Beyond Sensitive and Selective Electrochemical Biosensors: Towards Continuous, Real-Time, Antibiofouling and Calibration-Free Devices

Self Cite

The multifaceted key roles of cytokines in immunity and inflammatory processes have led to a high clinical interest for the determination of these biomolecules to be used as a tool in the diagnosis, prognosis, monitoring and treatment of several diseases of great current relevance (autoimmune, neurodegenerative, cardiac, viral and cancer diseases, hypercholesterolemia and diabetes). Therefore, the rapid and accurate determination of cytokine biomarkers in body fluids, cells and tissues has attracted considerable attention. However, many currently available techniques used for this purpose, although sensitive and selective, require expensive equipment and advanced human skills and do not meet the demands of today’s clinic in terms of test time, simplicity and point-of-care applicability. In the course of ongoing pursuit of new analytical methodologies, electrochemical biosensing is steadily gaining ground as a strategy suitable to develop simple, low-cost methods, with the ability for multiplexed and multiomics determinations in a short time and requiring a small amount of sample. This review article puts forward electrochemical biosensing methods reported in the last five years for the determination of cytokines, summarizes recent developments and trends through a comprehensive discussion of selected strategies, and highlights the challenges to solve in this field. Considering the key role demonstrated in the last years by different materials (with nano or micrometric size and with or without magnetic properties), in the design of analytical performance-enhanced electrochemical biosensing strategies, special attention is paid to the methods exploiting these approaches.

Section: Bioelectroanalytical Methods For the Determination Of Infmentioning

confidence: 99%

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

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“…Sensitivity is an important parameter in each application that requires measuring small variations of the analytes of interest. Specific functionalization techniques are usually employed to enhance sensitivity and selectivity [ 7 ]. Selectivity is achieved by means of different materials able to bind analytes and/or catalyze the reactions.…”

Section: Introductionmentioning

confidence: 99%

3D Electrochemical Sensor and Microstructuration Using Aerosol Jet Printing

Fapanni

Sardini

Serpelloni

et al. 2021

Electrochemical sensors are attracting great interest for their different applications. To improve their performances, basic research focuses on two main issues: improve their metrological characteristics (e.g., repeatability, reusability and sensitivity) and investigate innovative fabrication processes. In this work, we demonstrate an innovative microstructuration technique aimed at increasing electrochemical sensor sensitivity to improve electrode active area by an innovative fabrication technique. The process is empowered by aerosol jet printing (AJP), an additive-manufacturing and non-contact printing technique that allows depositing functional inks in precise patterns such as parallel lines and grids. The 3D printed microstructures increased the active surface area by up to 130% without changing the substrate occupancy. Further, electrochemical detection of ferro/ferri-cyanide was used to evaluate the sensitivity of the electrodes. This evaluation points out a sensitivity increase of 2.3-fold on average between bare and fully microstructured devices. The increase of surface area and sensitivity are well linearly correlated as expected, verifying the fitness of our production process. The proposed microstructuration is a viable solution for many applications that requires high sensitivity, and the proposed technique, since it does not require masks or complex procedures, turns out to be flexible and applicable to infinite construction geometries.

“…Significant progress has been made towards establishing electrochemical biosensors in health-monitoring systems, yet only a few biosensors other than glucose monitors have found significant commercial application [ 1 ]. Most analytes outside of glucose and alike metabolites, such as proteins and hormones, maintain much lower biological concentrations than is detectable by electrochemical biosensors in their current state [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

A Dual Approach of an Oil–Membrane Composite and Boron-Doped Diamond Electrode to Mitigate Biofluid Interferences

DeBrosse

Yuan

Brothers

et al. 2021

Electrochemical biosensors promise a simple method to measure analytes for both point-of-care diagnostics and continuous, wearable biomarker monitors. In a liquid environment, detecting the analyte of interest must compete with other solutes that impact the background current, such as redox-active molecules, conductivity changes in the biofluid, water electrolysis, and electrode fouling. Multiple methods exist to overcome a few of these challenges, but not a comprehensive solution. Presented here is a combined boron-doped diamond electrode and oil–membrane protection approach that broadly mitigates the impact of biofluid interferents without a biorecognition element. The oil–membrane blocks the majority of interferents in biofluids that are hydrophilic while permitting passage of important hydrophobic analytes such as hormones and drugs. The boron-doped diamond then suppresses water electrolysis current and maintains peak electrochemical performance due to the foulant-mitigation benefits of the oil–membrane protection. Results show up to a 365-fold reduction in detection limits using the boron-doped diamond electrode material alone compared with traditional gold in the buffer. Combining the boron-doped diamond material with the oil–membrane protection scheme maintained these detection limits while exposed to human serum for 18 h.