Beyond the IFN-γ horizon: biomarkers for immunodiagnosis of infection with<i>Mycobacterium tuberculosis</i>

Chegou, Novel N.; Heyckendorf, Jan; Walzl, Gerhard; Lange, Christoph; Rühwald, Morten

doi:10.1183/09031936.00151413

Cited by 149 publications

(138 citation statements)

References 169 publications

(217 reference statements)

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“…One such readout is the cytokine IP-10, produced at much higher levels than interferon-gamma. Use of such a readout could both increase test sensitivity, particularly in immunocompromised groups, as well as reduce time to detection, bringing the test closer to true point of care testing (35).…”

Section: New Ltbi Diagnosticsmentioning

confidence: 99%

Latent tuberculosis infection: An overview

Kiazyk¹,

Ball²

2017

Canada Communicable Disease Report

167

109

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Latent tuberculosis infection (LTBI) is defined as a state of persistent immune response to stimulation by Mycobacterium tuberculosis antigens without evidence of clinically manifested active tuberculosis (TB) disease. Individuals with LTBI represent a reservoir for active TB cases. The detection and management of LTBI is now a key component of the World Health Organization's End TB Strategy and the Government of Canada's federal framework for action on TB prevention and control. This is because people with LTBI can progress to active TB or undergo reactivation, a risk that is greatly increased in those with immunocompromising conditions. This overview provides a summary of LTBI and reactivation risk, as well as the recent advances in the diagnosis and treatment of LTBI.

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Section: New Ltbi Diagnosticsmentioning

confidence: 99%

Latent tuberculosis infection: An overview

Kiazyk¹,

Ball²

2017

Canada Communicable Disease Report

167

109

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“…These studies have stimulated further interest in exploring cytokine profiling for other clinical applications such as improved identification of patients at risk for developing tuberculosis or for monitoring treatment success (30). Most studies have used flow-cytometric analysis of stimulation-induced cytokines after intracellular accumulation (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Superior Sensitivity of Ex Vivo IFN-γ Release Assays as Compared to Skin Testing in Immunocompromised Patients

Scholman

Straub

Sotgiu

et al. 2015

American Journal of Transplantation

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“…A significant difference in genotype frequencies was obtained when comparing the AA genotype between CTRL and ATB groups; AA homozygous indigenous individuals showed a relative risk of 0.98. The IFN-γ cytokine is the archetypical readout for cell-mediated immune response (CMI) assays 16 , and has been recognized as the defining cytokine of Th1 cells. IFN-γ-mediated immune activation appears to play an important role in immunity to intracellular pathogens, both in mice and humans [17][18][19] .…”

Section: Discussionmentioning

confidence: 99%

Concordance between IFNγ gene +874 A/T polymorphism and interferon-γ expression in a TB-endemic indigenous setting

Araujo

Palacios

Biomon

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Interferon-γ (IFN-γ) plays a crucial role in resistance to mycobacterial diseases; accordingly, variants of the gene encoding this cytokine may be associated with elevated risk of contracting pulmonary tuberculosis (TB). Methods: Blood samples were collected from 135 Warao indigenous individuals with newly diagnosed sputum culture-positive TB. Of these, 24 were diagnosed with active tuberculosis (ATB). The study comprised 111 participants, who were grouped as follows: 1) 14 tuberculin skin test (TST)-positive Warao indigenous individuals and 4 that were QuantiFERON-TBGold In-Tube (QFT-IT) test-positive, collectively comprising the latent TB infection group (LTBI), n = 18), and 2) healthy controls who were QFT-ITand TST-negative, comprising the control group (CTRL, n = 93). Detection of the IFN γ gene (IFNG) +874A/T polymorphism was performed via PCR and quantification of IFNG expression via qPCR. Results: Relative to indigenous and white Americans, ATB and CTRL groups had a higher frequency of the IFNG SNP (+874A): 23 (95.8%) and 108 (97.3%), respectively. Indigenous Warao individuals homozygous for the IFNG (+874) A allele exhibited 3.59-fold increased risk of developing TB (95% confidence interval, 2.60-4.96, p =0.0001). A decreased frequency of the AT genotype was observed in individuals with pulmonary TB (4.16%) and controls (0.90%). The frequency of the TT genotype was decreased among controls (1.80%); none of the patients with TB were found to have this genotype. The differences in IFNG expression between the groups, under unstimulated and stimulated conditions, were not statistically significant. Conclusions: Preliminary results demonstrate concordance between IFNG +874 A/A genotype and low expression of IFNG.

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Beyond the IFN-γ horizon: biomarkers for immunodiagnosis of infection withMycobacterium tuberculosis

Cited by 149 publications

References 169 publications

Latent tuberculosis infection: An overview

Latent tuberculosis infection: An overview

Superior Sensitivity of Ex Vivo IFN-γ Release Assays as Compared to Skin Testing in Immunocompromised Patients

Concordance between IFNγ gene +874 A/T polymorphism and interferon-γ expression in a TB-endemic indigenous setting

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