Beyond the Extracellular Vesicles: Technical Hurdles, Achieved Goals and Current Challenges When Working on Adipose Cells

Gómez-Serrano, María; Ponath, Viviane; Preußer, Christian; Strandmann, Elke Pogge von

doi:10.3390/ijms22073362

Cited by 8 publications

(6 citation statements)

References 133 publications

(237 reference statements)

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“…Each method has advantages and limitations. Thus, selecting the proper technique may not be an easy task [288]. In what follows, we will discuss the most important aspects of each method.…”

Section: Methods For Exosome Isolationmentioning

confidence: 99%

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Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

et al. 2021

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Exosomes are extracellular vesicles released by cells, both constitutively and after cell activation, and are present in different types of biological fluid. Exosomes are involved in the pathogenesis of diseases, such as cancer, neurodegenerative diseases, pregnancy disorders and cardiovascular diseases, and have emerged as potential non-invasive biomarkers for the detection, prognosis and therapeutics of a myriad of diseases. In this review, we describe recent advances related to the regulatory mechanisms of exosome biogenesis, release and molecular composition, as well as their role in health and disease, and their potential use as disease biomarkers and therapeutic targets. In addition, the advantages and disadvantages of their main isolation methods, characterization and cargo analysis, as well as the experimental methods used for exosome-mediated drug delivery, are discussed. Finally, we present potential perspectives for the use of exosomes in future clinical practice.

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“…Each method has advantages and limitations. Thus, selecting the proper technique may not be an easy task [288]. In what follows, we will discuss the most important aspects of each method.…”

Section: Methods For Exosome Isolationmentioning

confidence: 99%

“…Ultracentrifugation is considered the gold standard for exosome isolation, and is based on the separation of the different components of the sample (cell media or bodily fluid) according to their density [288]. This is a two-step method.…”

Section: Ultracentrifugationmentioning

confidence: 99%

Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

et al. 2021

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“…Standardized methodological approaches to isolate and characterize EVs from adipose cells as well as from any other sources are still lacking [39]. Taking this limit into account, a variety of nucleic acids and proteins have been identified in ASC-derived EVs as important mediators of the paracrine function of ASCs [40].…”

Section: Characterization Of Evs Obtained From Ascsmentioning

confidence: 99%

Adipose Stromal/Stem Cell-Derived Extracellular Vesicles: Potential Next-Generation Anti-Obesity Agents

Zuccarini

Giuliani

Liberto

et al. 2022

IJMS

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Over the last decade, several compounds have been identified for the treatment of obesity. However, due to the complexity of the disease, many pharmacological interventions have raised concerns about their efficacy and safety. Therefore, it is important to discover new factors involved in the induction/progression of obesity. Adipose stromal/stem cells (ASCs), which are mostly isolated from subcutaneous adipose tissue, are the primary cells contributing to the expansion of fat mass. Like other cells, ASCs release nanoparticles known as extracellular vesicles (EVs), which are being actively studied for their potential applications in a variety of diseases. Here, we focused on the importance of the con-tribution of ASC-derived EVs in the regulation of metabolic processes. In addition, we outlined the advantages/disadvantages of the use of EVs as potential next-generation anti-obesity agents.

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“…It is important to highlight that future studies should analyze both the genetic content and bioactive proteins, to more accurately understand their role in the regulation of metabolism [ 25 ]. Other authors also highlight the need to standardize procedures for the study of ADEVs to avoid misinterpretations [ 107 ].…”

Section: Pi3k/akt/mtor Signaling Cascadementioning

confidence: 99%

Extracellular Vesicles from Adipose Tissue Could Promote Metabolic Adaptation through PI3K/Akt/mTOR

Delgadillo-Velázquez

Mendivil-Alvarado

Coronado-Alvarado

et al. 2022

Cells

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Extracellular vesicles (EVs) are nanoparticles secreted by cells under physiological and pathological conditions, such as metabolic diseases. In this context, EVs are considered potential key mediators in the physiopathology of obesity. It has been reported that EVs derived from adipose tissue (ADEVs) contribute to the development of a local inflammatory response that leads to adipose tissue dysfunction. In addition, it has been proposed that EVs are associated with the onset and progression of several obesity-related metabolic diseases such as insulin resistance. In particular, characterizing the molecular fingerprint of obesity-related ADEVs can provide a bigger picture that better reflects metabolic adaptation though PI3K/Akt/mTOR. Hence, in this review we describe the possible crosstalk communication of ADEVs with metabolically active organs and the intracellular response in the insulin signaling pathway.

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Beyond the Extracellular Vesicles: Technical Hurdles, Achieved Goals and Current Challenges When Working on Adipose Cells

Cited by 8 publications

References 133 publications

Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

Exosomes: Potential Disease Biomarkers and New Therapeutic Targets

Adipose Stromal/Stem Cell-Derived Extracellular Vesicles: Potential Next-Generation Anti-Obesity Agents

Extracellular Vesicles from Adipose Tissue Could Promote Metabolic Adaptation through PI3K/Akt/mTOR

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