Bifunctionalized dextrans for surface PEGylation via multivalent host–guest interactions

Antoniuk, Iurii; Wintgens, Véronique; Volet, Gisèle; Nielsen, Thorbjørn Terndrup

doi:10.1016/j.carbpol.2015.07.027

Cited by 4 publications

(4 citation statements)

References 41 publications

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“…Approximating dextran as a linear polymer composed of α‐1,6 linked glucose units, GPE is possibly reacting with one out of the three free hydroxyl functions (O2, O3, O4). The precise hydroxyl function involved in the new ether bond formation is the object of an open debate [20,22,23] . The less hindered position is O3, however the H1 chemical shift of the newly modified units likely corresponds to O2/O4 functionalized glucose.…”

Section: Resultsmentioning

confidence: 99%

“…The precise hydroxyl function involved in the new ether bond formation is the object of an open debate. [ 20 , 22 , 23 ] The less hindered position is O3, however the H1 chemical shift of the newly modified units likely corresponds to O2/O4 functionalized glucose. The hydroxyl at position 2 of the glucose unit is generally shown to be the most substituted in these conditions.…”

Section: Resultsmentioning

confidence: 99%

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Selective Capture of Anti‐N‐glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate

et al. 2021

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Tentacle‐like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity. We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non‐typeable Haemophilus influenzae (NTHi). We selected the C‐terminal HMW1ct(1347–1354) minimal epitope and prepared the diglucosylated analogue Ac‐KAN(Glc)VTLN(Glc)TTG‐K(N3)‐NH2 to graft a 40 kDa dextran scaffold modified with glycidyl‐propargyl moieties to perform a copper catalyzed alkyne‐azide coupling reaction (CuAAC). Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate. This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera. Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA. These results may offer a promising proof‐of‐concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Selective Capture of Anti‐N‐glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate

et al. 2021

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“…15 The fabrication of nanostructured materials with a high degree of order and a low incidence of defects by self-assembly is a promising technique, which has been widely used in the construction of nanostructured materials with different morphologies. [16][17][18] Nanostructured materials formed by selfassembly have unique physical and chemical properties, and they can be used as smart drug carrier materials. However, self-assembly is usually a spontaneous thermodynamic process, which is difficult for humans to interfere with and control.…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrin-based biological stimuli-responsive carriers for smart and precision medicine

Liao

Wang

et al. 2017

Biomater. Sci.

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Spurred on by recent progress in nanotechnology and precision medicine, smart drug carriers are entering an entirely new era. Smart drug carriers have been widely studied in recent years as a result of their ability to control drug release under different microenvironments (such as pH, redox, and enzyme) in vivo. Host-guest interactions based on cyclodextrins have proven to be an efficient tool for fabricating smart drug carriers. Because of the application of host-guest interactions, many kinds of biological molecules or supramolecular building blocks can combine into an organic whole at the molecular level. In this review, the features, mechanisms of action, and potent applications of biological stimuli-responsive drug carriers based on cyclodextrins are discussed. In addition, some personal perspectives on this field are presented.

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“…These PEG-adamantyl-grafted b-CDs can form an interesting new class of materials in biomedical applications where reversible PEGylation is desired. 99 Recently, Chen et al reported on the regulation of protein binding capability of surfaces via b-CD host-guest interactions by varying the localized and average ligand density. It is long known that the ligand presentation at interfaces has a significant influence on their interaction with biomolecules and subsequent biological responses.…”

Section: Host-guest Complexes Based On Cyclodextrinmentioning

confidence: 99%

Advances in the Development of Supramolecular Polymeric Biomaterials

Goor

Dankers²

2017

Comprehensive Supramolecular Chemistry II

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Bifunctionalized dextrans for surface PEGylation via multivalent host–guest interactions

Cited by 4 publications

References 41 publications

Selective Capture of Anti‐N‐glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate

Selective Capture of Anti‐N‐glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate

Cyclodextrin-based biological stimuli-responsive carriers for smart and precision medicine

Advances in the Development of Supramolecular Polymeric Biomaterials

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