Bilateral limbal stem cell deficiency with chromosomal translocation of 3p and 9p

Usui, Tomohiro; Nakagawa, Suguru; Yokoo, Seiichi; Mimura, Tatsuya; Yamagami, Satoru; Amano, Shiro

doi:10.1007/s10384-010-0814-0

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…We did not utilize amniotic membrane in our SOMET procedure. Amniotic membrane has previously been used in a wide variety of ocular surface reconstruction techniques, such as amniotic membrane transplantation 5 , limbal transplantation 10,11 , cultured corneal epithelial sheet transplantation 13 , cultured conjunctival epithelial cell sheet transplantation 14 , COMET 15–19 , and SLET 20 . However, we found that using fibrin glue with human amniotic membrane induced severe ocular surface inflammation after transplantation in rabbits (unpublished observation 2017, Inamochi A).…”

Section: Discussionmentioning

confidence: 99%

“…Stem cell-based reconstruction of the ocular surface for LSCD has progressed from application of allografts such as conjunctival epithelial grafts 8,9 and limbal transplantation 10,11 to use of autografts for avoidance of rejection, including corneal limbal transplantation 12 and cultured corneal epithelial sheet transplantation 13 in patients with unilateral LSCD. In addition, bilateral LSCD has been treated by transplantation of cultured conjunctival epithelial cell sheets 14 and cultured oral mucosal epithelial cell sheets 15–19 . Among these techniques, transplantation of cultured cell sheets reduces the amount of donor tissue required and allows early rehabilitation after surgery.…”

Section: Introductionmentioning

confidence: 99%

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Simple oral mucosal epithelial transplantation in a rabbit model

Inamochi

Tomioka

Kitamoto

et al. 2019

Sci Rep

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This study investigated a rabbit model of autologous simple oral mucosal epithelium transplantation (SOMET) for limbal stem cell deficiency (LSCD). LSCD was created in the SOMET group and the Control group. In the SOMET group, oral mucosa harvested from the buccal region was treated with dispase, cut into small pieces, and placed on the exposed corneal stroma without using graft sutures, amniotic membrane, and/or glue. A soft contact lens was positioned and tarsorrhaphy was performed in both groups. Postoperative corneal neovascularization and fluorescein staining scores were evaluated by slit lamp microscopy in both groups. At 2 weeks postoperatively, eyes were excised and subjected to immunohistochemical staining for CK3, CK13, CK15, and p63. In the SOMET group, transplantation of oral mucosa led to complete recovery of LSCD, as indicated by low neovascularization scores, low fluorescein staining scores, and detection of stratified K3/K13-positive cells on the stroma at 2 weeks after surgery. In contrast, corneal epithelial defects persisted in the Control group at 2 weeks. SOMET achieved re-epithelialization of the corneal surface in this rabbit LSCD model. It is a simple technique that does not require culture and could be a promising option for ocular surface reconstruction in bilateral LSCD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simple oral mucosal epithelial transplantation in a rabbit model

Inamochi

Tomioka

Kitamoto

et al. 2019

Sci Rep

Self Cite

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Limbal Epithelial Cell Therapy: Past, Present, and Future

Utheim

2013

Methods in Molecular Biology

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The cornea, the clear window at the front of the eye, transmits light to the retina to enable vision. The corneal surface is renewed by stem cells located at the peripheral limbal region. These cells can be destroyed by a number of factors, including chemical burns, infections, and autoimmune diseases, which result in limbal stem cell deficiency (LSCD), a condition that can lead to blindness. Established therapy for LSCD based on ex vivo expanded limbal epithelial cells is currently at a stage of refinement. Therapy for LSCD is also rapidly evolving to include alternative cell types and clinical approaches as treatment modalities. In the present perspectives chapter, strategies to treat LSCD are discussed and advances in this important field of regenerative medicine are highlighted.

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Autologous transplantation of conjunctiva by modifying simple limbal epithelial transplantation for limbal stem cell deficiency

Sakimoto

Yamagami

2019

Jpn J Ophthalmol

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Bilateral limbal stem cell deficiency with chromosomal translocation of 3p and 9p

Cited by 5 publications

References 4 publications

Simple oral mucosal epithelial transplantation in a rabbit model

Simple oral mucosal epithelial transplantation in a rabbit model

Limbal Epithelial Cell Therapy: Past, Present, and Future

Autologous transplantation of conjunctiva by modifying simple limbal epithelial transplantation for limbal stem cell deficiency

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