Bi-enzyme sensor based on thick-film carbon electrode modified with electropolymerized tyramine

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

“…This behaviour owes to the distortion of the enzyme structure with consequent K Mapparent higher than K M obtained for ChE in solution [50,55,110]. An example of chemical immobilisation is based on a non conducting polymer electrosynthetised onto the electrode and after the enzyme was immobilised by crosslinking with glutaraldehyde [111,112]. Several immobilisations were carried out successfully using ChE immobilised by cross-linking method with glutaraldehyde vapour [113][114][115][116][117] or making an enzymatic membrane onto the electrode with ChE, Nafion® and glutaraldehyde [118,119].…”

Progress in Preparation and Application of Nanostructured Neurotransmitters Microsensors/Microelectrodes

“…The sensors responded to Ach E with a linear range from 1.0 × 10 −11 to 1.0 × 10 −6 mol/L, with a detection limit of 1.0 × 10 −12 mol/L. Suprun et al 47 fabricated a bi-enzyme biosensor successfully with butyrycholinesterase and horseradish catalase. The enzymes were immobilized in the polytyramine matrix by cross-linking with glutaraldehyde.…”

Carbon Nanotube-Based Cholinesterase Biosensors for the Detection of Pesticides

“…This method confers to the biosensor high working stability even if there is usually a decrease of the enzymatic affinity towards its substrate due to the distortion of the enzyme structure (Arduini et al, 2006 andVakurov, 2004). An example of chemical immobilization is based on a non-conducting polymer electrosynthetized onto the electrode after the enzyme was immobilized by crosslinking with glutaraldehyde (Curulli et al, 2001;Suprun et al, 2004). ChE immobilization was able to carry out by cross-linking method with glutaraldehyde vapour (Arkhypova et al, 2003(Arkhypova et al, , 2004(Arkhypova et al, and 2008.…”