Bidirectional Regulation of Mouse Embryonic Stem Cell Proliferation by Nicotine Is Mediated Through Wnt Signaling Pathway

Qu, Qinglan; Zhang, Fengrong; Zhang, Xiang; Yin, Weihong

doi:10.1177/1559325817739760

Cited by 16 publications

(13 citation statements)

References 33 publications

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“…Nicotine reduced the proliferation of human Wharton’s jelly mesenchymal stem cells [ 69 ], rat microglia [ 70 ] and periodontal ligament-derived stem cells [ 71 ]; it caused autophagy in the Leydig cells [ 72 ] and pyroptosis of human aortic endothelial cells (HAECs) [ 73 ]. Nicotine affects embryonic mouse stem cells in two ways: at low concentrations (0.01 and 0.1 μM) it stimulates, while at high concentrations (1 and 10 μM) it suppresses proliferation without affecting viability [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

α-Conotoxins and α-Cobratoxin Promote, while Lipoxygenase and Cyclooxygenase Inhibitors Suppress the Proliferation of Glioma C6 Cells

et al. 2021

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Among the brain tumors, glioma is the most common. In general, different biochemical mechanisms, involving nicotinic acetylcholine receptors (nAChRs) and the arachidonic acid cascade are involved in oncogenesis. Although the engagement of the latter in survival and proliferation of rat C6 glioma has been shown, there are practically no data about the presence and the role of nAChRs in C6 cells. In this work we studied the effects of nAChR antagonists, marine snail α-conotoxins and snake α-cobratoxin, on the survival and proliferation of C6 glioma cells. The effects of the lipoxygenase and cyclooxygenase inhibitors either alone or together with α-conotoxins and α-cobratoxin were studied in parallel. It was found that α-conotoxins and α-cobratoxin promoted the proliferation of C6 glioma cells, while nicotine had practically no effect at concentrations below 1 µL/mL. Nordihydroguaiaretic acid, a nonspecific lipoxygenase inhibitor, and baicalein, a 12-lipoxygenase inhibitor, exerted antiproliferative and cytotoxic effects on C6 cells. nAChR inhibitors weaken this effect after 24 h cultivation but produced no effects at longer times. Quantitative real-time polymerase chain reaction showed that mRNA for α4, α7, β2 and β4 subunits of nAChR were expressed in C6 glioma cells. This is the first indication for involvement of nAChRs in mechanisms of glioma cell proliferation.

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Section: Discussionmentioning

confidence: 99%

α-Conotoxins and α-Cobratoxin Promote, while Lipoxygenase and Cyclooxygenase Inhibitors Suppress the Proliferation of Glioma C6 Cells

et al. 2021

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“…According to the results of current study, it seems that CSC exhibit hormetic dose responses on behaviors of 2‐C57 and 6‐C57 cells including proliferation, DT, Bax/Bcl2 ratio, Bax , Bcl2, and Ahr expression. Studies have shown that nicotine display biphasic dose response on mESC proliferation …”

Section: Discussionmentioning

confidence: 99%

Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells

Assadollahi

Mohammadi

Fathi

et al. 2018

J of Cellular Biochemistry

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Background: Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts. They can be used as valuable experimental models to test the effects of drugs, chemicals, and environmental contaminants such as cigarette smoke condensate (CSC) on preimplantation embryo development.The aim of this study was to evaluate the effect of CSC on ESCs derived from mice with different genetic backgrounds and maternal ages. Methods:The study groups consisted of mouse ESCs (mESCs) obtained from three sources: blastocysts developed from fertilized oocytes of two-month-old (2-C57) and six-month-old (6-C57) C57BL/6 inbred mice and those developed from fertilized oocytes of two-month-old (2-NMRI) NMRI outbred mice. The groups of mESCs were exposed to 0.04, 4, and 40 μg/mL CSC. After exposure, we measured cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and real-time polymerase chain reaction for changes in expressions of Oct4, Sox2, Nanog, Ahr, Bax, Bcl2, TFAM, and POLG. The cell doubling time (DT) of these populations was also determined.Results: We observed that CSC changed proliferation and DT in the 2-C57 and 6-C57 cells. There was no change in 2-NMRI cells. Exposure to CSC caused changes in the gene expressions and induced apoptosis in all three cell lines.Conclusion: Based on the results of the study, it can be concluded that CSC has an effect on the viability, DT and gene expression patterns in mouse ESCs and its effects vary based on the genetic background and maternal age of isolated mouse ESCs.

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“…More than 8 million people die from tobacco use each year [ 62 ]. Previous studies have indicated that nicotine exhibits dose-dependent bidirectional regulation of mouse [ 63 ] and rat [ 64 ] stem cell proliferation. Moreover, long-term and/or continuous nicotine administration has been reported to have a deleterious effect on ischemic brain injury [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Nicotine supplementation enhances simulated game performance of archery athletes

Hung

Chen

et al. 2021

Journal of the International Society of Sports Nutrition

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Background Nicotine is beneficial to mood, arousal and cognition in humans. Due to the importance of cognitive functioning for archery athletes, we investigated the effects of nicotine supplementation on the cognitive abilities, heart rate variability (HRV), and sport performance of professional archers. Methods Eleven college archers were recruited and given 2 mg of nicotine supplementation (NIC group) and placebo (PLA group) in a crossover design. Results The results showed that at 30 min after the intake of nicotine gum, the “correct rejection” time in the NIC group was significantly lower than that of the PLA group (7.29 ± 0.87 vs. 8.23 ± 0.98 msec, p < 0.05). In addition, the NIC group completed the grooved pegboard test in a shorter time than the PLA group (48.76 ± 3.18 vs. 53.41 ± 4.05 s, p < 0.05), whereas motor reaction times were not different between the two groups. Saliva α-amylase activity was significantly lower after nicotine supplementation (p < 0.01) but increased immediately after the archery test in the NIC group (p < 0.05). In addition, nicotine supplementation significantly decreased HRV and increased the archery score (290.58 ± 10.09 vs. 298.05 ± 8.56, p < 0.01). Conclusions Nicotine enhances the performance of archery athletes by increasing cognitive function and stimulating the sympathetic adrenergic system.

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Bidirectional Regulation of Mouse Embryonic Stem Cell Proliferation by Nicotine Is Mediated Through Wnt Signaling Pathway

Cited by 16 publications

References 33 publications

α-Conotoxins and α-Cobratoxin Promote, while Lipoxygenase and Cyclooxygenase Inhibitors Suppress the Proliferation of Glioma C6 Cells

α-Conotoxins and α-Cobratoxin Promote, while Lipoxygenase and Cyclooxygenase Inhibitors Suppress the Proliferation of Glioma C6 Cells

Effects of cigarette smoke condensate on proliferation and pluripotency gene expression in mouse embryonic stem cells

Nicotine supplementation enhances simulated game performance of archery athletes

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