Bile acids LCA and CDCA inhibited porcine deltacoronavirus replication in vitro

Kong, Fanzhi; Niu, Xiaoyu; Liu, Mingde; Wang, Qiuhong

doi:10.1016/j.vetmic.2021.109097

Cited by 24 publications

(14 citation statements)

References 82 publications

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“…Bile acids chenodeoxycholic acid and lithocholic acid suppress PDCoV replication at post-entry stages by inducing the production of IFN-λ3 and IFN-stimulated gene 15 (ISG15). Lithocholic acid functions through a G protein-coupled receptor-IFN-λ3-ISG15 signaling axis in IPEC-J2 cells ( 89 ). Melatonin and structural analogs, including indole, tryptamine and L-tryptophan possess antiviral activities against PDCoV.…”

Section: Current Anti-pdcov Strategiesmentioning

confidence: 99%

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Duan

2022

Front. Vet. Sci.

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The recent experience with SARS-COV-2 has raised our alarm about the cross-species transmissibility of coronaviruses and the emergence of new coronaviruses. Knowledge of this family of viruses needs to be constantly updated. Porcine deltacoronavirus (PDCoV), a newly emerging member of the genus Deltacoronavirus in the family Coronaviridae, is a swine enteropathogen that causes diarrhea in pigs and may lead to death in severe cases. Since PDCoV diarrhea first broke out in the United States in early 2014, PDCoV has been detected in many countries, such as South Korea, Japan and China. More importantly, PDCoV can also infect species other than pigs, and infections have even been reported in children, highlighting its potential for cross-species transmission. A thorough and systematic knowledge of the epidemiology and pathogenesis of PDCoV will not only help us control PDCoV infection, but also enable us to discover the common cellular pathways and key factors of coronaviruses. In this review, we summarize the current knowledge on the prevalence, pathogenicity and infection dynamics, pathogenesis and immune evasion strategies of PDCoV. The existing anti-PDCoV strategies and corresponding mechanisms of PDCoV infection are also introduced, aiming to provide suggestions for the prevention and treatment of PDCoV and zoonotic diseases.

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Section: Current Anti-pdcov Strategiesmentioning

confidence: 99%

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Duan

2022

Front. Vet. Sci.

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“…Very few studies have focused on how BAs influence replication of swine enteric coronaviruses (SECoVs), though it was recently demonstrated that BAs increased infectivity of porcine epidemic diarrhea coronavirus (PEDV) strain icPC22A in Vero cells and the porcine small intestinal epithelial cell line IPEC-DQ [14]. However, a later study reported that BAs had antiviral activity against another SECoV, porcine deltacoronavirus (PDCoV), reducing its replication in LLC-PK1 and IPEC-J2 cells [15]. These seemingly contradictory outcomes suggest that BAs may modulate replication of distinct SECoVs very differently.…”

Section: Introductionmentioning

confidence: 99%

Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids

Yang

Tang

et al. 2022

PLoS Pathog

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Intestinal microbial metabolites have been increasingly recognized as important regulators of enteric viral infection. However, very little information is available about which specific microbiota-derived metabolites are crucial for swine enteric coronavirus (SECoV) infection in vivo. Using swine acute diarrhea syndrome (SADS)-CoV as a model, we were able to identify a greatly altered bile acid (BA) profile in the small intestine of infected piglets by untargeted metabolomic analysis. Using a newly established ex vivo model–the stem cell-derived porcine intestinal enteroid (PIE) culture–we demonstrated that certain BAs, cholic acid (CA) in particular, enhance SADS-CoV replication by acting on PIEs at the early phase of infection. We ruled out the possibility that CA exerts an augmenting effect on viral replication through classic farnesoid X receptor or Takeda G protein-coupled receptor 5 signaling, innate immune suppression or viral attachment. BA induced multiple cellular responses including rapid changes in caveolae-mediated endocytosis, endosomal acidification and dynamics of the endosomal/lysosomal system that are critical for SADS-CoV replication. Thus, our findings shed light on how SECoVs exploit microbiome-derived metabolite BAs to swiftly establish viral infection and accelerate replication within the intestinal microenvironment.

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“…Consistent with our findings, Liang et al [34] recently observed that PDCoV inoculated 4-day-old SPF chickens exhibited no clinical signs, and their body temperatures were the same as control group. More recently an in vitro study found that PDCoV replication was inhibited by bile acids chenodeoxycholic acid (CDCA) and lithocholic acid (LCA) due to their antiviral activity by stimulating interferons, λ3 and ISG15 [54]. Furthermore, none of the cloacal and tracheal swabs of inoculated birds and sentinel birds were clearly positive using RT-qPCR.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Cross-Species Transmission Potential for Porcine Deltacoronaviruses Expressing Sparrow Coronavirus Spike Protein in Commercial Poultry

Alhamo

Boley

Liu

et al. 2022

Viruses

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Avian species often serve as transmission vectors and sources of recombination for viral infections due to their ability to travel vast distances and their gregarious behaviors. Recently a novel deltacoronavirus (DCoV) was identified in sparrows. Sparrow deltacoronavirus (SpDCoV), coupled with close contact between sparrows and swine carrying porcine deltacoronavirus (PDCoV) may facilitate recombination of DCoVs resulting in novel CoV variants. We hypothesized that the spike (S) protein or receptor-binding domain (RBD) from sparrow coronaviruses (SpCoVs) may enhance infection in poultry. We used recombinant chimeric viruses, which express S protein or the RBD of SpCoV (icPDCoV-SHKU17, and icPDCoV-RBDISU) on the genomic backbone of an infectious clone of PDCoV (icPDCoV). Chimeric viruses were utilized to infect chicken derived DF-1 cells, turkey poults, and embryonated chicken eggs (ECEs) to examine permissiveness, viral replication kinetics, pathogenesis and pathology. We demonstrated that DF-1 cells in addition to the positive control LLC-PK1 cells are susceptible to SpCoV spike- and RBD- recombinant chimeric virus infections. However, the replication of chimeric viruses in DF-1 cells, but not LLC-PK1 cells, was inefficient. Inoculated 8-day-old turkey poults appeared resistant to icPDCoV-, icPDCoV-SHKU17- and icPDCoV-RBDISU virus infections. In 5-day-old ECEs, significant mortality was observed in PDCoV inoculated eggs with less in the spike chimeras, while in 11-day-old ECEs there was no evidence of viral replication, suggesting that PDCoV is better adapted to cross species infection and differentiated ECE cells are not susceptible to PDCoV infection. Collectively, we demonstrate that the SpCoV chimeric viruses are not more infectious in turkeys, nor ECEs than wild type PDCoV. Therefore, understanding the cell and host factors that contribute to resistance to PDCoV and avian-swine chimeric virus infections may aid in the design of novel antiviral therapies against DCoVs.

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Bile acids LCA and CDCA inhibited porcine deltacoronavirus replication in vitro

Cited by 24 publications

References 82 publications

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids

Characterization of the Cross-Species Transmission Potential for Porcine Deltacoronaviruses Expressing Sparrow Coronavirus Spike Protein in Commercial Poultry

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