Bifunctional peptides that precisely biomineralize Au clusters and specifically stain cell nuclei

Wang, Yaling; Cui, Yanyan; Zhao, Yuliang; Liu, Ru; Sun, Zhipeng; Li, Wei; Gao, Xueyun

doi:10.1039/c1cc15926g

Cited by 138 publications

(130 citation statements)

References 30 publications

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“…Studies on peptides showed that the coexistence of cysteine and tyrosine in peptides can determine if a biomolecule can potentially mediate the formation of photoluminescent, red-emitting gold clusters. These observations provide experimental evidence for earlier assumptions [3,14]. Furthermore, our results showed that effectiveness of cysteine residues is not hindered by their disulfide-bonded state.…”

Section: Discussionsupporting

confidence: 76%

“…We observed the same effect when tyrosine was replaced by tryptophan. These results revealed that cysteine and tyrosine are exclusively responsible for cluster formation and cannot be substituted by other residues, which is an experimental evidence for earlier assumptions [3,14]. Cysteine might be essential in cluster formation due to the electron withdrawing effect of the thiol-gold bond, which plays a crucial role in cluster stabilization [44].…”

Section: Preparation Of Auqcs With Peptidessupporting

confidence: 57%

“…Subnanometer-sized gold particles show photoluminescence emission that can be tuned in a wide range of the spectrum, from ultraviolet through visible to the near infrared [2]. These clusters show good photostability compared to organic fluorophores [3]. Furthermore, unlike semiconductor quantum dots [4], gold clusters do not have the disadvantage of susceptibility to oxidation.…”

Section: Introductionmentioning

confidence: 99%

“…In the literature, AuQC synthesizing activity of peptides has not been widely investigated. To date, only a single artificial peptide (CCYRGRKKRRQRRR) has been designed especially for AuQC production [3].…”

Section: Introductionmentioning

confidence: 99%

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On the selection and design of proteins and peptide derivatives for the production of photoluminescent, red-emitting gold quantum clusters

et al. 2013

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Novel pathways of the synthesis of photoluminescent gold quantum clusters (AuQCs) using biomolecules as reactants provide biocompatible products for biological imaging techniques. In order to rationalize the rules for the preparation of red-emitting AuQCs in aqueous phase using proteins or peptides, the role of different organic structural units was investigated. Three systems were studied: proteins, peptides, and amino acid mixtures, respectively. We have found that cysteine and tyrosine are indispensable residues. The SH/S-S ratio in a single molecule is not a critical factor in the synthesis, but on the other hand, the stoichiometry of cysteine residues and the gold precursor is crucial. These observations indicate the importance of proper chemical behavior of all species in a wide size range extending from the atomic distances (in the Au I -S semi ring) to nanometer distances covering the larger sizes of proteins assuring the hierarchical structure of the whole self-assembled system.

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Section: Discussionsupporting

confidence: 76%

Section: Preparation Of Auqcs With Peptidessupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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On the selection and design of proteins and peptide derivatives for the production of photoluminescent, red-emitting gold quantum clusters

et al. 2013

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“…The quantum efficiency was determined to be ∼12.3% (Fig. S3), which was similar to those reported for peptide-templated AuNCs (Gu et al, 2014;Wang et al, 2012). The transmission electron microscope (TEM) was used to directly observe the morphology of the peptide-AuNCs.…”

Section: Characterization Of Peptide-auncsmentioning

confidence: 98%

Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe

Song

Wang

Liang

et al. 2015

Biosensors and Bioelectronics

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Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

Mok

Loh

et al. 2016

Adv Healthcare Materials

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thiolate-protected, [ 3 ] protein/peptide-templated, [ 4 ] and DNA-templated [ 5 ] ones. The last two types of metal NCs use biomolecules as templates to direct the synthesis (thus termed as biotemplated metal NCs), which afford more attractive features such as good aqueous solubility, superb biocompatibility, and versatile surface chemistries, [ 6 ] in addition to their ultrasmall size and superior photoluminescent optical properties. These desirable features render biotemplated metal NCs as promising candidates for various biomedical applications like imaging, [ 7 ] sensing, [ 7b,8 ] delivery [ 9 ] and therapeutics. [ 10 ] Theranostics is a revolutionary treatment strategy proposed by Funkhouser in 1998, which combines accurate diagnosis and targeted therapeutics. [ 11 ] From the list of individal biomedical applications mentioned earlier, a combination of sensing and imaging modalities would be fundamental for disease diagnosis. [ 12 ] In the same vein, image-guided targetedtherapy allows for real-time in vivo monitoring of the pharmacokinetics and pharmacodynamics of the therapeutic agent to enhance treatment effi ciency. [ 13 ] Through the ingenious combination of selective modalities, a highly potent and personalized theranostic agent could be developed. In particular, NC-based systems are poised to be the next versatile theranostic platform. This is because the intrinsic photoluminescence of the noble metal core can be harnessed for fl uorescence imaging, and tailor-made to exhibit unique optical properties for therapy, such as the generation of singlet oxygen [ 14 ] to substitute organicbased photosensitizers for possible photodynamic cancer treatments. In addition to the metal core, the biomolecular ligand shell surrounding the metal NC core can be easily functionalized with biorecognition moieties for sensing and targeted delivery. Due to the large surface to volume ratio of metal NCs, it can also serve as a reservoir sink to load multiple drugs/ genes and be modifi ed with sensing/imaging agents to imbue both therapeutic and diagnostic functions.The selective marriage of respective modalities to form theranostic biotemplated metal NCs will have a far-reaching impact on the future of medicine and healthcare. There are large amounts of work that use randomly picked natural biomolecules as templates to synthesize metal NCs for biomedical

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Bifunctional peptides that precisely biomineralize Au clusters and specifically stain cell nuclei

Abstract: A bifunctional peptide containing a domain that targets cell nuclei and a domain with the ability to biomineralize and capture Au clusters is presented. The peptide-Au clusters exhibit red emission (λ(em) = 677 nm) and specifically stain the nuclei of three cell lines.

Cited by 138 publications

References 30 publications

On the selection and design of proteins and peptide derivatives for the production of photoluminescent, red-emitting gold quantum clusters

On the selection and design of proteins and peptide derivatives for the production of photoluminescent, red-emitting gold quantum clusters

Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe

Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

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