Bile Duct Exclusion From Selective Vascular Inflow Occlusion in Rat Liver: Role of Ischemic Preconditioning and N-Acetylcysteine on Hepatic Reperfusion Injury

Montero, Efs; Junior, Claudemiro Quireze; dʼOliveira, D R

doi:10.1097/00007890-200407271-00582

Cited by 13 publications

(23 citation statements)

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“…Many experimental studies in rats have shown that IPC reduces the intensity of the ischemic lesions 8,13 . The analyze of mucosal injury is considered a gold standard method when it wants to examine the effectiveness of preservation methods and is the most commonly used parameter to assess the quality of the intestinal graft under cold storage.…”

Section: Effects Of Ischemic Preconditioning Associated To Different mentioning

confidence: 99%

Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft

Neves¹,

Abrahão

Netto

et al. 2011

Acta Cir. Bras.

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PuRPOsE: To evaluate the effects of ischemic preconditioning (IPC) associate with different preservation solutions, in the protecting of gut. METHOds: Four groups of 14 rats underwent laparotomy and collecting 20 cm of ileum, for preservation, at 4 0 C, in Belzer (Belz), Ringer (RL), Celsior (Cs) and Custodiol (Cust) solutions, for 24 hours. Prior to collection, half of the animals in each group were subjected to IPC. During preservation, in the periods of zero, 12, 18 and 24 hours, were conducted evaluating the degree of mucosal injury and dosage of malondialdehyde acid (MDA). REsuLTs: In all periods the RL group, with and without IPC, presented MDA values higher than the Belz and Cs. The degree of mucosal injury in the non-ipc RLgroup with 12h preservation was higher than the others; with 18 and 24h, the RL and Cust had higher degrees of damage than Cs and Belz. With IPC, in all periods, the group Cs and Belz had lower degrees of injury. CONCLusION: The Celsior and Belzer solutions had better protective effects on the gut and these effects were enhanced by IPC. Keywords: Organ Preservation Solutions. Ischemic Preconditioning. Intestine, Small. Rats. REsuMO OBJETIVO: Avaliar os efeitos do precondicionamento isquêmico (PCI) associado a diferentes soluções de preservação, na proteção do intestino delgado. MéTOdOs: Quatro grupos de 14 ratos Wistar, foram submetidos à laparotomia e coleta de 20 cm de íleo, para preservação, a 4 0 C, nas soluções de Belzer (Belz), Ringer (RL), Celsior (Cs) e Custodiol (Cust) por 24 horas. Previamente à coleta, em metade dos animais de cada grupo, o intestino foi submetido ao PCI. Durante a preservação, nos períodos de Zero, 12, 18 e 24 horas, foram realizados avaliação do grau de lesão da mucosa e dosagem do ácido malondialdeído (MDA). REsuLTAdOs: Em todos os períodos o grupo RL, sem e com pci, apresentou valores maiores de MDA do que o Belz e Cs. O grau de lesão da mucosa nos grupos sem pci com preservação de 12h, no grupo RL, foi maior que nos demais; com 18h e 24h o grupo RL e Cust apresentaram maiores graus de lesão do que Cs e Belz. Com o pci, em todos os períodos, os grupos Belz e Cs apresentaram menores graus de lesão CONCLusãO: As Soluções Celsior e Belzer tiveram melhores efeitos na proteção do intestino e estes efeitos foram incrementados pelo precondicionamento isquêmico. descritores: Soluções para Preservação de Órgãos. Precondicionamento Isquêmico. Intestino Delgado. Ratos.

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Section: Effects Of Ischemic Preconditioning Associated To Different mentioning

confidence: 99%

Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft

Neves¹,

Abrahão

Netto

et al. 2011

Acta Cir. Bras.

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“…NAC is a low molecular weight thiol, it is derived from cysteine and acts removing reacting species of oxygen, besides improving the action of nitric oxide, acting on the regulation of microcirculation [11][12][13] . Usta et al 14 showed NAC improved tissue recovering in ovaries, which had been submitted to torsion and distortion.…”

Section: Introductionmentioning

confidence: 99%

N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

et al. 2014

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PURPOSE:To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC. METHODS:Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) -treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4 th or 15 th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test. RESULTS:At 4 th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15 th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation. CONCLUSION:In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle.

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“…Donor organ preconditioning (DOP) is an additive approach for donor liver protection and is thought to attenuate I/R injury after transplantation [7][8][9]. Basically, the achievement of DOP is attributed to the fact that drugs are applied under physiological conditions which guarantee adequate amounts of energy-rich phosphates necessary for the intracellular drug uptake [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…N-Acetylcysteine (NAC) is a thiol-containing compound that serves as a glutathione precursor [9]. It also can serve directly as a scavenger of oxygen-derived free radicals [18].…”

Section: Introductionmentioning

confidence: 99%

Multidrug donor preconditioning prevents cold liver preservation and reperfusion injury

Moussavian

Scheuer

Schmidt

et al. 2010

Langenbecks Arch Surg

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Bile Duct Exclusion From Selective Vascular Inflow Occlusion in Rat Liver: Role of Ischemic Preconditioning and N-Acetylcysteine on Hepatic Reperfusion Injury

Cited by 13 publications

References 0 publications

Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft

Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft

N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats

Multidrug donor preconditioning prevents cold liver preservation and reperfusion injury

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