Bienzymatic Spectrophotometric Method for Uric Acid Estimation in Human Serum and Urine

Roopa, R.A.; Mantelingu, Kempegowda; Guin, Mridula; Thimmaiah, Sridhar Bilgumba

doi:10.1134/s1061934822030091

Cited by 5 publications

(1 citation statement)

References 40 publications

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“…Uric acid is the end-product of purine metabolism in the body and exists in the form of highly soluble urates [ 2 ]. The average levels of uric acid in serum and urine of healthy individuals ranged from 240 to 520 μM and 1.44 to 4.43 mM, respectively [ 3 ]. An increase or decrease in uric acid levels can trigger or reveal a range of diseases.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity Detection of Uric Acid and Creatinine in Human Urine Based on Nanoporous Gold

et al. 2022

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Given the significance of uric acid and creatinine in clinical diagnostic, disease prevention and treatment, a multifunctional electrochemical sensor was proposed for sensitive detection of uric acid and creatinine. The sensitive detection of uric acid was realized based on the unique electrochemical oxidation of nanoporous gold (NPG) towards uric acid, showing good linearity from 10 μM to 750 μM with a satisfactory sensitivity of 222.91 μA mM−1 cm−2 and a limit of detection (LOD) of 0.06 μM. Based on the Jaffé reaction between creatinine and picric acid, the sensitive detection of creatinine was indirectly achieved in a range from 10 to 2000 μM by determining the consumption of picric acid in the Jaffé reaction with a detection sensitivity of 195.05 μA mM−1 cm−2 and a LOD of 10 μM. For human urine detection using the proposed electrochemical sensor, the uric acid detection results were comparable to that of high-performance liquid chromatography (HPLC), with a deviation rate of less than 10.28% and the recoveries of uric acid spiked in urine samples were 89~118%. Compared with HPLC results, the deviation rate of creatinine detection in urine samples was less than 4.17% and the recoveries of creatinine spiked in urine samples ranged from 92.50% to 117.40%. The multifunctional electrochemical sensor exhibited many advantages in practical applications, including short detection time, high stability, simple operation, strong anti-interference ability, cost-effectiveness, and easy fabrication, which provided a promising alternative for urine analysis in clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Sensitivity Detection of Uric Acid and Creatinine in Human Urine Based on Nanoporous Gold

et al. 2022

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Oxygen‐Doped Graphitic Carbon Nitride for Electrochemical Uric Acid Sensing: Method Without Metals

Thakur,

Bhardwajj,

Taunk

et al. 2024

ChemistrySelect

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The present study examined potential benefits of heteroatom doping to manipulate the electrical structure and to enhance the efficiency of the sensor, with an emphasis on developing inexpensive, metal‐free electrochemical sensors. Melamine and ammonium oxalate were used as precursors in a one‐step thermal polymerization technique yielding oxygen‐doped graphitic carbon nitride (O‐gCN). Following synthesis, the material's structural and morphological properties were thoroughly examined using a multitude of analytical techniques, including EIS. A notable decrease in impedance was observed, suggesting improved conductivity. Additionally, the doping approach exhibited a noticeable improvement in reduction current receptivity and surface area as compared to unmodified graphitic carbon nitride (g‐CN). Utilizing both differential pulse voltammetry (DPV) and the classical cyclic voltammetry (CV) methods, we investigated the electro oxidation of uric acid (UA) as well as concentration dependence and selectivity studies using oxygen‐doped graphitic carbon nitride modified glassy carbon electrode (O‐gCN@GCE). O‐gCN@GCE demonstrated remarkable performance, allowing the detection of UA concentrations as low as 7.784 µAµM−1cm−2. Its low detection limit of 0.57 µM and wider linear range (5–120 µM) further highlighted its potential for extremely sensitive assays and its remarkable capacity to operate over a broad UA concentration range.

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Cationic cellulose dispersed Ag NCs/C-CNF paper-based SERS substrate with high homogeneity for creatinine and uric acid detection

Peng,

Yan,

You

et al. 2024

International Journal of Biological Macromolecules

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Bienzymatic Spectrophotometric Method for Uric Acid Estimation in Human Serum and Urine

Cited by 5 publications

References 40 publications

Sensitivity Detection of Uric Acid and Creatinine in Human Urine Based on Nanoporous Gold

Sensitivity Detection of Uric Acid and Creatinine in Human Urine Based on Nanoporous Gold

Oxygen‐Doped Graphitic Carbon Nitride for Electrochemical Uric Acid Sensing: Method Without Metals

Cationic cellulose dispersed Ag NCs/C-CNF paper-based SERS substrate with high homogeneity for creatinine and uric acid detection

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