Bhallatakadi Ghrita: Development and evaluation with reference to Murcchana and Shata-Dhauta process

Abstract

Background Ayurveda is primarily based upon use of herbs either singly or in combination (polyherbal). The cow ghee (clarified butterfat) is considered as a precious base for preparing medicines in Ayurveda. Processing of ghee with plant ingredients is renowned for enhancing their therapeutic efficacy. Objective In present research work, the attempt was made to develop cow ghee based Polyherbal Bhallatakadi Ghrita formulations and evaluate them with reference to ‘ Murcchana ’ and ‘ Shata-Dhauta ’ process. Materials and methods The research plants were identified, procured, authenticated and processed. The extracts of plant materials were prepared and used for development of Polyherbal Bhallatakadi Ghrita (PHBG), Polyherbal Bhallatakadi Murcchita Ghrita and Polyherbal Bhallatakadi Shata-Dhauta Ghrita formulations as per Ayurvedic procedures. The prepared ghrita formulations were subjected to organoleptic (colour, odour, taste, appearance and touch), physicochemical (pH, viscosity, moisture content, specific gravity, refractive index, acid value, saponification value, iodine value, peroxide value, Rechert Meissl value and Polenske value) evaluation, in-vitro antioxidant and GC-MS analysis. The accelerated and real time stability studies were carried out to determine shelf life of ghrita formulations. Results The results of evaluations indicate that, developed PHBG formulations retained the organoleptic and physicochemical characteristics of ghee. The shelf life of formulations was found to be in the range of 1.6 to 3.3 years at accelerated and 2.2 to 3.8 years at real time stability conditions. All ghrita formulations exhibited antioxidant activity in dose dependent manner. Conclusion The standardization or evaluation of Polyherbal Bhallatakadi Ghrita formulations was found to be crucial for the establishment of a steady biological, chemical or simply a quality assurance profile of the drugs.

Development and evaluation of aphrodisiac potential of a classical ayurvedic formulation, ‘Kaamdev ghrita’ in rat model

“…The plain ghee and KG samples were analyzed for various organoleptic (color, odor, taste, texture, touch) and physicochemical parameters (acid value, peroxide value, iodine value, saponification value, unsaponifiable matter, extractive values, refractive index, and specific gravity) as per standard procedures described in reference books and published reports [ 2 , 3 , [14] , [15] , [16] ].…”

“…Antioxidant activity of freshly prepared test samples i.e. plain ghee and KG on the same day (by preparing the test solution in DMSO) was evaluated using various in-vitro methods [ 3 , [17] , [18] , [19] ]. The antioxidant potential was expressed as IC 50 i.e.…”

“…The hydrogen-donating ability of plain ghee and KG was measured using the established 1,1-diphenyl-2-picryl-hydrazyl (DPPH) method [ 3 , [17] , [18] , [19] ]. Briefly, 3.0 mL of 10–100 μg mL −1 concentration of test solutions and 1.0 mL of 0.1 mM solution of DPPH in ethanol were mixed, the reaction mixture was kept in the dark for 30 min and then absorbance was measured at 517 nm using UV spectrophotometer (UV-1700, Shimadzu Corporation, Japan).…”

“…Processing of ‘ ghee’ with plant material/materials i.e. Ghrita is well acclaimed for enhancing the therapeutic efficacy of plant ingredients [ 2 , 3 ]. Ghrita is one of the popular Ayurvedic formulations prepared by boiling cow ghee ( Sneha dravya ), a paste of crude plant powder in water ( Kalka ), fresh juice of plant ( Svarasa ) and water ( Drava dravya ) in specific proportions as per the formula and procedures are given in Ayurvedic texts [ 1 , 4 ].…”

Development and Validation of Chromatographic Method for the Standardization of Homeopathic Formulation of Syzygium Cumini

“…The total phenolic content was measured by the Folin-Ciocalteu Colorimetric method, 28 while the flavonoid content was determined by the aluminum nitrate method. 29,30 Gallic acid (10-70 mg/mL) and quercetin (10-70 mg/mL) were used as standards, and results are expressed as milligrams of gallic acid equivalents (GAE mg/mL of tincture) and as milligrams of quercetin equivalents (Q mg/mL of tincture), respectively.…”