Bibliography of Confocal Microscopy

Webb, Robert H.

doi:10.1007/978-0-387-45524-2_52

Cited by 4 publications

(2 citation statements)

References 282 publications

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“…Studying the dynamics of embryonic development using classic fluorescence methods can be difficult for several reasons. First, exposure to high-intensity light [72] or even visible light emissions [73][74][75] can damage cells and affect adversely oocytes and embryos. The second reason is the need to monitor the object for a certain period, from several hours to several days.…”

Section: Application Of Ultrashort Laser Pulses For Nonlinear Microscopymentioning

confidence: 99%

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

Ilina

Ситников

2021

Diagnostics

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Although the use of lasers in medical diagnosis and therapies, as well as in fundamental biomedical research is now almost routine, advanced laser sources and new laser-based methods continue to emerge. Due to the unique ability of ultrashort laser pulses to deposit energy into a microscopic volume in the bulk of a transparent material without disrupting the surrounding tissues, the ultrashort laser-based microsurgery of cells and subcellular components within structurally complex and fragile specimens such as embryos is becoming an important tool in developmental biology and reproductive medicine. In this review, we discuss the mechanisms of ultrashort laser pulse interaction with the matter, advantages of their application for oocyte and preimplantation embryo microsurgery (e.g., for oocyte/blastomere enucleation and embryonic cell fusion), as well as for nonlinear optical microscopy for studying the dynamics of embryonic development and embryo quality assessment. Moreover, we focus on ultrashort laser-based approaches and techniques that are increasingly being applied in the fundamental research and have the potential for successful translation into the IVF (in vitro fertilization) clinics, such as laser-mediated individual embryo labelling and controlled laser-assisted hatching.

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Section: Application Of Ultrashort Laser Pulses For Nonlinear Microscopymentioning

confidence: 99%

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

Ilina

Ситников

2021

Diagnostics

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“…Fluorescence microscopy has long been a popular modality in biomedical applications because of its high sensitivity and low background . However, the chemical specificity comes from labeling at the cost of phototoxicity and perturbation of the intrinsic behaviors, which are a great concern for functional imaging.…”

Section: Introductionmentioning

confidence: 99%

Electronic Preresonance Stimulated Raman Scattering Spectromicroscopy Using Multiple-Plate Continuum

Huang

Lee

et al. 2023

J. Phys. Chem. B

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Stimulated Raman scattering (SRS) spectromicroscopy is a powerful technique that enables label-free detection of chemical bonds with high specificity. However, the low Raman cross section due to typical far-electronic resonance excitation seriously restricts the sensitivity and undermines its application to bio-imaging. To address this bottleneck, the electronic preresonance (EPR) SRS technique has been developed to enhance the Raman signals by shifting the excitation frequency toward the molecular absorption. A fundamental weakness of the previous demonstration is the lack of dual-wavelength tunability, making EPR-SRS only applicable to a limited number of species in the proof-of-concept experiment. Here, we demonstrate the EPR-SRS spectromicroscopy using a multiple-plate continuum (MPC) light source able to examine a single vibration mode with independently adjustable pump and Stokes wavelengths. In our experiments, the CC vibration mode of Alexa 635 is interrogated by continuously scanning the pump-to-absorption frequency detuning throughout the entire EPR region enabled by MPC. The results exhibit 150-fold SRS signal enhancement and good agreement with the Albrecht A-term preresonance model. Signal enhancement is also observed in EPR-SRS images of the whole Drosophila brain stained with Alexa 635. With the improved sensitivity and potential to implement hyperspectral measurement, we envision that MPC-EPR-SRS spectromicroscopy can bring the Raman techniques closer to a routine in bio-imaging.

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2012

Fundamentals of Light Microscopy and Electronic Imaging

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Bibliography of Confocal Microscopy

Cited by 4 publications

References 282 publications

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

Electronic Preresonance Stimulated Raman Scattering Spectromicroscopy Using Multiple-Plate Continuum

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