a microfluidic synthesis. After coating with tumor-targeting hyaluronic acid (HA), the nanoplatform denoted as HA-CuH-PVP (HCP) shows conspicuous damage toward tumor cells by generating Cu + and hydrogen (H 2 ) simultaneously. Cu + can induce apoptosis by relying on Fenton-like reactions and lead to cuproptosis by causing mitochondrial protein aggregation. Besides, the existence of H 2 can enhance both cell death types by causing mitochondrial dysfunction and intracellular redox homeostatic disorders. In vivo experimental results further exhibit the desirable potential of HCP for killing tumor cells and inhibiting lung metastases, which will broaden the horizons of designing copper-based materials triggering apoptosis and cuproptosis for better antitumor efficacy.

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Section: Resultsmentioning

confidence: 99%

Microfluidic Synthesis of CuH Nanoparticles for Antitumor Therapy through Hydrogen-Enhanced Apoptosis and Cuproptosis

He,

Pan,

Zeng

et al. 2024

ACS Nano

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“…Finally, it must be mentioned that many BL transformations have been utilized as detection systems in bioanalytical applications and bioimaging [ 116 , 117 , 118 , 119 ]. These BL systems include the well-studied firefly BL, where an intramolecular CIEEL-like chemiexcitation mechanism appears to operate, and the coelenterazine BL, where different mechanisms are postulated [ 1 , 51 , 120 , 121 , 122 ].…”

Section: General Chemiexcitation Mechanismsmentioning

confidence: 99%

The Molecular Basis of Organic Chemiluminescence

et al. 2023

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Bioluminescence (BL) and chemiluminescence (CL) are interesting and intriguing phenomena that involve the emission of visible light as a consequence of chemical reactions. The mechanistic basis of BL and CL has been investigated in detail since the 1960s, when the synthesis of several models of cyclic peroxides enabled mechanistic studies on the CL transformations, which led to the formulation of general chemiexcitation mechanisms operating in BL and CL. This review describes these general chemiexcitation mechanisms—the unimolecular decomposition of cyclic peroxides and peroxide decomposition catalyzed by electron/charge transfer from an external (intermolecular) or an internal (intramolecular) electron donor—and discusses recent insights from experimental and theoretical investigation. Additionally, some recent representative examples of chemiluminescence assays are given.

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“…In order to address these limitations, the technique of bioluminescence resonance energy transfer (BRET) has been devised to amplify luminous intensity and adjust the maximum emission wavelength. [6] BRET is a photophysical phenomenon that generates additional light emission through nonradiative energy transfer between the excited bioluminophore donor in luciferase and a chromophore acceptor in the fluorescent protein. Consequently, this approach offers a potential solution to enhance the capabilities of bioluminescence imaging.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Insights into the Bacterial Luciferase‐based Bioluminescence Resonance Energy Transfer Luminescence: The Role of Protein Complex Dimer

Luo,

Pi,

Liu

2024

ChemPhysChem

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Bacterial bioluminescence holds significant potential in the realm of optical imaging due to the inherent advantages of bioluminescence and ease of operation. However, its practical utility is hindered by its low light intensity. The fusion of bacterial luciferase with a highly fluorescent protein has been demonstrated to significantly enhance autonomous luminescence. Nevertheless, the underlying mechanism behind this enhancement remains unclear, and there is a dearth of research investigating the mechanistic aspects of bioluminescence resonance energy transfer (BRET) luminescence, whether it occurs naturally or can be achieved through experimental means. In this study, we investigated the phenomenon of bacterial luciferase‐based BRET luminescence employing a range of computational techniques, including structural modeling, molecular docking, molecular dynamics simulations, as well as combined quantum mechanics and molecular mechanics calculations. The theoretical findings suggest that the BRET luminescence occurs through resonance energy transfer between the excited bioluminophore and the ground chromophore within the protein complex dimer. The proposed mechanism of the protein complex dimer offers a microscopic understanding of the intriguing BRET phenomenon and has the potential to inspire further practical applications in the field of optical imaging.

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Beyond luciferase-luciferin system: Modification, improved imaging and biomedical application

Cited by 12 publications

References 185 publications

Microfluidic Synthesis of CuH Nanoparticles for Antitumor Therapy through Hydrogen-Enhanced Apoptosis and Cuproptosis

Microfluidic Synthesis of CuH Nanoparticles for Antitumor Therapy through Hydrogen-Enhanced Apoptosis and Cuproptosis

The Molecular Basis of Organic Chemiluminescence

Mechanistic Insights into the Bacterial Luciferase‐based Bioluminescence Resonance Energy Transfer Luminescence: The Role of Protein Complex Dimer

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