Bifidobacterium animalis subsp. lactis ATCC 27673 Is a Genomically Unique Strain within Its Conserved Subspecies

Loquasto, Joseph R.; Barrangou, Rodolphe; Dudley, Edward G.; Stahl, Buffy; Chen, Chun; Roberts, Robert

doi:10.1128/aem.01777-13

Cited by 31 publications

(19 citation statements)

References 59 publications

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“…Advances in sequencing technologies, assembly, and annotation have enabled the scientific community to determine the complete genomes of probiotic strains (Altermann et al, 2005; Stahl and Barrangou, 2013), allowing the development of genotyping methods (Barrangou et al, 2009; Barrangou and Horvath, 2012), and providing unequivocal insights into the proper taxonomy of broadly used commercial strains (Makarova et al, 2006; Briczinski et al, 2009; Loquasto et al, 2013; Milani et al, 2013; Holzapfel and Wood, 2014; Lugli et al, 2014). Trends regarding the formulation of increasingly efficacious and complex blends of multiple probiotics in food and dietary supplements demand the development of high-resolution, yet affordable methods that enable the determination of bacterial counts, and their classification for proper labeling.…”

Section: Introductionmentioning

confidence: 99%

Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases

et al. 2016

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Recent advances in microbiome research have brought renewed focus on beneficial bacteria, many of which are available in food and dietary supplements. Although probiotics have historically been defined as microorganisms that convey health benefits when ingested in sufficient viable amounts, this description now includes the stipulation “well defined strains,” encompassing definitive taxonomy for consumer consideration and regulatory oversight. Here, we evaluated 52 commercial dietary supplements covering a range of labeled species using plate counting and targeted genotyping. Strain identities were assessed using methods recently published by the United States Pharmacopeial Convention. We also determined the relative abundance of individual bacteria by high-throughput sequencing (HTS) of the 16S rRNA sequence using paired-end 2 × 250 bp Illumina MiSeq technology. Using these methods, we tested the hypothesis that products do contain the quantitative and qualitative list of labeled microbial species. We found that 17 samples (33%) were below label claim for CFU prior to their expiration dates. A multiplexed-PCR scheme showed that only 30/52 (58%) of the products contained a correctly labeled classification, with issues encompassing incorrect taxonomy, missing species, and un-labeled species. The HTS revealed that many blended products consisted predominantly of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis. These results highlight the need for reliable methods to determine the correct taxonomy and quantify the relative amounts of mixed microbial populations in commercial probiotic products.

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Section: Introductionmentioning

confidence: 99%

Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases

et al. 2016

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“…Because they are more resistant to harsh environmental conditions, strains of B. animalis subsp. lactis have been studied more than those of other members of the genus Bifidobacterium, and clinical studies demonstrated their healthpromoting attributes in some gastrointestinal disorders and allergic processes (4)(5)(6).…”

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A Single Mutation in the Gene Responsible for the Mucoid Phenotype of Bifidobacterium animalis subsp. lactis Confers Surface and Functional Characteristics

Hidalgo-Cantabrana

Sánchez

Álvarez‐Martín

et al. 2015

Appl Environ Microbiol

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Exopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many of their roles have not yet been elucidated. We have sequenced the genomes of two isogenic strains of Bifidobacterium animalis subsp. lactis that differ in their EPS-producing phenotype. The original strain displays a nonmucoid appearance, and the mutant derived thereof has acquired a mucoid phenotype. The sequence analysis of their genomes revealed a nonsynonymous mutation in the gene Balat_1410, putatively involved in the elongation of the EPS chain. By comparing a strain from which this gene had been deleted with strains containing the wild-type and mutated genes, we were able to show that each strain displays different cell surface characteristics. The mucoid EPS synthesized by the strain harboring the mutation in Balat_1410 provided higher resistance to gastrointestinal conditions and increased the capability for adhesion to human enterocytes. In addition, the cytokine profiles of human peripheral blood mononuclear cells and ex vivo colon tissues suggest that the mucoid strain could have higher anti-inflammatory activity. Our findings provide relevant data on the function of Balat_1410 and reveal that the mucoid phenotype is able to alter some of the most relevant functional properties of the cells.T he genus Bifidobacterium includes commensal microorganisms commonly found in the human gut. Some strains, belonging mainly to the species Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium bifidum, and Bifidobacterium animalis, have been used as probiotics, since there is scientific evidence that links the administration of these bacteria with specific health benefits (1, 2). In particular, B. animalis subsp. lactis has a robust phenotype that allows growth at high numbers in commercial applications under nonanaerobic conditions. Furthermore, strains of this subspecies survive the gastric passage and reach the intestinal tract in a metabolically active state, being also the most common representatives of bifidobacteria in functional food products (3). Because they are more resistant to harsh environmental conditions, strains of B. animalis subsp. lactis have been studied more than those of other members of the genus Bifidobacterium, and clinical studies demonstrated their healthpromoting attributes in some gastrointestinal disorders and allergic processes (4-6).Exopolysaccharides (EPS) are extracellular layers of carbohydrates typically found in microorganisms (7). EPS production has been involved in a variety of physiological processes that have implications for the producer microorganism and the host (8, 9). Specifically, in intestinal bacteria some of the most relevant roles attributed to EPS are the favoring of intestinal colonization and survival (10-12) and immunomodulation (11,13,14).A common set of functional modules, including the synthesis of activated sugar precursors, cytoplasmic assembly of the EPS unit, export...

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“…lactis ATCC 27673, which clustered apart with a 100% posterior probability. It has been reported that strain ATCC 27673 harbors unique genome traits into genomic islands, making this strain very different from the rest (Loquasto et al, 2013).…”

Section: Strain Bioprojectmentioning

confidence: 99%

A peptidome-based phylogeny pipeline reveals differential peptides at the strain level within Bifidobacterium animalis subsp. lactis

et al. 2016

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Bifidobacteria are gut commensal microorganisms belonging to the Actinobacteria group. Some specific strains of Bifidobacterium animalis subsp. lactis are used in functional foods as they are able to exert health-promoting effects in the human host. Due to the limited genetic variability within this subspecies, it is sometimes difficult for a manufacturer to properly track its strain once included in dairy products or functional foods. In this paper, we present a peptidome-based analysis in which the proteomes of a set of B. animalis subsp. lactis strains were digested in silico with human gut endopeptidases. The molecular masses were compared along all the strains to detect strain-specific peptides. These peptides may be interesting towards the development of methodologies for strain identification in the final product.

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Bifidobacterium animalis subsp. lactis ATCC 27673 Is a Genomically Unique Strain within Its Conserved Subspecies

Cited by 31 publications

References 59 publications

Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases

Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases

A Single Mutation in the Gene Responsible for the Mucoid Phenotype of Bifidobacterium animalis subsp. lactis Confers Surface and Functional Characteristics

A peptidome-based phylogeny pipeline reveals differential peptides at the strain level within Bifidobacterium animalis subsp. lactis

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